LOCUS       V006064                 4350 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006064
VERSION     V006064
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Wilson RB, Davis D, Mitchell AP.
  TITLE     Rapid hypothesis testing with Candida albicans through gene
            disruption with short homology regions
  JOURNAL   J. Bacteriol. 181 (6), 1868-1874 (1999)
   PUBMED   10074081
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Wilson RB, Davis D, Mitchell AP.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-AUG-1999) Microbiology, Columbia University, 701 W.
            168th St., New York, NY 10032, USA
REFERENCE   3  (bases 1 to 4350)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4350)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Bacteriol."; date: "1999"; volume: "181"; issue: "6"; pages:
            "1868-1874"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-AUG-1999) Microbiology, Columbia University, 701 W. 168th St.,
            New York, NY 10032, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(75..93)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(111..127)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(135..151)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(159..189)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(204..225)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(513..1101)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1275..2132)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2133..2237)
                     /label="AmpR promoter"
     rep_origin      complement(2318..2773)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     2914..2930
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2937..2955
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2986..2995
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3409..4218
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
ORIGIN
        1 aatcactagt gcggccgcct gcaggtcgac catatgggag agctcccaac gcgttggatg
       61 catagcttga gtattctata gtgtcaccta aatagcttgg cgtaatcatg gtcatagctg
      121 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
      181 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
      241 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
      301 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
      361 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
      421 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
      481 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
      541 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
      601 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
      661 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
      721 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
      781 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
      841 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
      901 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
      961 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     1021 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1081 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     1141 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     1201 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     1261 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     1321 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     1381 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     1441 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     1501 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     1561 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     1621 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     1681 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     1741 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     1801 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     1861 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     1921 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     1981 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     2041 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     2101 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     2161 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     2221 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgtatgcgg tgtgaaatac
     2281 cgcacagatg cgtaaggaga aaataccgca tcaggcgaaa ttgtaaacgt taatattttg
     2341 ttaaaattcg cgttaaatat ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     2401 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     2461 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     2521 tatcagggcg atggcccact acgtgaacca tcacccaaat caagtttttt gcggtcgagg
     2581 tgccgtaaag ctctaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     2641 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     2701 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     2761 ctacagggcg cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     2821 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     2881 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat
     2941 acgactcact atagggcgaa ttgggcccga cgtcgcatgc tcccggccgc catggccgcg
     3001 ggatttggat ggtataaacg gaaacaaaaa aaagagctgg tactactttc tttaaaatta
     3061 ttttattatt tgattttatt taatagtata tattatattt tgaacgtaga ttattttgtt
     3121 gaaagttgct gtagtgccat tgattcgtaa cactaattct gtattagtca ttcctcttgt
     3181 ttgatagtat ccaaaaaaac ggctattttt ttgcaatctt atttcctgca tattatacag
     3241 ataacataat gaaagaaaaa atcttttttt ttgttcttca atgatgattt caaccattct
     3301 tttaaacatt gatcaattcc tgagcaacaa ccccatacac actggtttat ataccgcccc
     3361 ttttacagtt gaagaaagaa atagaaatag aaatagcaaa caaaagatat gacagtcaac
     3421 actaagacct atagtgagag agcagaaact catgcctcac cagtagcaca gcgattattt
     3481 cgattaatgg aactgaagaa aaccaattta tgtgcatcaa ttgacgttga taccactaag
     3541 gaattccttg aattaattga taaattaggt ccttatgtat gcttaatcaa gactcatatt
     3601 gatataatca atgatttttc ctatgaatcc actattgaac cattattaga actttcacgt
     3661 aaacatcaat ttatgatttt tgaagataga aaatttgctg atattggtaa taccgtaaag
     3721 aaacaatata ttggtggagt ttataaaatt agtagttggg cagatattac caatgctcat
     3781 ggtgtcactg ggaatggagt ggttgaagga ttaaaacagg gagctaaaga aaccaccacc
     3841 aaccaagagc caagagggtt attgatgtta gctgaattat catcagtggg atcattagca
     3901 tatggagaat attctcaaaa aactgttgaa attgctaaat ccgataagga atttgttatt
     3961 ggatttattg cccaacgtga tatgggtggc caagaagaag gatttgattg gcttattatg
     4021 acacctggag ttggattaga tgataaaggt gatggattag gacaacaata tagaactgtt
     4081 gatgaagttg ttagcactgg aactgatatt atcattgttg gtagaggatt gtttggtaaa
     4141 ggaagagatc cagatattga aggtaaaagg tatagaaatg ctggttggaa tgcttatttg
     4201 aaaaagactg gccaattata aatgtgaagg gggagatttt cactttatta gatttgtata
     4261 tatgtagaat aaataaataa ataagttaaa taaataatta aataagggtg gtaattatta
     4321 ctatttacaa tcaaaggtgg tccttctaga
//
