LOCUS       40924_21344        3038 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGEM-S1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Bao S, Cagan R.
  TITLE     Fast cloning inverted repeats for RNA interference
  JOURNAL   RNA 12 (11), 2020-2024 (2006)
  PUBMED    17005926
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Bao S, Cagan R.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2006) Molecular Biology and Pharmacology, 
            Washington University, 660 South Euclid Ave, St. Louis, MO 63110, 
            USA
REFERENCE   3  (bases 1 to 3038)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3038)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA"; date:
            "2006"; volume: "12"; issue: "11"; pages: "2020-2024"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-AUG-2006) Molecular Biology and Pharmacology, Washington 
            University, 660 South Euclid Ave, St. Louis, MO 63110, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    10..151
                     /label=multiple cloning region
                     /note="multiple cloning region"
     regulatory      33..42
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(163..181)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(199..215)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(223..239)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(247..277)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(292..313)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(601..1189)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1363..2220)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2221..2325)
                     /label=AmpR promoter
     rep_origin      complement(2403..2858)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     2999..3015
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3022..3038
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggccgcggga ttagatctct
       61 cgagggtacc tctagagaat tcaagcttaa tcactagtgc ggccgcctgc aggtcgacca
      121 tatgggagag ctcccaacgc gttggatgca tagcttgagt attctatagt gtcacctaaa
      181 tagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
      241 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
      301 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
      361 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
      421 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
      481 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
      541 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
      601 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      661 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      721 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      781 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      841 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      901 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      961 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1021 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     1081 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1141 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1201 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1261 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     1321 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     1381 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     1441 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     1501 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     1561 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1621 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1681 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1741 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1801 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1861 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1921 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1981 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     2041 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     2101 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     2161 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     2221 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     2281 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     2341 agtgccacct gatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     2401 ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     2461 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     2521 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     2581 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     2641 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     2701 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     2761 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     2821 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt caggctgcgc
     2881 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     2941 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     3001 aaaacgacgg ccagtgaatt gtaatacgac tcactata
//
