LOCUS       40924_21489        4582 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGEX-empty_TetR, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Zhang Y, Ptacin JL, Fisher EC, Caffaro CE, San Jose K, Feldman AW, 
            Turner CR, Romesberg FE.
  TITLE     A semi-synthetic organism that stores and retrieves increased 
            genetic information
  JOURNAL   Nature (2017) In press
REFERENCE   2  (bases 1 to 4582)
  AUTHORS   Zhang Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2017) Department of Chemistry, The Scripps 
            Research Institute, 10550 N Torrey Pines Rd, BCC526, San Diego, CA 
            92037, USA
REFERENCE   3  (bases 1 to 4582)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4582)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature 
            (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-SEP-2017) Department of Chemistry, The Scripps Research 
            Institute, 10550 N Torrey Pines Rd, BCC526, San Diego, CA 92037, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4582
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        400..504
                     /label=AmpR promoter
     CDS             505..1362
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1536..2124
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             2363..2983
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline repressor TetR"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     terminator      complement(3008..3094)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     promoter        3410..3487
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             3488..4567
                     /codon_start=1
                     /label=lacI
                     /note="lac repressor"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 gtaaatcact gcataattcg tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt
       61 gcgccgacat cataacggtt ctggcaaata attgtcgact gatgactgac gatctgcctc
      121 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
      181 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
      241 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtataattct
      301 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
      361 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
      421 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
      481 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
      541 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
      601 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
      661 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
      721 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
      781 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
      841 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
      901 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
      961 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     1021 aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta
     1081 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     1141 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     1201 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     1261 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     1321 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     1381 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     1441 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     1501 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     1561 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     1621 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     1681 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     1741 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     1801 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     1861 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     1921 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     1981 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     2041 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     2101 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     2161 ttttgctggc cttttgctca catgttcttt cctgcgttat ccccgagctc ttagcgcgaa
     2221 ttgtcgaggg aaattttttc taaatacatt caaatatgta tccgctcatg agacaataac
     2281 cctgataaat gcttcaataa tattaaatat ggctggttct cgcagaaaga aacatatcca
     2341 tgaaatcccg ccccgaattg atatgtccag attagataaa agtaaagtga ttaacagcgc
     2401 attagagctg cttaatgagg tcggaatcga aggtttaaca acccgtaaac tcgcccagaa
     2461 gctaggtgta gagcagccta cattgtattg gcatgtaaaa aataagcggg ctttgctcga
     2521 cgccttagcc attgagatgt tagataggca ccatactcac ttttgccctt tagaagggga
     2581 aagctggcaa gattttttac gtaataacgc taaaagtttt agatgtgctt tactaagtca
     2641 tcgcgatgga gcaaaagtac atttaggtac acggcctaca gaaaaacagt atgaaactct
     2701 cgaaaatcaa ttagcctttt tatgccaaca aggtttttca ctagagaatg cattatatgc
     2761 actcagcgct gtggggcatt ttactttagg ttgcgtattg gaagatcaag agcatcaagt
     2821 cgctaaagaa gaaagggaaa cacctactac tgatagtatg ccgccattat tacgacaagc
     2881 tatcgaatta tttgatcacc aaggtgcaga gccagccttc ttattcggcc ttgaattgat
     2941 catttgcgga ttagaaaaac aacttaaatg tgaaagtggg tcttaagcac taggtctagg
     3001 gcggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
     3061 cccagtcttt cgactgagcc tttcgtttta tttgatgcct ctagcacgcg tagagctaga
     3121 gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactaacat gagaattaca
     3181 acttatatcg tatggggctg acttcaggtg ctacatttga agagataaat tgcactgaaa
     3241 tctagatgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     3301 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     3361 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc ataaattccg acaccatcga
     3421 atggtgcaaa acctttcgcg gtatggcatg atagcgcccg gaagagagtc aattcagggt
     3481 ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctcttatca
     3541 gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt
     3601 ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg
     3661 caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca
     3721 aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat
     3781 ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg
     3841 cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc
     3901 tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag
     3961 tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg
     4021 tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct
     4081 ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg
     4141 cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt
     4201 tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac
     4261 cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga
     4321 cagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca
     4381 aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct
     4441 gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc
     4501 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4561 cgggcagtga gcgcaacgca at
//
