LOCUS       62056_11505        4297 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4297
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        183..211
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    219..235
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        600..704
                     /label=AmpR promoter
     CDS             705..1562
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1736..2324
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2568..2645
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             2646..3725
                     /codon_start=1
                     /label=lacI
                     /note="lac repressor"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     protein_bind    3741..3762
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3777..3807
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     CDS             3851..4024
                     /codon_start=1
                     /label=lacZ-alpha
                     /note="LacZ-alpha fragment of beta-galactosidase"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGE"
ORIGIN
        1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
       61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
      121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
      181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
      241 cacaggaaac agtattcgga tccccggaat tcccgggtcg actcgagcgg ccgcatcgtg
      301 actgactgac gatctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
      361 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
      421 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg
      481 atagcggagt gtataattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
      541 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
      601 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
      661 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
      721 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
      781 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
      841 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
      901 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
      961 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     1021 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     1081 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     1141 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     1201 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa
     1261 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     1321 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     1381 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     1441 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     1501 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     1561 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     1621 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     1681 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     1741 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     1801 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     1861 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     1921 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     1981 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     2041 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     2101 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     2161 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     2221 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     2281 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     2341 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     2401 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     2461 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
     2521 attttctcct tacgcatctg tgcggtattt cacaccgcat aaattccgac accatcgaat
     2581 ggtgcaaaac ctttcgcggt atggcatgat agcgcccgga agagagtcaa ttcagggtgg
     2641 tgaatgtgaa accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga
     2701 ccgtttcccg cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg
     2761 aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca
     2821 aacagtcgtt gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa
     2881 ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg
     2941 tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg
     3001 tcagtgggct gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg
     3061 cctgcactaa tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta
     3121 ttattttctc ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc
     3181 accagcaaat cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg
     3241 ctggctggca taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg
     3301 actggagtgc catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc
     3361 ccactgcgat gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg
     3421 agtccgggct gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca
     3481 gctcatgtta tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa
     3541 ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt
     3601 tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc
     3661 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     3721 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     3781 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     3841 ggaaacagct atgaccatga ttacggattc actggccgtc gttttacaac gtcgtgactg
     3901 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     3961 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     4021 cgaatggcgc tttgcctggt ttccggcacc agaagcggtg ccggaaagct ggctggagtg
     4081 cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc acggttacga
     4141 tgcgcccatc tacaccaacg taacctatcc cattacggtc aatccgccgt ttgttcccac
     4201 ggagaatccg acgggttgtt actcgctcac atttaatgtt gatgaaagct ggctacagga
     4261 aggccagacg cgaattattt ttgatggcgt tggaatt
//
