LOCUS       40924_21479        8369 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pGEX-6P-3-GST-hTERT, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8369)
  AUTHORS   Hansen DT, Thiyagarajan T, Larson AC, Hansen JL.
  TITLE     Telomerase repeat amplification protocol (TRAP) activity upon 
            recombinant expression and purification of human telomerase in a 
            bacterial system
  JOURNAL   Protein Expr. Purif. (2016) In press
  PUBMED    26965413
REFERENCE   2  (bases 1 to 8369)
  AUTHORS   Hansen DT, Hansen JL.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2015) Department of Biochemistry and Molecular 
            Biology, Medical University of South Carolina, 173 Ashley Avenue, 
            Charleston, SC 29425, USA
REFERENCE   3  (bases 1 to 8369)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8369)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr. Purif. (2016) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-DEC-2015) Department of Biochemistry and Molecular Biology, 
            Medical University of South Carolina, 173 Ashley Avenue, Charleston,
            SC 29425, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        183..211
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    219..235
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             258..911
                     /codon_start=1
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             918..941
                     /codon_start=1
                     /label=HRV 3C site
                     /note="recognition and cleavage site for human rhinovirus
                     3C and PreScission proteases"
                     /translation="LEVLFQGP"
     CDS             960..4355
                     /codon_start=1
                     /label=hTERT
                     /note="human telomerase reverse transcriptase, the
                     catalytic subunit of telomerase"
                     /translation="MPRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDPA
                     AFRALVAQCLVCVPWDARPPPAAPSFRQVSCLKELVARVLQRLCERGAKNVLAFGFALL
                     DGARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFVLVA
                     PSCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPAPGAR
                     RRGGSASRSLPLPKRPRRGAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAEE
                     ATSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYSSGDKEQ
                     LRPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPLFLELLGN
                     HAQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQLLRQHSSP
                     WQVYGFVRACLRRLVPPGLWGSRHNERRFLRNTKKFISLGKHAKLSLQELTWKMSVRDC
                     AWLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSFFYVTETTFQKNRLFFY
                     RKSVWSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFIPKPDGLRPIVN
                     MDYVVGARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLGLDDIHRAWRTFV
                     LRVRAQDPPPELYFVKVDVTGAYDTIPQDRLTEVIASIIKPQNTYCVRRYAVVQKAAHG
                     HVRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSLNEASSGLFDVFLRF
                     MCHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAGIRRDGLLLRLVDDFL
                     LVTPHLTHAKTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEALGGTAFVQMPAHGLFPW
                     CGLLLDTRTLEVQSDYSSYARTSIRASLTFNRGFKAGRNMRRKLFGVLRLKCHSLFLDL
                     QVNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPTFFLRVISDTASLCYSILK
                     AKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTAQTQLSRKL
                     PGTTLTALEAAANPALPSDFKTILD"
     promoter        4672..4776
                     /label=AmpR promoter
     CDS             4777..5634
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5808..6396
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        6640..6717
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             6718..7797
                     /codon_start=1
                     /label=lacI
                     /note="lac repressor"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     protein_bind    7813..7834
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7849..7879
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7887..7903
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             7923..8096
                     /codon_start=1
                     /label=lacZ-alpha
                     /note="LacZ-alpha fragment of beta-galactosidase"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGE"
ORIGIN
        1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
       61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
      121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
      181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
      241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
      301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
      361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
      421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
      481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
      541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
      601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
      661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
      721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
      781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
      841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
      901 atcctccaaa atcggatctg gaagttctgt tccaggggcc cctgggatcc ccgaattcta
      961 tgccgcgcgc tccccgctgc cgagccgtgc gctccctgct gcgcagccac taccgcgagg
     1021 tgctgccgct ggccacgttc gtgcggcgcc tggggcccca gggctggcgg ctggtgcagc
     1081 gcggggaccc ggcggctttc cgcgcgctgg tggcccagtg cctggtgtgc gtgccctggg
     1141 acgcacggcc gccccccgcc gccccctcct tccgccaggt gtcctgcctg aaggagctgg
     1201 tggcccgagt gctgcagagg ctgtgcgagc gcggcgcgaa gaacgtgctg gccttcggct
     1261 tcgcgctgct ggacggggcc cgcgggggcc cccccgaggc cttcaccacc agcgtgcgca
     1321 gctacctgcc caacacggtg accgacgcac tgcgggggag cggggcgtgg gggctgctgc
     1381 tgcgccgcgt gggcgacgac gtgctggttc acctgctggc acgctgcgcg ctctttgtgc
     1441 tggtggctcc cagctgcgcc taccaggtgt gcgggccgcc gctgtaccag ctcggcgctg
     1501 ccactcaggc ccggcccccg ccacacgcta gtggaccccg aaggcgtctg ggatgcgaac
     1561 gggcctggaa ccatagcgtc agggaggccg gggtccccct gggcctgcca gccccgggtg
     1621 cgaggaggcg cgggggcagt gccagccgaa gtctgccgtt gcccaagagg cccaggcgtg
     1681 gcgctgcccc tgagccggag cggacgcccg ttgggcaggg gtcctgggcc cacccgggca
     1741 ggacgcgtgg accgagtgac cgtggtttct gtgtggtgtc acctgccaga cccgccgaag
     1801 aagccacctc tttggagggt gcgctctctg gcacgcgcca ctcccaccca tccgtgggcc
     1861 gccagcacca cgcgggcccc ccatccacat cgcggccacc acgtccctgg gacacgcctt
     1921 gtcccccggt gtacgccgag accaagcact tcctctactc ctcaggcgac aaggagcagc
     1981 tgcggccctc cttcctactc agctctctga ggcccagcct gactggcgct cggaggctcg
     2041 tggagaccat ctttctgggt tccaggccct ggatgccagg gactccccgc aggttgcccc
     2101 gcctgcccca gcgctactgg caaatgcggc ccctgtttct ggagctgctt gggaaccacg
     2161 cgcagtgccc ctacggggtg ctcctcaaga cgcactgccc gctgcgagct gcggtcaccc
     2221 cagcagccgg tgtctgtgcc cgggagaagc cccagggctc tgtggcggcc cccgaggagg
     2281 aggacacaga cccccgtcgc ctggtgcagc tgctccgcca gcacagcagc ccctggcagg
     2341 tgtacggctt cgtgcgggcc tgcctgcgcc ggctggtgcc cccaggcctc tggggctcca
     2401 ggcacaacga acgccgcttc ctcaggaaca ccaagaagtt catctccctg gggaagcatg
     2461 ccaagctctc gctgcaggag ctgacgtgga agatgagcgt gcgggactgc gcttggctgc
     2521 gcaggagccc aggggttggc tgtgttccgg ccgcagagca ccgtctgcgt gaggagatcc
     2581 tggccaagtt cctgcactgg ctgatgagtg tgtacgtcgt cgagctgctc aggtctttct
     2641 tttatgtcac ggagaccacg tttcaaaaga acaggctctt tttctaccgg aagagtgtct
     2701 ggagcaagtt gcaaagcatt ggaatcagac agcacttgaa gagggtgcag ctgcgggagc
     2761 tgtcggaagc agaggtcagg cagcatcggg aagccaggcc cgccctgctg acgtccagac
     2821 tccgcttcat ccccaagcct gacgggctgc ggccgattgt gaacatggac tacgtcgtgg
     2881 gagccagaac gttccgcaga gaaaagaggg ccgagcgtct cacctcgagg gtgaaggcac
     2941 tgttcagcgt gctcaactac gagcgggcgc ggcgccccgg cctcctgggc gcctctgtgc
     3001 tgggcctgga cgatatccac agggcctggc gcaccttcgt gctgcgtgtg cgggcccagg
     3061 acccgccgcc tgagctgtac tttgtcaagg tggatgtgac gggcgcgtac gacaccatcc
     3121 cccaggacag gctcacggag gtcatcgcca gcatcatcaa accccagaac acgtactgcg
     3181 tgcgtcggta tgccgtggtc cagaaggccg cccatgggca cgtccgcaag gccttcaaga
     3241 gccacgtctc taccttgaca gacctccagc cgtacatgcg acagttcgtg gctcacctgc
     3301 aggagaccag cccgctgagg gatgccgtcg tcatcgagca gagctcctcc ctgaatgagg
     3361 ccagcagtgg cctcttcgac gtcttcctac gcttcatgtg ccaccacgcc gtgcgcatca
     3421 ggggcaagtc ctacgtccag tgccagggga tcccgcaggg ctccatcctc tccacgctgc
     3481 tctgcagcct gtgctacggc gacatggaga acaagctgtt tgcggggatt cggcgggacg
     3541 ggctgctcct gcgtttggtg gatgatttct tgttggtgac acctcacctc acccacgcga
     3601 aaaccttcct caggaccctg gtccgaggtg tccctgagta tggctgcgtg gtgaacttgc
     3661 ggaagacagt ggtgaacttc cctgtagaag acgaggccct gggtggcacg gcttttgttc
     3721 agatgccggc ccacggccta ttcccctggt gcggcctgct gctggatacc cggaccctgg
     3781 aggtgcagag cgactactcc agctatgccc ggacctccat cagagccagt ctcaccttca
     3841 accgcggctt caaggctggg aggaacatgc gtcgcaaact ctttggggtc ttgcggctga
     3901 agtgtcacag cctgtttctg gatttgcagg tgaacagcct ccagacggtg tgcaccaaca
     3961 tctacaagat cctcctgctg caggcgtaca ggtttcacgc atgtgtgctg cagctcccat
     4021 ttcatcagca agtttggaag aaccccacat ttttcctgcg cgtcatctct gacacggcct
     4081 ccctctgcta ctccatcctg aaagccaaga acgcagggat gtcgctgggg gccaagggcg
     4141 ccgccggccc tctgccctcc gaggccgtgc agtggctgtg ccaccaagca ttcctgctca
     4201 agctgactcg acaccgtgtc acctacgtgc cactcctggg gtcactcagg acagcccaga
     4261 cgcagctgag tcggaagctc ccggggacga cgctgactgc cctggaggcc gcagccaacc
     4321 cggcactgcc ctcagacttc aagaccatcc tggactgagc ggccgcatcg tgactgactg
     4381 acgatctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     4441 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     4501 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
     4561 gtgtataatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     4621 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     4681 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     4741 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     4801 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     4861 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     4921 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     4981 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
     5041 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5101 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5161 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5221 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5281 gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg
     5341 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5401 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     5461 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     5521 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     5581 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     5641 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     5701 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     5761 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     5821 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     5881 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     5941 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6001 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6061 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6121 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6181 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6241 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6301 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6361 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     6421 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     6481 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     6541 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc
     6601 cttacgcatc tgtgcggtat ttcacaccgc ataaattccg acaccatcga atggtgcaaa
     6661 acctttcgcg gtatggcatg atagcgcccg gaagagagtc aattcagggt ggtgaatgtg
     6721 aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc
     6781 cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg
     6841 atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg
     6901 ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg
     6961 gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga
     7021 agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg
     7081 ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact
     7141 aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc
     7201 tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa
     7261 atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg
     7321 cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt
     7381 gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg
     7441 atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg
     7501 ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt
     7561 tatatcccgc cgtcaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg
     7621 gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc
     7681 tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg
     7741 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     7801 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     7861 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     7921 ctatgaccat gattacggat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     7981 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     8041 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
     8101 gctttgcctg gtttccggca ccagaagcgg tgccggaaag ctggctggag tgcgatcttc
     8161 ctgaggccga tactgtcgtc gtcccctcaa actggcagat gcacggttac gatgcgccca
     8221 tctacaccaa cgtaacctat cccattacgg tcaatccgcc gtttgttccc acggagaatc
     8281 cgacgggttg ttactcgctc acatttaatg ttgatgaaag ctggctacag gaaggccaga
     8341 cgcgaattat ttttgatggc gttggaatt
//
