LOCUS       V013126                 5257 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013126
VERSION     V013126
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5257
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        183..211
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    219..235
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             705..722
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             744..1238
                     /gene="ftnA"
                     /label="Bacterial non-heme ferritin"
                     /note="Bacterial non-heme ferritin from Escherichia coli
                     (strain K12). Accession#: P0A998"
     promoter        1560..1664
                     /label="AmpR promoter"
     CDS             1665..2522
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      2696..3284
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        3528..3605
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             3606..4685
                     /label="lacI"
                     /note="lac repressor"
     protein_bind    4701..4722
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4737..4767
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     CDS             4811..4984
                     /label="lacZ-alpha"
                     /note="LacZ-alpha fragment of beta-galactosidase"
ORIGIN
        1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
       61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
      121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
      181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
      241 cacaggaaac agtattcgga tccccggaat tcatgttctg catcggtcgt ctgggtggtg
      301 gtggttctca ggttcagctg caggaatctg gtggtggtct ggttcagccg ggtggttctc
      361 tgcgtctgtc ttgcgctgct tctggtcgta ccttctcttc ttacgctatg ggttggttcc
      421 gtcaggctcc gggtaaacag cgtgaattcg ttgctgctat ccgttggtct ggtggttaca
      481 cctactacac cgactctgtt aaaggtcgtt tcaccatctc tcgtgacaac gctaaaacca
      541 ccgtttacct gcagatgaac tctctgaaac cggaagacac cgctgtttac tactgcgctg
      601 ctacctacct gtcttctgac tactctcgtt acgctctgcc gcagcgtccg ctggactacg
      661 actactgggg tcagggtacc caggttaccg tttcttctct ggaacaccac caccaccacc
      721 acgctagcgg agcatccgca ggaatgctga aaccggaaat gatcgaaaaa ctgaacgaac
      781 agatgaacct ggaactgtac tcttctctgc tgtaccagca gatgtctgct tggtgctctt
      841 accacacctt cgaaggtgct gctgctttcc tgcgtcgtca cgctcaggaa gaaatgaccc
      901 acatgcagcg tctgttcgac tacctgaccg acaccggtaa cctgccgcgt atcaacaccg
      961 ttgaatctcc gttcgctgaa tactcttctc tggacgaact gttccaggaa acctacaaac
     1021 acgaacagct gatcacccag aaaatcaacg aactggctca cgctgctatg accaaccagg
     1081 actacccgac cttcaacttc ctgcagtggt acgtttctga acagcacgaa gaagaaaaac
     1141 tgttcaaatc tatcatcgac aaactgtctc tggctggtaa atctggtgaa ggtctgtact
     1201 tcatcgacaa agaactgtct accctggaca cccagaactg actcgagcgg ccgcatcgtg
     1261 actgactgac gatctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     1321 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     1381 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg
     1441 atagcggagt gtataattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
     1501 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     1561 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     1621 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     1681 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     1741 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     1801 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     1861 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
     1921 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     1981 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     2041 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     2101 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     2161 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa
     2221 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     2281 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     2341 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     2401 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     2461 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     2521 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     2581 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     2641 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     2701 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     2761 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     2821 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     2881 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     2941 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     3001 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3061 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     3121 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3181 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     3241 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     3301 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     3361 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     3421 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
     3481 attttctcct tacgcatctg tgcggtattt cacaccgcat aaattccgac accatcgaat
     3541 ggtgcaaaac ctttcgcggt atggcatgat agcgcccgga agagagtcaa ttcagggtgg
     3601 tgaatgtgaa accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga
     3661 ccgtttcccg cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg
     3721 aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca
     3781 aacagtcgtt gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa
     3841 ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg
     3901 tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg
     3961 tcagtgggct gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg
     4021 cctgcactaa tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta
     4081 ttattttctc ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc
     4141 accagcaaat cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg
     4201 ctggctggca taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg
     4261 actggagtgc catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc
     4321 ccactgcgat gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg
     4381 agtccgggct gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca
     4441 gctcatgtta tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa
     4501 ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt
     4561 tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc
     4621 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     4681 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     4741 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     4801 ggaaacagct atgaccatga ttacggattc actggccgtc gttttacaac gtcgtgactg
     4861 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     4921 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     4981 cgaatggcgc tttgcctggt ttccggcacc agaagcggtg ccggaaagct ggctggagtg
     5041 cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc acggttacga
     5101 tgcgcccatc tacaccaacg taacctatcc cattacggtc aatccgccgt ttgttcccac
     5161 ggagaatccg acgggttgtt actcgctcac atttaatgtt gatgaaagct ggctacagga
     5221 aggccagacg cgaattattt ttgatggcgt tggaatt
//
