LOCUS       V013120                 4786 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013120
VERSION     V013120
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4786
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        183..211
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    219..235
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             273..767
                     /gene="ftnA"
                     /label="Bacterial non-heme ferritin"
                     /note="Bacterial non-heme ferritin from Escherichia coli
                     (strain K12). Accession#: P0A998"
     promoter        1089..1193
                     /label="AmpR promoter"
     CDS             1194..2051
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      2225..2813
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        3057..3134
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             3135..4214
                     /label="lacI"
                     /note="lac repressor"
     protein_bind    4230..4251
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4266..4296
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     CDS             4340..4513
                     /label="lacZ-alpha"
                     /note="LacZ-alpha fragment of beta-galactosidase"
ORIGIN
        1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
       61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
      121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
      181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
      241 cacaggaaac agtattcgga tccccggaat tcatgctgaa accggaaatg atcgaaaaac
      301 tgaacgaaca gatgaacctg gaactgtact cttctctgct gtaccagcag atgtctgctt
      361 ggtgctctta ccacaccttc gaaggtgctg ctgctttcct gcgtcgtcac gctcaggaag
      421 aaatgaccca catgcagcgt ctgttcgact acctgaccga caccggtaac ctgccgcgta
      481 tcaacaccgt tgaatctccg ttcgctgaat actcttctct ggacgaactg ttccaggaaa
      541 cctacaaaca cgaacagctg atcacccaga aaatcaacga actggctcac gctgctatga
      601 ccaaccagga ctacccgacc ttcaacttcc tgcagtggta cgtttctgaa cagcacgaag
      661 aagaaaaact gttcaaatct atcatcgaca aactgtctct ggctggtaaa tctggtgaag
      721 gtctgtactt catcgacaaa gaactgtcta ccctggacac ccagaactga ctcgagcggc
      781 cgcatcgtga ctgactgacg atctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
      841 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
      901 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
      961 cacgtagcga tagcggagtg tataattctt gaagacgaaa gggcctcgtg atacgcctat
     1021 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     1081 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     1141 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     1201 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     1261 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     1321 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     1381 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
     1441 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1501 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     1561 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1621 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     1681 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag
     1741 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     1801 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     1861 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     1921 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     1981 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     2041 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     2101 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     2161 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2221 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2281 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2341 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     2401 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2461 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2521 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2581 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2641 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     2701 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     2761 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     2821 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     2881 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     2941 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3001 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata aattccgaca
     3061 ccatcgaatg gtgcaaaacc tttcgcggta tggcatgata gcgcccggaa gagagtcaat
     3121 tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct
     3181 cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg
     3241 aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac
     3301 tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc
     3361 cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg
     3421 tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg
     3481 cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg
     3541 tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca
     3601 tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg
     3661 cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc
     3721 tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac
     3781 gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg
     3841 gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg
     3901 ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata
     3961 ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc
     4021 tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca
     4081 atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa
     4141 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     4201 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     4261 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     4321 tttcacacag gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttacaacg
     4381 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     4441 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     4501 cctgaatggc gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg
     4561 gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca
     4621 cggttacgat gcgcccatct acaccaacgt aacctatccc attacggtca atccgccgtt
     4681 tgttcccacg gagaatccga cgggttgtta ctcgctcaca tttaatgttg atgaaagctg
     4741 gctacaggaa ggccagacgc gaattatttt tgatggcgtt ggaatt
//
