LOCUS       V013914                 5274 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013914
VERSION     V013914
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5274
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     5'UTR           41..83
                     /label="Xenopus globin 5'-UTR"
                     /note="translational enhancer from the 5'-UTR of the major
                     beta-globin gene of Xenopus laevis"
     CDS             154..861
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     CDS             898..2322
                     /gene="TRIM21"
                     /label="E3 ubiquitin-protein ligase TRIM21"
                     /note="E3 ubiquitin-protein ligase TRIM21 from Homo
                     sapiens. Accession#: P19474"
     3'UTR           2369..2494
                     /label="Xenopus globin 3'-UTR"
                     /note="3'-UTR of the major beta-globin gene of Xenopus
                     laevis"
     promoter        complement(2599..2617)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2635..2651)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2659..2675)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2683..2713)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2728..2749)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3037..3625)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3799..4656)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4657..4761)
                     /label="AmpR promoter"
     primer_bind     5235..5251
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5258..5274
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg cgaattaatt cgagctcggt acccagcttg cttgttcttt ttgcagaagc
       61 tcagaataaa cgctcaactt tggcagaatg aagcggcccg ctgctactaa gaaggctggt
      121 caggctaaga agaagaagct accggtcgcc accatggtga gcaagggcga ggaggataac
      181 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
      241 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
      301 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
      361 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
      421 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
      481 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
      541 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
      601 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
      661 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
      721 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
      781 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
      841 ggcatggacg agctgtacaa gtccggactc agatctcgag ctcaagcttc gaattcaatg
      901 gcttcagcag cacgcttgac aatgatgtgg gaggaggtca catgccctat ctgcctggac
      961 cccttcgtgg agcctgtgag catcgagtgt ggccacagct tctgccagga atgcatctct
     1021 caggttggga aaggtggggg cagcgtctgt cctgtgtgcc ggcagcgctt tctgctcaag
     1081 aatctccggc ccaatcgaca gctagccaac atggtgaaca accttaaaga aatcagccag
     1141 gaggccagag agggcacaca gggggaacgg tgtgcagtgc atggagagag acttcacctg
     1201 ttctgtgaga aagatgggaa ggccctttgc tgggtatgtg cccagtctcg gaaacaccgt
     1261 gaccacgcca tggtccctct tgaggaggct gcacaggagt accaggagaa gctccaggtg
     1321 gcattagggg aactgagaag aaagcaggag ttggctgaga agttggaagt ggaaattgca
     1381 ataaagagag cagactggaa gaaaacagtg gaaacacaga aatctaggat tcacgcagag
     1441 tttgtgcagc aaaaaaactt cctggttgaa gaagaacaga ggcagctgca ggagctggag
     1501 aaggatgaga gggagcagct gagaatcctg ggggagaaag aggccaagct ggcccagcag
     1561 agccaggccc tacaggagct catctcagag ctagatcgaa ggtgccacag ctcagcactg
     1621 gaactgctgc aggaggtgat aattgtcctg gaaaggagtg agtcctggaa cctgaaggac
     1681 ctggatatta cctctccaga actcaggagt gtgtgccatg tgccagggct gaagaagatg
     1741 ctgaggacat gtgcagtcca catcactctg gatccagaca cagccaatcc gtggctgata
     1801 ctttcagaag atcggagaca agtgaggctt ggagacaccc agcagagcat acctggaaat
     1861 gaagagagat ttgatagtta tcctatggtc ctgggtgccc agcactttca ctctggaaaa
     1921 cattactggg aggtagatgt gacaggaaag gaggcctggg acctgggtgt ctgcagagac
     1981 tctgtgcgca ggaaggggca ctttttgctt agttccaaga gtggcttctg gacaatttgg
     2041 ttgtggaaca aacaaaaata tgaggctggc acctaccccc agactcccct ccaccttcag
     2101 gtgcctccat gccaagttgg gattttcctg gactatgagg ctggcatggt ctccttctac
     2161 aacatcactg accatggctc cctcatctac tccttctctg aatgtgcctt tacaggacct
     2221 ctgcggccct tcttcagtcc tggtttcaat gatggaggaa aaaacacagc ccctctaacc
     2281 ctctgtccac tgaatattgg atcacaagga tccactgact attgagcggc cgctctagat
     2341 aaatgatagc ttgatctggt taccactaaa ccagcctcaa gaacacccga atggagtctc
     2401 taagctacat aataccaact tacactttac aaaatgttgt cccccaaaat gtagccattc
     2461 gtatctgctc ctaataaaaa gaaagtttct tcacattcta aaaaaaaaaa aaaaaaaaaa
     2521 aaaaaaaaaa aaaccccccc cccccccccc tgcaggcgcg ccgccggcga tttaaattaa
     2581 ttaatctagc ttgagtattc tatagtgtca cctaaatagc ttggcgtaat catggtcata
     2641 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     2701 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     2761 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     2821 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     2881 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     2941 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     3001 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     3061 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     3121 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     3181 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     3241 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     3301 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     3361 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     3421 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     3481 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     3541 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     3601 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     3661 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     3721 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     3781 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     3841 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     3901 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     3961 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     4021 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     4081 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     4141 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     4201 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     4261 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     4321 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     4381 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     4441 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     4501 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     4561 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     4621 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     4681 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     4741 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     4801 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     4861 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     4921 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     4981 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     5041 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
     5101 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     5161 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     5221 ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tata
//
