LOCUS       40924_21369        3130 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGEM-WIZ, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Bao S, Cagan R.
  TITLE     Fast cloning inverted repeats for RNA interference
  JOURNAL   RNA 12 (11), 2020-2024 (2006)
  PUBMED    17005926
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Bao S, Cagan R.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2006) Molecular Biology and Pharmacology, 
            Washington University, 660 South Euclid Ave, St. Louis, MO 63110, 
            USA
REFERENCE   3  (bases 1 to 3130)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3130)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA"; date:
            "2006"; volume: "12"; issue: "11"; pages: "2020-2024"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-AUG-2006) Molecular Biology and Pharmacology, Washington 
            University, 660 South Euclid Ave, St. Louis, MO 63110, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    10..243
                     /label=multiple cloning region
                     /note="multiple cloning region"
     regulatory      33..42
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          77..150
                     /number=2
                     /note="white gene"
     promoter        complement(255..273)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(291..307)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(315..331)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(339..369)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(384..405)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(693..1281)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1455..2312)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2313..2417)
                     /label=AmpR promoter
     rep_origin      complement(2495..2950)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3091..3107
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3114..3130
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggccgcggga ttagatctct
       61 cgagactagt gcctaggtga gtttctattc gcagtcggct gatctgtgtg aaatcttaat
      121 gaagggtcca attaccaatt tgaaactcag ctagctacgc gttctagaga attcaagctt
      181 aatcactagt gcggccgcct gcaggtcgac catatgggag agctcccaac gcgttggatg
      241 catagcttga gtattctata gtgtcaccta aatagcttgg cgtaatcatg gtcatagctg
      301 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
      361 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
      421 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
      481 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
      541 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
      601 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
      661 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
      721 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
      781 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
      841 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
      901 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
      961 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     1021 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     1081 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     1141 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     1201 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1261 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     1321 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     1381 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     1441 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     1501 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     1561 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     1621 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     1681 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     1741 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     1801 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     1861 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     1921 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     1981 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     2041 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     2101 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     2161 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     2221 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     2281 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     2341 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     2401 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgatgcggt gtgaaatacc
     2461 gcacagatgc gtaaggagaa aataccgcat caggaaattg taagcgttaa tattttgtta
     2521 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     2581 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     2641 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     2701 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     2761 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     2821 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     2881 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     2941 cagggcgcgt ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     3001 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     3061 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg
     3121 actcactata
//
