LOCUS       V013121                 4804 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013121
VERSION     V013121
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4804
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        183..211
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    219..235
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             267..284
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             291..785
                     /gene="ftnA"
                     /label="Bacterial non-heme ferritin"
                     /note="Bacterial non-heme ferritin from Escherichia coli
                     (strain K12). Accession#: P0A998"
     promoter        1107..1211
                     /label="AmpR promoter"
     CDS             1212..2069
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      2243..2831
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        3075..3152
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             3153..4232
                     /label="lacI"
                     /note="lac repressor"
     protein_bind    4248..4269
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4284..4314
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     CDS             4358..4531
                     /label="lacZ-alpha"
                     /note="LacZ-alpha fragment of beta-galactosidase"
ORIGIN
        1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
       61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
      121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
      181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
      241 cacaggaaac agtattcgga tccatgcacc accaccacca ccacgaattc atgctgaaac
      301 cggaaatgat cgaaaaactg aacgaacaga tgaacctgga actgtactct tctctgctgt
      361 accagcagat gtctgcttgg tgctcttacc acaccttcga aggtgctgct gctttcctgc
      421 gtcgtcacgc tcaggaagaa atgacccaca tgcagcgtct gttcgactac ctgaccgaca
      481 ccggtaacct gccgcgtatc aacaccgttg aatctccgtt cgctgaatac tcttctctgg
      541 acgaactgtt ccaggaaacc tacaaacacg aacagctgat cacccagaaa atcaacgaac
      601 tggctcacgc tgctatgacc aaccaggact acccgacctt caacttcctg cagtggtacg
      661 tttctgaaca gcacgaagaa gaaaaactgt tcaaatctat catcgacaaa ctgtctctgg
      721 ctggtaaatc tggtgaaggt ctgtacttca tcgacaaaga actgtctacc ctggacaccc
      781 agaactgact cgagcggccg catcgtgact gactgacgat ctgcctcgcg cgtttcggtg
      841 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
      901 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
      961 gcgcagccat gacccagtca cgtagcgata gcggagtgta taattcttga agacgaaagg
     1021 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     1081 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     1141 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     1201 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     1261 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     1321 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     1381 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     1441 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     1501 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     1561 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     1621 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     1681 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     1741 acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     1801 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     1861 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     1921 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     1981 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     2041 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     2101 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     2161 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     2221 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     2281 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     2341 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     2401 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     2461 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     2521 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     2581 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     2641 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     2701 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     2761 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     2821 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     2881 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     2941 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     3001 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     3061 accgcataaa ttccgacacc atcgaatggt gcaaaacctt tcgcggtatg gcatgatagc
     3121 gcccggaaga gagtcaattc agggtggtga atgtgaaacc agtaacgtta tacgatgtcg
     3181 cagagtatgc cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg
     3241 tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca
     3301 accgcgtggc acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca
     3361 gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac
     3421 tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg
     3481 cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg
     3541 accaggatgc cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg
     3601 tctctgacca gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg
     3661 gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa
     3721 gttctgtctc ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa
     3781 ttcagccgat agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca
     3841 tgcaaatgct gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg
     3901 cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg
     3961 tagtgggata cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca
     4021 aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg
     4081 gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc
     4141 tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     4201 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     4261 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     4321 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cggattcact
     4381 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     4441 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     4501 ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc cggcaccaga
     4561 agcggtgccg gaaagctggc tggagtgcga tcttcctgag gccgatactg tcgtcgtccc
     4621 ctcaaactgg cagatgcacg gttacgatgc gcccatctac accaacgtaa cctatcccat
     4681 tacggtcaat ccgccgtttg ttcccacgga gaatccgacg ggttgttact cgctcacatt
     4741 taatgttgat gaaagctggc tacaggaagg ccagacgcga attatttttg atggcgttgg
     4801 aatt
//
