LOCUS       40924_21583        9574 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGFP5'cog+, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9574)
  AUTHORS   Bowring FJ, Yeadon PJ, Catcheside DE.
  TITLE     Use of fluorescent protein to analyse recombination at three loci in
            Neurospora crassa
  JOURNAL   Fungal Genet. Biol. 49 (8), 619-625 (2012)
  PUBMED    22691725
REFERENCE   2  (bases 1 to 9574)
  AUTHORS   Bowring FJ, Yeadon J, Catcheside DEA.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUN-2012) School of Biological Sciences, Flinders 
            University, Sturt Road, Bedford Park, SA 5162, Australia
REFERENCE   3  (bases 1 to 9574)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9574)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet. Biol."; date: "2012"; volume: "49"; issue: "8"; pages: 
            "619-625"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-JUN-2012) School of Biological Sciences, Flinders University, 
            Sturt Road, Bedford Park, SA 5162, Australia"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9574
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        363..467
                     /label=AmpR promoter
     CDS             468..1325
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1499..2087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2345..2363
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     4904..4920
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             6126..6824
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="ELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTT
                     GKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTR
                     AEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIR
                     HNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAA
                     GITLGMDELYK"
     primer_bind     complement(6848..6864)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(6894..6912)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(join(9573..9574,1..17))
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
       61 gttctaacga caatatgtcc atatggtgca ctctcagtac aatctgctct gatgccgcat
      121 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
      181 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
      241 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
      301 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
      361 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      421 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      481 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      541 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      601 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      661 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      721 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      781 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      841 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
      901 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
      961 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     1021 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1081 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1141 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     1201 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1261 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1321 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1381 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1441 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1501 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1561 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1621 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     1681 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1741 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1801 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1861 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga
     1921 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     1981 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2041 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2101 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2161 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2221 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     2281 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg caggttaacc tggcttatcg
     2341 aaattaatac gactcactat agggagaccg gcagatccaa tgcggatgga ttcgctcact
     2401 cggtgccgcg gcggtgctaa gttcctgccg tggattgggg gattgcacag acggtggagt
     2461 gcttgctaca gctaccaggt acccagttgc acaccaaacg gtgcccctgt gcccctgcgt
     2521 ctaggtgtgt cctgtgagca actgcgcccg cgcgcggcac cgggaagctg cgggaacctc
     2581 aatggatgat tggcgccaga tccccggcat cacctcagca catgggcagc ccgagaagga
     2641 atcgaagggt acaagatatt ccagggtttc cgctagcaca acttccaagc gcctctaccg
     2701 ctactccaca cacccacgct gaatggcaca accactgagg cctagcggtc ccagctcccc
     2761 gtaaccacgc agcccagcga ccgaggtcat catgacacta tgacgacaat ctggcgggag
     2821 acggcttggg aaaatcgatg acgggcacct accttgggaa cgatagagca agtccagtca
     2881 acgccctagg gaaggggaaa aaaaaaaaaa gagcccatgc tcacaaggag caacacccca
     2941 ctgtgctgct atcatagtac actacaagca aagtcgggat gttgctagaa tccgtcggct
     3001 acgtcggtag atacctccca taggcagtaa gtctaggtat ccaatttata gttccgcgta
     3061 tttcttcgtc gaacggggca cagcaaagcc taacagccgc agtgcctcgc aaccaacacc
     3121 aagcagtaat ctcatccatc catacagatc ctcccacaat atctttacat gcgcacttgc
     3181 gctgtgttag cagtttaggt tccactacca aggctgacac gagcagcact gacagcaaac
     3241 tgctgcttgg ggccaagaaa ttggcagttt gctgagcacc tacatagcag tacctccctt
     3301 accatatttg cttgcttcca cgtccctatc ctcaagcgat tttattcttg actttttgta
     3361 tttcaacgca aaggccattc cggtttctca tgttcaaccc tttggatggc gatgccgctc
     3421 gtcccaggac ctcgtgacct ttgaaagacg agaaatatat cccaaagcaa cttctgctta
     3481 tgggcaagca actgcatggc ggcctacaac tcatatctcg cgtaatcatc caaacgctag
     3541 gcccgagtac tctactgttc tcgagacacc taccatgata tgtacccaat cagacatgac
     3601 aacagtcaac caaacatacc ctgatccggc caaaccctga catccctaga tgatggttaa
     3661 ggcacacaaa tggaaaacgg gactcgtcgg ttaggtggga acgcttgtag tgtagtgtag
     3721 ccagccaacc gactctcctc gtccctctca agtccagtcc cgttattgcc gacccaaccg
     3781 cccagccgcc agctgaggga gccaatggtc taatacaccg tccaacggcc ccagtgatgt
     3841 ttcaaacttg attgacagcg aacgaaacac ctgaaaccca ttatagatat gtatagattt
     3901 gacggggtag cttggcccgg ggctacacta gtagtgtatg taactccatg ccaaacagtc
     3961 ggacgagggt accgcggccg ctagaaggag cagtccatct gcgtgaatca cgagagaatc
     4021 agctactttg aatcgatgga tgcagctacc agaagtcact cagttcgttc aaagccacat
     4081 cactgggcac ttccattggg acaggcattg atcggacgag accgacttct ggccgctttc
     4141 aacagccaca ttatatccat gtcacggcta cgcgtcggcc ttcggtaaca gaaaagcaca
     4201 cagacagcga ttgtgacatg gattcgggca aagcattggt ggtcgcacca ggtcatcctg
     4261 agtgtgcagt ggctgctatt cagatttcat ctaactgcgg gagaggggtt caaaggggcg
     4321 tgacgtcaca gacaacgggt gaaggacgaa gattgcctca cttctttgct agcaatttgc
     4381 ctgcaaagaa ggcgcacatg acaagcaaac aactgggaaa ccactattga atacccacaa
     4441 tgcaaagctc ggaaggtacg tcttgattgc agtgtgtcga gtgtcaaaaa agaagcaagt
     4501 gttcatgcaa gccaaaattg gcacctcctc cacttctccg agtgccccac ccgaacctcc
     4561 aggcgagatg gccggaacat accatccgcg ttgggattat gacgtatctc cttcttcttc
     4621 acatgattcc atcccgttgt tgcttgtttg cgagctgtga cgggagatcg tagatgccac
     4681 ttcgggccag gcaggcagtg caggcagcca ggaacacaag cttccaactt ggtcatctcg
     4741 attgccgatt aagggaacca aatgcctata taagactgtc ctcccacctc cccaatacca
     4801 ttcttttctt cttccatcat cagccaacaa agcaatcaca tcttcactac ttcaaatcaa
     4861 cacaacactc aaaccacttt cacaacccct cacatcaacc aaatctagaa ctagtggatc
     4921 caccatgcct cccaagaaga ccgagaccaa ggccgccgat gcctctgccg ccgccgctcc
     4981 tgctcccgcc gctgctccca cctctgctcc caagaccaag agccccagca cccatgcctc
     5041 ttaccttgta agctttactt aatccccaag agaaacgcat cttttgctaa cattcaaaca
     5101 ggacatgatc acggacgcta ttgttgcggt atttaaaccc cctcattatc tttgttgtgg
     5161 cgtgttttgg ttgtgggcgc ttctctggtc ggtgagcaaa atgagacaat tgctctcatg
     5221 atgcacgcaa ccaagagtca cccacaccac tcaccaccct tttactgtcc ctcagtcgtg
     5281 ctgattcctt ctttttgtca tgctacagct caaggaccgc gctggatcta ggtatgtgcc
     5341 atttttctgc ttacttttct cattttttca ccagggcttt gccaagccac accgcaaacc
     5401 ttgcgggccc ttcctacggt cgcaccgatg accactttca ttcttatttc catgacgcga
     5461 tgaaggatca caacatgttg ctaacgcaca ccacagccgt caagctctca agaagtatgt
     5521 ccgtgccaac aacacgctcg gcaacgtcac cgacaacatg ttcgactcgc tcttcaacaa
     5581 ggccttgaag aacggtgtcg acaagggtgt ctttgagcag cccaagggta tgtcaccacc
     5641 cacatgacgc atcatctcct tgtattactc tttgcatgct cacacacgat ataggtcctt
     5701 ccggtggcac caagcttgcc aagaaggtcg ctaagcctgc tcccaagaag gctgctccca
     5761 agaaggagac caaggagaag aagcccgctg ccgctaagaa ggagggtgcc gccaagaagg
     5821 agaccaagga gaagaaggct cctgctgcca agaaggcggc tgcccccaag aaggctgctg
     5881 ctcccaagaa ggaggtcaag gagaagaagg cggctgcccc caagaagaag gccgctgctc
     5941 ctgccgttgc cgacaaggag accgtcctca ccaagaccaa gtctggccgt gtcgccaaga
     6001 gcaccgctaa gcctgctgcc gccaagaagg ccgctgctcc caagaaggct gccgccagca
     6061 agaaggccga gaaggccgag cccgctgccg agaaggcatt aattaacatg gtgagcaagg
     6121 gctaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     6181 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     6241 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct
     6301 tcacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     6361 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     6421 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     6481 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     6541 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     6601 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     6661 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     6721 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     6781 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaagaa ttcgatatca
     6841 agcttatcga taccgtcgac ctcgaggggg ggcccggtac ccagcttttg ttccctttag
     6901 tgagggttaa ttagggcgtg gacggctaat ggggtctgaa tgctaaaaga caccatttcc
     6961 cacactccct ctgatttttt ttatggtttt tttttatctt catttttcgc cgtcttttat
     7021 tcggttgttt gggaggttca catgcaacgt gcaagactgc aagttttttg agatgggcgc
     7081 ttcatgcaat gcagcatcac tgcagggaca ttgtgacatc cacgtttcga tcgtctgtgc
     7141 ttcccccgtt catgtacgca tgatctgaaa aaaaaaaaaa aaaaaaaggt tgatgtaata
     7201 atgaaaataa acacccaggg acgagcacct catctgctgg aatagggaga aaaactcaag
     7261 ggccgtttgg gtctccacac ttaccaacag attcatgtac tgatcttcag cctgtacaga
     7321 acagtacaat gtccaaaact cgttatcatt actctatgtg cccaaaatga cccagatata
     7381 catcaaccca ctacatcctt ttgttcttgt tttttttttt tttttttgct acttcgaaaa
     7441 gaatttccgt ttagttgctc ttgctcatgt gctcaagacg gatgctgacc gcccttctgt
     7501 gagcctcgag ctcctcaacc ttagccaact gcatgacagc ctggccgacg tttttgagac
     7561 cctcggcagt caagttggag ctggtaatgt gcttgacgaa cgaggcgaaa ttgacgccag
     7621 agtactgctt gccaaagcca taggtagcta tgtgtcttga cgttagcaga agaatctgtg
     7681 aagcggggct atttatgata tgtaacttac gcagcgagtg gttaacacca gcagagtaat
     7741 cgccaacgga ctcaggagtc caggcgccaa tgaagacact accggcgttc atgacaagat
     7801 cgacagcctt ctcggcctcc ttgatctgga ggatcaagtg ctcaggagcg tacttgttgc
     7861 tgagctccat ggcctcctcg acggtcttga cctgcacggt gatcgagtgg gcgatggagc
     7921 cacggacaat ctggacgcga ggaagctccg tagcctgacg gtgaacctcg tcctcaatag
     7981 cctgaagatg ctcctcgtcg aggtcaatag cgatcaggat gacctgactg tcaacgccgt
     8041 gctcagcctg ggacaggaga tccgaggcaa cgaacgcggg gttggcgtcc ttgtcagcga
     8101 tgaccagcac ctcggacggg ccagcgggca tgtcaatacc aacggcagcg ttggtgtcgt
     8161 tgctgacgaa catcttggca gcagtgacga actggttacc ggggccgaga atcttgtcga
     8221 ccttggtgat gctctcggtg ccgtaggcca tggcagctac ggcctgggca ccgccggcaa
     8281 gcacgatgga ctcggcccca accttgtgag cgacgtggac aatctcggga gtgatggttc
     8341 cgtcggcgcg gggaggagag gcgaacacaa tcttgttgca gccggcgacc atggcgggaa
     8401 cacccagcat aagggcagtg ctggggagaa cggcggtacc gccggggatg tagcagccga
     8461 cggcctcgat gggacgagag aaacggctgc agacaacacc gggcatggtc tcgacctgga
     8521 ggggcttctc ctccttctgg gcggcgtgga acttgcggat gttctcgaag gacacgtcga
     8581 tggcagcaat ggtctcctca gggagctgca taagctcctt ggggaagggc gccttcagga
     8641 cggggctagt aagagaggta gccttctcga acttgtgagt gtacgacaga acagccttgt
     8701 cgccgttctt gcggacgtcc tcgatgatgg ggacaatgat cttgtagatg gcatcggacg
     8761 acttttgcgc aggacgcttg agagcagcat cgagctcctc ggtagagacc ttggaggcgt
     8821 cgaaacgtct catggtgatc ttctcagggg tggtctcctg ggcggcctcc ttggtgacag
     8881 gggccgaagt ggcagcgaga gcggacgccg caggcttgat gccctccttc tcagcccact
     8941 tacccttagc atctccagtc ctgcgcttga ccttccagct cttggcgtca aggctccttt
     9001 cgatatcggc aagagtaacg ccggcagcaa cggccctggt aagagcaaag tagaagagat
     9061 cggcagcctc aaaggcgatt tcctgggggg tctgagcggt gcagagctcc tcagcctcct
     9121 ccatgatctt ggcccggact agcttctcat cggagaagag acgggcagtg taggagccct
     9181 cgggggcaga ctgtttcctc gaaatcaaag tctgctcgag cttgggaagg cctttgagct
     9241 gaccaaagca gccggactga tcgaggtggc agaaacgacc cttctgcttc acgacaaact
     9301 tgagagcatc gttatcgcag tcaagcgaga tgcggacgag ctcctgagtg tctccggaag
     9361 tagcaccctt gtaccagaga ccgcgcttcc ggctctgata gacaccagtc tgtgtcctga
     9421 gggcctcgtt cacactctcg gcactgctgt agaccagacc cagagcagtg tcgtgctcat
     9481 caacgacaac ggtggggaga agaccatcgg gacggtcaga cttccagacg ctcgagagga
     9541 tggtggagat ggcaagcttc agctgctcga gttc
//
