LOCUS       V014184                 5630 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014184
VERSION     V014184
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5630)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        58..86
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    94..110
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             133..786
                     /label="GST"
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             793..810
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
     CDS             811..1326
                     /note="RNA silencing suppressor p19 from Tomato bushy stunt
                     virus (strain Cherry). Accession#: P11690"
                     /label="RNA silencing suppressor p19"
     CDS             1327..1344
                     /label="6xHis"
                     /note="6xHis affinity tag"
     promoter        1348..1366
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        1808..1912
                     /label="AmpR promoter"
     CDS             1913..2770
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      2944..3532
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        3776..3853
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             3854..4933
                     /label="lacI"
                     /note="lac repressor"
     protein_bind    4949..4970
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4985..5015
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     CDS             5059..5232
                     /label="lacZ-alpha"
                     /note="LacZ-alpha fragment of beta-galactosidase"
ORIGIN
        1 ataatgtttt ttgcgccgac atcataacgg ttctggcaaa tattctgaaa tgagctgttg
       61 acaattaatc atcggctcgt ataatgtgtg gaattgtgag cggataacaa tttcacacag
      121 gaaacagtat tcatgtcccc tatactaggt tattggaaaa ttaagggcct tgtgcaaccc
      181 actcgacttc ttttggaata tcttgaagaa aaatatgaag agcatttgta tgagcgcgat
      241 gaaggtgata aatggcgaaa caaaaagttt gaattgggtt tggagtttcc caatcttcct
      301 tattatattg atggtgatgt taaattaaca cagtctatgg ccatcatacg ttatatagct
      361 gacaagcaca acatgttggg tggttgtcca aaagagcgtg cagagatttc aatgcttgaa
      421 ggagcggttt tggatattag atacggtgtt tcgagaattg catatagtaa agactttgaa
      481 actctcaaag ttgattttct tagcaagcta cctgaaatgc tgaaaatgtt cgaagatcgt
      541 ttatgtcata aaacatattt aaatggtgat catgtaaccc atcctgactt catgttgtat
      601 gacgctcttg atgttgtttt atacatggac ccaatgtgcc tggatgcgtt cccaaaatta
      661 gtttgtttta aaaaacgtat tgaagctatc ccacaaattg ataagtactt gaaatccagc
      721 aagtatatag catggccttt gcagggctgg caagccacgt ttggtggtgg cgaccatcct
      781 ccaaaatcgg atctggttcc gcgtggatcc atggaacgag ctatacaagg aaacgacgct
      841 agggaacaag ctaacagtga acgttgggat ggaggatcag gaggtaccac ttctcccttc
      901 aaacttcctg acgaaagtcc gagttggact gagtggcggc tacataacga tgagacgaat
      961 tcgaatcaag ataatcccct tggtttcaag gaaagctggg gtttcgggaa agttgtattt
     1021 aagagatatc tcagatacga caggacggaa gcttcactgc acagagtcct tggatcttgg
     1081 acgggagatt cggttaacta tgcagcatct cgatttttcg gtttcgacca gatcggatgt
     1141 acctatagta ttcggtttcg aggagttagt atcaccgttt ctggagggtc gcgaactctt
     1201 cagcatctct gtgagatggc aattcggtct aagcaagaac tgctacagct tgccccaatc
     1261 gaagtggaaa gtaatgtatc aagaggatgc cctgaaggta ctgagacctt cgaaaaagaa
     1321 agcgagcacc atcaccatca ccattagtaa tacgactcac tatagggagc tcggcctggc
     1381 tggacagagt tgtcatgtgt ctgcctgtct acacttgctg tgcagctcga gaacatccgc
     1441 tcacgtcgac ctgtacagca ggcacagaca ggcagtcaca tgacaaccca gcctgcggcc
     1501 gcatcgtgac tgactgacga tctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
     1561 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     1621 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
     1681 acgtagcgat agcggagtgt ataattcttg aagacgaaag ggcctcgtga tacgcctatt
     1741 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     1801 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     1861 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     1921 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     1981 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     2041 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     2101 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga
     2161 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     2221 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     2281 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     2341 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     2401 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc
     2461 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     2521 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     2581 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     2641 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     2701 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     2761 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     2821 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     2881 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     2941 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3001 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3061 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     3121 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3181 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3241 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3301 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3361 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3421 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     3481 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     3541 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     3601 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     3661 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct
     3721 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcataa attccgacac
     3781 catcgaatgg tgcaaaacct ttcgcggtat ggcatgatag cgcccggaag agagtcaatt
     3841 cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc
     3901 ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga aaacgcggga
     3961 aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg cacaacaact
     4021 ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc agtctggccc tgcacgcgcc
     4081 gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt
     4141 gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc
     4201 gcaacgcgtc agtgggctga tcattaacta tccgctggat gaccaggatg ccattgctgt
     4261 ggaagctgcc tgcactaatg ttccggcgtt atttcttgat gtctctgacc agacacccat
     4321 caacagtatt attttctccc atgaagacgg tacgcgactg ggcgtggagc atctggtcgc
     4381 attgggtcac cagcaaatcg cgctgttagc gggcccatta agttctgtct cggcgcgtct
     4441 gcgtctggct ggctggcata aatatctcac tcgcaatcaa attcagccga tagcggaacg
     4501 ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg
     4561 catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc
     4621 cattaccgag tccgggctgc gcgttggtgc ggatatctcg gtagtgggat acgacgatac
     4681 cgaagacagc tcatgttata tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct
     4741 ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa
     4801 tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac
     4861 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     4921 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     4981 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     5041 ttcacacagg aaacagctat gaccatgatt acggattcac tggccgtcgt tttacaacgt
     5101 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     5161 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     5221 ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg
     5281 ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac
     5341 ggttacgatg cgcccatcta caccaacgta acctatccca ttacggtcaa tccgccgttt
     5401 gttcccacgg agaatccgac gggttgttac tcgctcacat ttaatgttga tgaaagctgg
     5461 ctacaggaag gccagacgcg aattattttt gatggcgttg gaattacgtt atcgactgca
     5521 cggtgcacca atgcttctgg cgtcaggcag ccatcggaag ctgtggtatg gctgtgcagg
     5581 tcgtaaatca ctgcataatt cgtgtcgctc aaggcgcact cccgttctgg
//
