LOCUS       V014593                11113 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014593
VERSION     V014593
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11113)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11113
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        887..934
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             1021..2460
                     /label="LuxC"
                     /note="LuxC fatty acid reductase"
     CDS             2476..3396
                     /label="LuxD"
                     /note="LuxD acyltransferase"
     CDS             3448..4527
                     /label="LuxA"
                     /note="LuxA luciferase subunit"
     CDS             4545..5525
                     /label="LuxB"
                     /note="LuxB luciferase subunit"
     CDS             5707..6816
                     /label="LuxE"
                     /note="LuxE"
     rep_origin      complement(7074..7823)
                     /direction=LEFT
                     /label="RSF ori"
                     /note="Plasmids containing the RSF 1030 origin of
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     terminator      complement(7987..8073)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(8791..9447)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(9448..9550)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             join(10470..11113,1..268)
                     /gene="parM"
                     /label="Plasmid segregation protein ParM"
                     /note="Plasmid segregation protein ParM from Escherichia
                     coli. Accession#: P11904"
ORIGIN
        1 taataactat ctgaagcaac gaattaatga tgagaacgaa atatcaatag tcaccgaagc
       61 aatgaatgaa gcacttcgta aacttgagca acgtgtatta aatacgctca atgaattttc
      121 tggttatact catgttatgg ttataggcgg tggcgcagaa ttaatatgcg atgcagtaaa
      181 aaaacacaca cagattcgtg atgaacgttt tttcaaaacc aataactctc aatatgattt
      241 agttaacggt atgtatctca taggtaatta atgatggaca agcgcagaac cattgccttc
      301 aaactaaatc cagatgtaaa tcaaacagat aaaattgttt gtgatacact ggacagtatc
      361 ccgcaagggg aacgaagccg ccttaaccgg gccgcactga cggcaggtct ggccttatac
      421 agacaagatc cccggacccc tttcctttta tgtgagctgc tgacgaaaga aaccacattt
      481 tcagatatcg tgaatatatt gagatcgcta tttccaaaag agatggccga ttttaattct
      541 tcaatagtca ctcaatcctc ttcacaacaa gagcaaaaaa gtgatgaaga gaccaaaaaa
      601 aatgcgatga agctaataaa ttaattcaat tattattgag ttccctttat ccactatcag
      661 gctggataaa gggaactcaa tcaagttatt ttcttaccag tcattacata atcgttatta
      721 tgaaataatc gtttgcactg tctctgttat tcaggcaatt tcaataaagg cacttgctca
      781 cgctctgtca ttttctgaaa ctctcatgtg gaattcctgc agggcatgcc ccgggcatat
      841 gaagcttggt accgggatcc gcggccgcgt ttaaacccat ggacgtgttg acaattaatc
      901 atcggcatag tatatcggca tagtataata cgactcaggg cccactagtg gtacccgggg
      961 atcctctaga gtcgacctgc aggtcgacgg atccggggaa ttcaggcttg gaggatacgt
     1021 atgactaaaa aaatttcatt cattattaac ggccaggttg aaatctttcc cgaaggtgat
     1081 gatttagtgc aatccattaa ttttggtgat aatagtgttt acctgccaat attgaatgac
     1141 tctcatgtaa aaaacattat tgattgtaat ggaaataacg aattacggtt gcataacatt
     1201 gtcaattttc tctatacggt agggcaaaga tggaaaaatg aagaatactc aagacgcagg
     1261 acatacattc gtgacttaaa aaaatatatg ggatattcag aagaaatggc taagctagag
     1321 gccaattgga tatctatgat tttatgttct aaaggcggcc tttatgatgt tgtagaaaat
     1381 gaacttggtt ctcgccatat catggatgaa tggctacctc aggatgaaag ttatgttcgg
     1441 gcttttccga aaggtaaatc tgtacatctg ttggcaggta atgttccatt atctgggatc
     1501 atgtctatat tacgcgcaat tttaactaag aatcagtgta ttataaaaac atcgtcaacc
     1561 gatcctttta ccgctaatgc attagcgtta agttttattg atgtagaccc taatcatccg
     1621 ataacgcgct ctttatctgt tatatattgg ccccaccaag gtgatacatc actcgcaaaa
     1681 gaaattatgc aacatgcgga tgttattgtc gcttggggag ggccagatgc gattaattgg
     1741 gcggtagagc atgcgccatc ttatgctgat gtgattaaat ttggttctaa aaagagtctt
     1801 tgcattatcg ataatcctgt tgatttgacg tccgcagcga caggtgcggc tcatgatgtt
     1861 tgtttttacg atcagcgagc ttgtttttct gcccaaaaca tatattacat gggaaatcat
     1921 tatgaggaat ttaagttagc gttgatagaa aaacttaatc tatatgcgca tatattaccg
     1981 aatgccaaaa aagattttga tgaaaaggcg gcctattctt tagttcaaaa agaaagcttg
     2041 tttgctggat taaaagtaga ggtggatatt catcaacgtt ggatgattat tgagtcaaat
     2101 gcaggtgtgg aatttaatca accacttggc agatgtgtgt accttcatca cgtcgataat
     2161 attgagcaaa tattgcctta tgttcaaaaa aataagacgc aaaccatatc tatttttcct
     2221 tgggagtcat catttaaata tcgagatgcg ttagcattaa aaggtgcgga aaggattgta
     2281 gaagcaggaa tgaataacat atttcgagtt ggtggatctc atgacggaat gagaccgttg
     2341 caacgattag tgacatatat ttctcatgaa aggccatcta actatacggc taaggatgtt
     2401 gcggttgaaa tagaacagac tcgattcctg gaagaagata agttccttgt atttgtccca
     2461 taataggtaa aaagtatgga aaatgaatca aaatataaaa ccatcgacca cgttatttgt
     2521 gttgaaggaa ataaaaaaat tcatgtttgg gaaacgctgc cagaagaaaa cagcccaaag
     2581 agaaagaatg ccattattat tgcgtctggt tttgcccgca ggatggatca ttttgctggt
     2641 ctggcggaat atttatcgcg gaatggattt catgtgatcc gctatgattc gcttcaccac
     2701 gttggattga gttcagggac aattgatgaa tttacaatgt ctataggaaa gcagagcttg
     2761 ttagcagtgg ttgattggtt aactacacga aaaataaata acttcggtat gttggcttca
     2821 agcttatctg cgcggatagc ttatgcaagc ctatctgaaa tcaatgcttc gtttttaatc
     2881 accgcagtcg gtgttgttaa cttaagatat tctcttgaaa gagctttagg gtttgattat
     2941 ctcagtctac ccattaatga attgccgaat aatctagatt ttgaaggcca taaattgggt
     3001 gctgaagtct ttgcgagaga ttgtcttgat tttggttggg aagatttagc ttctacaatt
     3061 aataacatga tgtatcttga tataccgttt attgctttta ctgcaaataa cgataattgg
     3121 gtcaagcaag atgaagttat cacattgtta tcaaatattc gtagtaatcg atgcaagata
     3181 tattctttgt taggaagttc gcatgacttg agtgaaaatt tagtggtcct gcgcaatttt
     3241 tatcaatcgg ttacgaaagc cgctatcgcg atggataatg atcatctgga tattgatgtt
     3301 gatattactg aaccgtcatt tgaacattta actattgcga cagtcaatga acgccgaatg
     3361 agaattgaga ttgaaaatca agcaatttct ctgtcttaaa atctattgag atattctatc
     3421 actcaaatag caatataagg actctctatg aaatttggaa actttttgct tacataccaa
     3481 cctccccaat tttctcaaac agaggtaatg aaacgtttgg ttaaattagg tcgcatctct
     3541 gaggagtgtg gttttgatac cgtatggtta ctggagcatc atttcacgga gtttggtttg
     3601 cttggtaacc cttatgtcgc tgctgcatat ttacttggcg cgactaaaaa attgaatgta
     3661 ggaactgccg ctattgttct tcccacagcc catccagtac gccaacttga agatgtgaat
     3721 ttattggatc aaatgtcaaa aggacgattt cggtttggta tttgccgagg gctttacaac
     3781 aaggactttc gcgtattcgg cacagatatg aataacagtc gcgccttagc ggaatgctgg
     3841 tacgggctga taaagaatgg catgacagag ggatatatgg aagctgataa tgaacatatc
     3901 aagttccata aggtaaaagt aaaccccgcg gcgtatagca gaggtggcgc accggtttat
     3961 gtggtggctg aatcagcttc gacgactgag tgggctgctc aatttggcct accgatgata
     4021 ttaagttgga ttataaatac taacgaaaag aaagcacaac ttgagcttta taatgaagtg
     4081 gctcaagaat atgggcacga tattcataat atcgaccatt gcttatcata tataacatct
     4141 gtagatcatg actcaattaa agcgaaagag atttgccgga aatttctggg gcattggtat
     4201 gattcttatg tgaatgctac gactattttt gatgattcag accaaacaag aggttatgat
     4261 ttcaataaag ggcagtggcg tgactttgta ttaaaaggac ataaagatac taatcgccgt
     4321 attgattaca gttacgaaat caatcccgtg ggaacgccgc aggaatgtat tgacataatt
     4381 caaaaagaca ttgatgctac aggaatatca aatatttgtt gtggatttga agctaatgga
     4441 acagtagacg aaattattgc ttccatgaag ctcttccagt ctgatgtcat gccatttctt
     4501 aaagaaaaac aacgttcgct attatattag ctaaggagaa agaaatgaaa tttggattgt
     4561 tcttccttaa cttcatcaat tcaacaactg ttcaagaaca aagtatagtt cgcatgcagg
     4621 aaataacgga gtatgttgat aagttgaatt ttgaacagat tttagtgtat gaaaatcatt
     4681 tttcagataa tggtgttgtc ggcgctcctc tgactgtttc tggttttctg ctcggtttaa
     4741 cagagaaaat taaaattggt tcattaaatc acatcattac aactcatcat cctgtccgca
     4801 tagcggagga agcttgctta ttggatcagt taagtgaagg gagatttatt ttagggttta
     4861 gtgattgcga aaaaaaagat gaaatgcatt tttttaatcg cccggttgaa tatcaacagc
     4921 aactatttga agagtgttat gaaatcatta acgatgcttt aacaacaggc tattgtaatc
     4981 cagataacga tttttatagc ttccctaaaa tatctgtaaa tccccatgct tatacgccag
     5041 gcggacctcg gaaatatgta acagcaacca gtcatcatat tgttgagtgg gcggccaaaa
     5101 aaggtattcc tctcatcttt aagtgggatg attctaatga tgttagatat gaatatgctg
     5161 aaagatataa agccgttgcg gataaatatg acgttgacct atcagagata gaccatcagt
     5221 taatgatatt agttaactat aacgaagata gtaataaagc taaacaagag acgcgtgcat
     5281 ttattagtga ttatgttctt gaaatgcacc ctaatgaaaa tttcgaaaat aaacttgaag
     5341 aaataattgc agaaaacgct gtcggaaatt atacggagtg tataactgcg gctaagttgg
     5401 caattgaaaa gtgtggtgcg aaaagtgtat tgctgtcctt tgaaccaatg aatgatttga
     5461 tgagccaaaa aaatgtaatc aatattgttg atgataatat taagaagtac cacatggaat
     5521 atacctaata gatttcgagt tgcagcgagg cggcaagtga acgaatcccc aggagcatag
     5581 ataactatgt gactggggtg agtgaaagca gccaacaaag cagcagcttg aaagatgaag
     5641 ggtataaaag agtatgacag cagtgctgcc atactttcta atattatctt gaggagtaaa
     5701 acaggtatga cttcatatgt tgataaacaa gaaattacag caagctcaga aattgatgat
     5761 ttgatttttt cgagcgatcc attagtgtgg tcttacgacg agcaggaaaa aatcagaaag
     5821 aaacttgtgc ttgatgcatt tcgtaatcat tataaacatt gtcgagaata tcgtcactac
     5881 tgtcaggcac acaaagtaga tgacaatatt acggaaattg atgacatacc tgtattccca
     5941 acatcggttt ttaagtttac tcgcttatta acttctcagg aaaacgagat tgaaagttgg
     6001 tttaccagta gcggcacgaa tggtttaaaa agtcaggtgg cgcgtgacag attaagtatt
     6061 gagagactct taggctctgt gagttatggc atgaaatatg ttggtagttg gtttgatcat
     6121 caaatagaat tagtcaattt gggaccagat agatttaatg ctcataatat ttggtttaaa
     6181 tatgttatga gtttggtgga attgttatat cctacgacat ttaccgtaac agaagaacga
     6241 atagattttg ttaaaacatt gaatagtctt gaacgaataa aaaatcaagg gaaagatctt
     6301 tgtcttattg gttcgccata ctttatttat ttactctgcc attatatgaa agataaaaaa
     6361 atctcatttt ctggagataa aagcctttat atcataaccg gaggcggctg gaaaagttac
     6421 gaaaaagaat ctctgaaacg tgatgatttc aatcatcttt tatttgatac tttcaatctc
     6481 agtgatatta gtcagatccg agatatattt aatcaagttg aactcaacac ttgtttcttt
     6541 gaggatgaaa tgcagcgtaa acatgttccg ccgtgggtat atgcgcgagc gcttgatcct
     6601 gaaacgttga aacctgtacc tgatggaacg ccggggttga tgagttatat ggatgcgtca
     6661 gcaaccagtt atccagcatt tattgttacc gatgatgtcg ggataattag cagagaatat
     6721 ggtaagtatc ccggcgtgct cgttgaaatt ttacgtcgcg tcaatacgag gacgcagaaa
     6781 gggtgtgctt taagcttaac cgaagcgttt gatagttgat gacctgcagg catgcaagct
     6841 tgcggccgcg ggccccatgg atcgatagct ggtcgacaca atctgccctt tcgaaagatc
     6901 ccaacgaaaa gcgtgaccac atggtccttc ttgagtttgt aactgctgct gggattacac
     6961 atggcatgga tgagctctac aaataatgag ctagcccgcc taatgagcgg gctttttttt
     7021 ctcggcctag gagatactta acagggaagt gagagggccg cggcaaagcc gttcttccgc
     7081 ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg tgtcagctca
     7141 ctcaaaagcg gtaatacggt tatccacaga atcaggggat aaagccggaa agaacatgtg
     7201 agcaaaaagc aaagcaccgg aagaagccaa cgccgcaggc gtttttccat aggctccgcc
     7261 cccctgacga gcatcacaaa aatcgacgct caagccagag gtggcgaaac ccgacaggac
     7321 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7381 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     7441 gctcacgctg ttggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7501 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7561 acccggtaag acacgactta tcgccactgg cagcagccat tggtaactga tttagaggac
     7621 tttgtcttga agttatgcac ctgttaaggc taaactgaaa gaacagattt tggtgagtgc
     7681 ggtcctccaa cccacttacc ttggttcaaa gagttggtag ctcagcgaac cttgagaaaa
     7741 ccaccgttgg tagcggtggt ttttctttat ttatgagatg atgaatcaat cggtctatca
     7801 agtcaacgaa cagctattcc gttgaagatc atcttattaa tcagataaaa tatttctagg
     7861 atctaaaaca ctaggccccg tcaggatggc cttctgctta atttgatgcc tggcagttta
     7921 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc gcaacgttca aatccgctcc
     7981 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
     8041 ccagtctttc gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcat
     8101 ggggagaccc cacactacca tcggcgctac ggcgtttcac ttctgagttc ggcatggggt
     8161 caggtgggac caccgcgcta ctgccgccag gcaaattctg ttttatcaga ccgcttctgc
     8221 gttctgattt aatctgtatc aggctgaaaa tcttctctca tccgccaaaa cagccaagct
     8281 ggatctggca aatcgctgaa tattcctttt gtctccgacc atcaggcacc tgagtcgctg
     8341 tctttttcgt gacattcagt tcgctgcgct cacggctctg gcagtgaatg ggggtaaatg
     8401 gcactacagg cgccttttat ggattcatgc aaggaaacta cccataatac aagaaaagcc
     8461 cgtcacgggc ttctcagggc gttttatggc gggtctgcta tgtggtgcta tctgactttt
     8521 tgctgttcag cagttcctgc cctctgattt tccagtctga ccacttcgga ttatcccgtg
     8581 acaggtcatt cagactggct aatgcaccca gtaaggcagc ggtatcatca acaggcttac
     8641 ccgtcttact gtcaaccgga tctaaaacac tagttaaggg attttggtca tgagattatc
     8701 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     8761 tatatatgag taaacttggt ctgacagtta cgccccgccc tgccactcat cgcagtactg
     8821 ttgtaattca ttaagcattc tgccgacatg gaagccatca cagacggcat gatgaacctg
     8881 aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tagtgaaaac
     8941 gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca
     9001 gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt
     9061 ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg
     9121 gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg
     9181 gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgga actccggatg
     9241 agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt
     9301 ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg
     9361 agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt
     9421 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa
     9481 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac
     9541 gtgccgatca acgtctcatt ttcgccaaaa gttggcccag ggcttcccgg tatcaacagg
     9601 gacaccagga tttatttatt ctgcgaagtg atcttccgcc ctttcgtctt ctagacaaca
     9661 tcagcaagga gaaaggggct accggcgaac cagcagcccc tttataaagg cgcttcagta
     9721 gtcagaccag catcagtcct gaaaaggcgg gcctgcgccc gcctccaggt tgctacttac
     9781 cggattcgta agccatgaaa gccgccacct ccctgtgtcc gtctctgtaa cgaatctcgc
     9841 acagcgattt tcgtgtcaga taagtgaata tcaacagtgt gagacacacg atcaacacac
     9901 accagacaag ggaacttcgt ggtagtttca tggccttctt ctccttgcgc aaagcgcggt
     9961 aagaggctat cctgatgtgg actagacata gggatgcctc gtggtggtta atgaaaatta
    10021 acttactacg gggctatctt ctttctgcca cacaacacgg caacaaacca ccttcacgtc
    10081 atgaggcaga aagcctcaag cgccgggcac atcatagccc atatacctgc acgctgacca
    10141 cactcacttt ccctgaaaat aatccgctca ttcagaccgt tcacgggaaa tccgtgtgat
    10201 tgttgccgca tcacgctgcc tcccggagtt tgtctcgagc acttttgtta cccgccaaac
    10261 aaaacccaaa aacaactcat acccaaccca ataaaacacc aaaacaagac aaataatcat
    10321 tgattgatgg ttgaaatggg gtaaacttga caaacaaacc cacttaaaac ccaaaacata
    10381 cccaaacaca caccaaaaaa acaccataag gagttttata aatgttggta ttcattgatg
    10441 acggttcaac aaacatcaaa ctacagtagc aggaaagcga cggaacaatt aaacagcaca
    10501 ttagcccgaa cagcttcaaa cgcgagtggg cagtctcttt tggtgataaa aaggtcttta
    10561 actacacact gaacggcgaa cagtattcat ttgatccaat cagcccggat gctgtagtca
    10621 caaccaatat cgcatggcaa tacagcgacg ttaatgtcgt tgcagtgcat cacgccttac
    10681 tgaccagtgg tctgccggta agcgaagtgg atattgtttg cacacttcct ctgacagagt
    10741 attacgacag aaataaccaa cccaatacgg aaaatattga gcgtaagaaa gcaaacttcc
    10801 ggaaaaaaat tacattaaat ggcggggata cattcacaat aaaagatgta aaagtcatgc
    10861 ctgaatctat accggcaggt tatgaagttc tacaagaact ggatgagtta gattctttat
    10921 taattataga tctcgggggc accacattag atatttctca ggtaatgggg aaattatcgg
    10981 ggatcagtaa aatatacgga gactcatctc ttggtgtctc tctggttaca tctgcagtaa
    11041 aagatgccct ttctcttgcg agaacaaaag gaagtagcta tcttgctgac gatataatca
    11101 ttcacagaaa aga
//
