LOCUS       40924_21379        3257 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGEM58ZNf(-), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Hwang S-K.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-OCT-2000) Div. of Biochemstry, NIAST, RDA, 249 
            Seodun-dong, Gwonsun-gu, Suwon 441-707, Korea
REFERENCE   2  (bases 1 to 3257)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3257)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (02-OCT-2000) Div. of Biochemstry, NIAST, RDA, 249 Seodun-dong, 
            Gwonsun-gu, Suwon 441-707, Korea"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3257
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    5..121
                     /note="multicloning site; EcoRI, SacI, KpnI, NruI, NsiI,
                     XbaI, EcoRV, BamHI, AvaI, SmaI, ApaI, SalI (AccI, HincII), 
                     PstI, NotI, SpeI, EcoRV, SacII, NcoI, SphI, HindIII'; 
                     EcoRI-HindIII fragment of pUC58 (AF253496) was ligated with
                     the EcoRI-HindIII gap of pGEM-3Zf(-); insertion did not 
                     affect a blue/white selection"
     regulatory      95..104
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(128..146)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(164..180)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(188..204)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(212..242)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(257..278)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(566..1154)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1328..2185)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2186..2290)
                     /label=AmpR promoter
     rep_origin      2622..3077
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3218..3234
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3241..3257
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattc gagctcggta cctcgcgaat gcatctagat atcggatccc gggcccgtcg
       61 acctgcaggc ggccgcacta gtgatatccc gcggccatgg cggccgggag catgcaagct
      121 tgagtattct atagtgtcac ctaaatagct tggcgtaatc atggtcatag ctgtttcctg
      181 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
      241 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
      301 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
      361 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
      421 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
      481 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
      541 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      601 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      661 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      721 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      781 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      841 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      901 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      961 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     1021 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     1081 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     1141 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     1201 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     1261 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     1321 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     1381 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1441 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     1501 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1561 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1621 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1681 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1741 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     1801 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     1861 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     1921 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     1981 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     2041 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     2101 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     2161 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     2221 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     2281 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     2341 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     2401 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     2461 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     2521 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     2581 aaataccgca cagatgcgta aggagaaaat accgcatcag gacgcgccct gtagcggcgc
     2641 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     2701 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     2761 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     2821 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     2881 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     2941 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     3001 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     3061 attaacgctt acaatttcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     3121 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     3181 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattg
     3241 taatacgact cactata
//
