LOCUS       pGEM-luc.        4931 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Plasmid carrying the firefly luciferase gene flanked by unique 
            restriction sites.
ACCESSION   .
VERSION     .
KEYWORDS    pGEM-luc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4931)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Derived from pGEM(R)-11Zf(–).
FEATURES             Location/Qualifiers
     source          1..4931
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    9..50
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             complement(105..1754)
                     /label=luciferase
                     /note="firefly luciferase"
     misc_feature    1757..1795
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(1802..1820)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1838..1854)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1862..1878)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1886..1916)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1931..1952)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2240..2828)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3002..3859)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3860..3964)
                     /label=AmpR promoter
     rep_origin      4296..4751
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     4892..4908
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4915..4931
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg gccaagtcgg ccgagctcga attcgtcgac ctcgaggcct cggaggatta
       61 caatagctaa gaatttcgtc atcgctgaat acagttacat tttacaattt ggactttccg
      121 cccttcttgg cctttatgag gatctctctg atttttcttg cgtcgagttt tccggtaaga
      181 cctttcggta cttcgtccac aaacacaact cctccgcgca actttttcgc ggttgttact
      241 tgactggcga cgtaatccac gatctctttt tccgtcatcg tctttccgtg ctccaaaaca
      301 acaacggcgg cgggaagttc accggcgtca tcgtcgggaa gacctgccac gcccgcgtcg
      361 aagatgttgg ggtgttgtaa caatatcgat tccaattcag cgggggccac ctgatatcct
      421 ttgtatttaa ttaaagactt caagcggtca actatgaaga agtgttcgtc ttcgtcccag
      481 taagctatgt ctccagaatg tagccatcca tccttgtcaa tcaaggcgtt ggtcgcttcc
      541 ggattgttta cataaccgga cataatcata ggtcctctga cacataattc gcctctctga
      601 ttaacgccca gcgttttccc ggtatccaga tccacaacct tcgcttcaaa aaatggaaca
      661 actttaccga ccgcgcccgg tttatcatcc ccctcgggtg taatcagaat agctgatgta
      721 gtctcagtga gcccatatcc ttgtcgtatc cctggaagat ggaagcgttt tgcaaccgct
      781 tccccgactt ctttcgaaag aggtgcgccc ccagaagcaa tttcgtgtaa attagataaa
      841 tcgtatttgt caatcagagt gcttttggcg aagaatgaaa atagggttgg tactagcaac
      901 gcactttgaa ttttgtaatc ctgaagggat cgtaaaaaca gctcttcttc aaatctatac
      961 attaagacga ctcgaaatcc acatatcaaa tatccgagtg tagtaaacat tccaaaaccg
     1021 tgatggaatg gaacaacact taaaatcgca gtatccggaa tgatttgatt gccaaaaata
     1081 ggatctctgg catgcgagaa tctgacgcag gcagttctat gcggaagggc cacaccctta
     1141 ggtaacccag tagatccaga ggaattcatt atcagtgcaa ttgttttgtc acgatcaaag
     1201 gactctggta caaaatcgta ttcattaaaa ccgggaggta gatgagatgt gacgaacgtg
     1261 tacatcgact gaaatccctg gtaatccgtt ttagaatcca tgataataat tttctggatt
     1321 attggtaatt ttttttgcac gttcaaaatt ttttgcaacc cctttttgga aacaaacact
     1381 acggtaggct gcgaaatgtt catactgttg agcaattcac gttcattata aatgtcgttc
     1441 gcgggcgcaa ctgcaactcc gataaataac gcgcccaaca ccggcataaa gaattgaaga
     1501 gagttttcac tgcatacgac gattctgtga tttgtattca gcccatatcg tttcatagct
     1561 tctgccaacc gaacggacat ttcgaagtat tccgcgtacg tgatgttcac ctcgatatgt
     1621 gcatctgtaa aagcaattgt tccaggaacc agggcgtatc tcttcatagc cttatgcagt
     1681 tgctctccag cggttccatc ctctagagga tagaatggcg ccgggccttt ctttatgttt
     1741 ttggcgtctt ccatttggat ccgggccctc tagatgcggc cgcatgcata agcttgagta
     1801 ttctatagtg tcacctaaat agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     1861 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     1921 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     1981 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     2041 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     2101 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     2161 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     2221 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     2281 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     2341 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     2401 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     2461 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     2521 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     2581 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     2641 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     2701 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     2761 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     2821 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     2881 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     2941 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     3001 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     3061 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     3121 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     3181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     3241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     3301 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     3361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     3421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     3481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     3541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     3601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     3661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     3721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     3781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     3841 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     3901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     3961 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     4021 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
     4081 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     4141 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
     4201 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     4261 cgcacagatg cgtaaggaga aaataccgca tcaggacgcg ccctgtagcg gcgcattaag
     4321 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     4381 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     4441 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccgcaa
     4501 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     4561 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     4621 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     4681 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     4741 gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     4801 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     4861 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac
     4921 gactcactat a
//
