LOCUS       V013985                 5656 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013985
VERSION     V013985
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5656)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        183..211
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    219..235
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             279..296
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             297..809
                     /note="RNA silencing suppressor p19 from Tomato bushy stunt
                     virus (strain Cherry). Accession#: P11690"
                     /label="RNA silencing suppressor p19"
     CDS             815..1492
                     /gene="rnc"
                     /label="Ribonuclease 3"
                     /note="Ribonuclease 3 from Escherichia coli (strain K12 /
                     MC4100 / BW2952). Accession#: C4ZYJ0"
     promoter        1499..1517
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        1959..2063
                     /label="AmpR promoter"
     CDS             2064..2921
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3095..3683
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        3927..4004
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             4005..5084
                     /label="lacI"
                     /note="lac repressor"
     protein_bind    5100..5121
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5136..5166
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     CDS             5210..5383
                     /label="lacZ-alpha"
                     /note="LacZ-alpha fragment of beta-galactosidase"
ORIGIN
        1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
       61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
      121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
      181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
      241 cacaggaaac agtattcgga tccccggaat tccatatgca tcatcaccac catcacgaac
      301 gtgcaatcca gggcaacgac gcccgtgaac aggccaacag cgaacgctgg gatggtggca
      361 gcggcggtac aaccagccct tttaaactgc cggacgaaag cccgagttgg accgaatggc
      421 gcctgcataa cgatgagacc aacagcaacc aggacaaccc gctgggtttt aaagaaagct
      481 ggggcttcgg caaagtggtg ttcaagcgct atctgcgcta cgatcgtacc gaagccagcc
      541 tgcatcgcgt tctgggtagc tggaccggtg acagcgtgaa ttatgccgcc agccgttttt
      601 tcggcttcga tcagatcggc tgcacctaca gcattcgctt ccgcggtgtg agcattaccg
      661 ttagcggcgg tagccgtaca ctgcagcacc tgtgtgaaat ggccattcgc agtaagcaag
      721 agctgctgca gctggccccg attgaggtgg aaagtaatgt gagccgtggc tgtccggagg
      781 gcacccaaac cttcgaaaaa gagagtgagt aatgatgaac cccatcgtaa ttaatcggct
      841 tcaacggaag ctgggctaca cttttaatca tcaggaactg ttgcagcagg cattaactca
      901 tcgtagtgcc agcagtaaac ataacgagcg tttagaattt ttaggcgact ctattctgag
      961 ctacgttatc gccaatgcgc tttatcaccg tttccctcgt gtggatgaag gcgatatgag
     1021 ccggatgcgc gccacgctgg tccgtggcaa tacgctggcg gaactggcgc gcgaatttga
     1081 gttaggcgag tgcttacgtt tagggccagg tgaacttaaa agcggtggat ttcgtcgtga
     1141 gtcaattctc gccgacaccg tcgaagcatt aattggtggc gtattcctcg acagtgatat
     1201 tcaaaccgtc gagaaattaa tcctcaactg gtatcaaact cgtttggacg aaattagccc
     1261 aggcgataaa caaaaagatc cgaaaacgcg cttgcaagaa tatttgcagg gtcgccatct
     1321 gccgctgccg acttatctgg tagtccaggt acgtggcgaa gcgcacgatc aggaatttac
     1381 tatccactgc caggtcagcg gcctgagtga accggtggtt ggcacaggtt caagccgtcg
     1441 taaggctgag caggctgccg ccgaacaggc gttgaaaaaa ctggagctgg aatgaatcta
     1501 atacgactca ctatagggag ctcggcctgg ctggacagag ttgtcatgtg tctgcctgtc
     1561 tacacttgct gtgcagctcg agaacatccg ctcacgtcga cctgtacagc aggcacagac
     1621 aggcagtcac atgacaaccc agcctgcggc cgcatcgtga ctgactgacg atctgcctcg
     1681 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     1741 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     1801 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tataattctt
     1861 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     1921 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     1981 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     2041 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     2101 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     2161 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     2221 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     2281 tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca
     2341 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     2401 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     2461 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     2521 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     2581 acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa
     2641 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     2701 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     2761 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     2821 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     2881 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     2941 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     3001 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     3061 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     3121 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3181 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3241 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3301 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3361 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3421 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3481 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3541 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3601 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3661 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     3721 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     3781 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     3841 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt
     3901 gcggtatttc acaccgcata aattccgaca ccatcgaatg gtgcaaaacc tttcgcggta
     3961 tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt
     4021 tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc
     4081 aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga
     4141 attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg
     4201 ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc
     4261 gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag
     4321 cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact
     4381 atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt
     4441 tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg
     4501 gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag
     4561 cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca
     4621 ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt
     4681 ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca
     4741 acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg
     4801 cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt
     4861 taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc
     4921 aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa
     4981 gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     5041 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     5101 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     5161 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     5221 tacggattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     5281 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     5341 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgct ttgcctggtt
     5401 tccggcacca gaagcggtgc cggaaagctg gctggagtgc gatcttcctg aggccgatac
     5461 tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat gcgcccatct acaccaacgt
     5521 aacctatccc attacggtca atccgccgtt tgttcccacg gagaatccga cgggttgtta
     5581 ctcgctcaca tttaatgttg atgaaagctg gctacaggaa ggccagacgc gaattatttt
     5641 tgatggcgtt ggaatt
//
