LOCUS       40924_21374        4464 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGEM4Z-U1-EGFP-RhoAtest1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Koch JC, Barski E, Lingor P, Bahr M, Michel U.
  TITLE     Plasmids containing NRSE/RE1 sites enhance neurite outgrowth of 
            retinal ganglion cells via sequestration of REST independent of NRSE
            dsRNA expression
  JOURNAL   FEBS J. 278 (18), 3472-3483 (2011)
  PUBMED    21790997
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Koch JC, Michel U.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2010) Neurology, University Medicine Goettingen, 
            Robert-Koch-Str. 40, Goettingen, Niedersachsen 37075, Germany
REFERENCE   3  (bases 1 to 4464)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4464)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS J."; 
            date: "2011"; volume: "278"; issue: "18"; pages: "3472-3483"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-OCT-2010) Neurology, University Medicine Goettingen, 
            Robert-Koch-Str. 40, Goettingen, Niedersachsen 37075, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(46..180)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     intron          complement(196..329)
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     misc_feature    354..524
                     /label=partial sequence of RhoA RNA
                     /note="partial sequence of RhoA RNA"
     CDS             complement(549..1265)
                     /label=EGFP
                     /note="enhanced GFP"
     primer_bind     complement(1318..1334)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      1354..1745
                     /label=U1 snRNA promoter
                     /note="U1 snRNA promoter"
                     /regulatory_class="promoter"
     promoter        complement(1787..1805)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1824..1840)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1848..1864)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1872..1902)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1917..1938)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2226..2814)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2988..3845)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3846..3950)
                     /label=AmpR promoter
     primer_bind     4424..4440
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4448..4464
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaatacgaat tcgagctcgg tacccgggga tcttcgatgc tagacgatcc agacatgata
       61 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
      121 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
      181 gctcgaagtc gacacctgtg gagagaaagg caaagtggat gtcagtaaga ccaataggtg
      241 cctatcagaa acgcaagagt cttctctgtc tcgacaagcc cagtttctat gtggtctcct
      301 taaacctgtc ttgtaacctt gatacttacc tgcggccgca ctagtataga tctccagctg
      361 tgtcccataa agccaactct acctgcttcc cgtccacttc aatatctgcc acatagtttt
      421 caaacaccgt gggcacataa acctctggga actggtcctt gctgaagact atgagcaagc
      481 atgtcttacc acaagctcca tcaccaacaa tcaccgggcc ctatcaattg acgcgtgcgg
      541 ccgctttact tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc
      601 agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga ctgggtgctc
      661 aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag
      721 tggtcggcga gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg
      781 atgccgttct tctgcttgtc ggccatgata tagacgttgt ggctgttgta gttgtactcc
      841 agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg
      901 ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc
      961 ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg
     1021 tgctgcttca tgtggtcggg gtagcggctg aagcactgca cgccgtaggt cagggtggtc
     1081 acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc
     1141 ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg
     1201 tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc
     1261 accatggtgg cgaccggtgg atccttgcta gcagcttgaa ttcgatatca agcttatcga
     1321 taccgtcgac ctcgaggggg ggcccggtac cgatgatctt gggcctctgc cccgacacag
     1381 cctcatacgc ctcactcttt acacacacgg tcacttgccc cgcgcactcc cgagcccttt
     1441 ccagccctga cacacagctg ggattctcac ttccgatcag cggtcctgaa cccgctccca
     1501 gggcacggga actccttcgt ggtgaagcag caagtggcga agacgtcagc ctctgcgctg
     1561 cctcatctac atagaagtcg ccctgtccgt gatgtcaccg acagtgcctt gcccagtccc
     1621 cgtctgcctt tctgccactc aaccgaccaa tctgctgcca gagccgccaa ggggaagtga
     1681 cgtctgcctc tccctttttc cctcccgccc ctgcgtctgt tctctcccaa agaagctggt
     1741 ccttaaaaaa atctagagtc gacctgcagg catgcaagct tgtctcccta tagtgagtcg
     1801 tattagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     1861 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     1921 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttcgagtc gggaaacctg
     1981 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     2041 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     2101 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     2161 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     2221 gcgtttttcg ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     2281 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     2341 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     2401 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     2461 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     2521 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     2581 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     2641 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     2701 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     2761 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     2821 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     2881 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     2941 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     3001 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     3061 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     3121 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     3181 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     3241 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tggcattgct
     3301 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     3361 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     3421 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     3481 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     3541 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     3601 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     3661 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     3721 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     3781 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     3841 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     3901 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     3961 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     4021 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
     4081 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     4141 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
     4201 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     4261 aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg
     4321 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
     4381 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
     4441 gaattggatt taggtgacac tata
//
