LOCUS       V006051                 5923 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006051
VERSION     V006051
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5923)
  AUTHORS   Zhang Y, Ptacin JL, Fisher EC, Caffaro CE, San Jose K, Feldman AW,
            Turner CR, Romesberg FE.
  TITLE     A semi-synthetic organism that stores and retrieves increased
            genetic information
  JOURNAL   Nature (2017) In press
REFERENCE   2  (bases 1 to 5923)
  AUTHORS   Zhang Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2017) Department of Chemistry, The Scripps
            Research Institute, 10550 N Torrey Pines Rd, BCC526, San Diego, CA
            92037, USA
REFERENCE   3  (bases 1 to 5923)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5923)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature
            (2017) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-SEP-2017) Department of Chemistry, The Scripps Research
            Institute, 10550 N Torrey Pines Rd, BCC526, San Diego, CA 92037,
            USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        106..134
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    142..158
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             181..1437
                     /gene="pylS"
                     /label="Pyrrolysine--tRNA ligase"
                     /note="Pyrrolysine--tRNA ligase from Methanosarcina
                     barkeri. Accession#: Q6WRH6"
     promoter        1741..1845
                     /label="AmpR promoter"
     CDS             1846..2703
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      2877..3465
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             3704..4324
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     terminator      complement(4349..4435)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     promoter        4751..4828
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             4829..5908
                     /label="lacI"
                     /note="lac repressor"
ORIGIN
        1 gtaaatcact gcataattcg tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt
       61 gcgccgacat cataacggtt ctggcaaata ttctgaaatg agctgttgac aattaatcat
      121 cggctcgtat aatgtgtgga attgtgagcg gataacaatt tcacacagga aacagtattc
      181 atggataaaa aaccgctgga cgttctgatc tccgctacgg gtctgtggat gagccgcacg
      241 ggtacgctgc ataaaattaa acaccacgaa gtgtcacgtt cgaaaatcta tatcgaaatg
      301 gcgtgcggtg atcatctggt ggttaacaat agccgttctt gtcgcaccgc gcgtgccttt
      361 cgccatcaca aataccgcaa aacgtgcaaa cgttgtcgcg tgtcagatga agacattaac
      421 aatttcctga cccgtagtac ggaatccaaa aactcagtga aagttcgcgt cgtgagtgct
      481 ccgaaagtta aaaaagcgat gccgaaaagt gtctcccgtg ccccgaaacc gctggaaaac
      541 tcagtgtcgg caaaagcttc caccaatacg agccgctctg ttccgtcgcc ggcaaaaagc
      601 accccgaaca gctctgtccc ggcaagcgca ccggcaccgt ctctgacgcg tagtcagctg
      661 gatcgcgtgg aagccctgct gtccccggaa gacaaaatct cactgaatat ggcaaaaccg
      721 tttcgtgaac tggaaccgga actggttacc cgtcgcaaaa acgatttcca acgtctgtat
      781 acgaatgatc gcgaagacta cctgggtaaa ctggaacgtg atatcaccaa atttttcgtg
      841 gaccgcggct ttctggaaat caaatctccg attctgatcc cggctgaata tgttgaacgc
      901 atgggtatta acaatgatac cgaactgagt aaacagattt ttcgtgtgga taaaaacctg
      961 tgcctgcggc cgatgctggc accgacgctg tataattacc tgcgtaaact ggatcgcatt
     1021 ctgccgggtc cgattaaaat ctttgaagtg ggcccgtgtt atcgtaaaga atcggatggc
     1081 aaagaacacc tggaagaatt taccatggtt aacttctgcc aaatgggcag cggttgtacg
     1141 cgcgaaaatc tggaagcgct gatcaaagaa ttcctggatt acctggaaat cgacttcgaa
     1201 atcgtcggtg attcttgcat ggtgtatggc gataccctgg acatcatgca tggtgacctg
     1261 gaactgagtt ccgctgttgt cggtccggtc agcctggatc gtgaatgggg cattgacaaa
     1321 ccgtggatcg gcgcgggttt tggcctggaa cgcctgctga aagttatgca cggcttcaaa
     1381 aacatcaaac gtgcgtctcg ctcggaatcg tattacaacg gcatctcaac caatctgtaa
     1441 taatgactga cgatctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     1501 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     1561 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
     1621 gatagcggag tgtataattc ttgaagacga aagggcctcg tgatacgcct atttttatag
     1681 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     1741 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     1801 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     1861 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     1921 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     1981 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     2041 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     2101 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     2161 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     2221 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     2281 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     2341 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca
     2401 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     2461 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     2521 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     2581 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     2641 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     2701 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     2761 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     2821 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     2881 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2941 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     3001 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     3061 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     3121 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     3181 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     3241 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     3301 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     3361 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     3421 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     3481 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     3541 tccccgagct cttagcgcga attgtcgagg gaaatttttt ctaaatacat tcaaatatgt
     3601 atccgctcat gagacaataa ccctgataaa tgcttcaata atattaaata tggctggttc
     3661 tcgcagaaag aaacatatcc atgaaatccc gccccgaatt gatatgtcca gattagataa
     3721 aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg aaggtttaac
     3781 aacccgtaaa ctcgcccaga agctaggtgt agagcagcct acattgtatt ggcatgtaaa
     3841 aaataagcgg gctttgctcg acgccttagc cattgagatg ttagataggc accatactca
     3901 cttttgccct ttagaagggg aaagctggca agatttttta cgtaataacg ctaaaagttt
     3961 tagatgtgct ttactaagtc atcgcgatgg agcaaaagta catttaggta cacggcctac
     4021 agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac aaggtttttc
     4081 actagagaat gcattatatg cactcagcgc tgtggggcat tttactttag gttgcgtatt
     4141 ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa acacctacta ctgatagtat
     4201 gccgccatta ttacgacaag ctatcgaatt atttgatcac caaggtgcag agccagcctt
     4261 cttattcggc cttgaattga tcatttgcgg attagaaaaa caacttaaat gtgaaagtgg
     4321 gtcttaagca ctaggtctag ggcggcggat ttgtcctact caggagagcg ttcaccgaca
     4381 aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc
     4441 tctagcacgc gtagagctag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
     4501 atgactaaca tgagaattac aacttatatc gtatggggct gacttcaggt gctacatttg
     4561 aagagataaa ttgcactgaa atctagatga ttctgtggat aaccgtatta ccgcctttga
     4621 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     4681 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     4741 cataaattcc gacaccatcg aatggtgcaa aacctttcgc ggtatggcat gatagcgccc
     4801 ggaagagagt caattcaggg tggtgaatgt gaaaccagta acgttatacg atgtcgcaga
     4861 gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc
     4921 tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg
     4981 cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct
     5041 ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg
     5101 tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt
     5161 gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca
     5221 ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc
     5281 tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt
     5341 ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc
     5401 tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca
     5461 gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca
     5521 aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct
     5581 gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt
     5641 gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca
     5701 ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca
     5761 ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc
     5821 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     5881 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aat
//
