LOCUS       V006067                 5558 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006067
VERSION     V006067
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Wilson RB, Davis D, Mitchell AP.
  TITLE     Rapid hypothesis testing with Candida albicans through gene
            disruption with short homology regions
  JOURNAL   J. Bacteriol. 181 (6), 1868-1874 (1999)
   PUBMED   10074081
REFERENCE   2  (bases 1 to 5558)
  AUTHORS   Wilson RB, Davis D, Mitchell AP.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-AUG-1999) Microbiology, Columbia University, 701 W.
            168th St., New York, NY 10032, USA
REFERENCE   3  (bases 1 to 5558)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5558)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Bacteriol."; date: "1999"; volume: "181"; issue: "6"; pages:
            "1868-1874"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-AUG-1999) Microbiology, Columbia University, 701 W. 168th St.,
            New York, NY 10032, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5558
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(50..68)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(86..102)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(110..126)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(134..164)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(179..200)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(488..1076)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1250..2107)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2108..2212)
                     /label="AmpR promoter"
     rep_origin      complement(2293..2748)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     2889..2905
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2912..2930
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2961..2970
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3514..4407
                     /gene="HIS1"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Candida albicans
                     (strain SC5314 / ATCC MYA-2876). Accession#: P46586"
ORIGIN
        1 tcgaccatat gggagagctc ccaacgcgtt ggatgcatag cttgagtatt ctatagtgtc
       61 acctaaatag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
      121 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
      181 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
      241 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
      301 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
      361 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
      421 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      481 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      541 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
      601 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
      661 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
      721 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
      781 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
      841 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
      901 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
      961 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     1021 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     1081 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     1141 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     1201 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     1261 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     1321 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     1381 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     1441 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     1501 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     1561 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     1621 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     1681 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     1741 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     1801 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     1861 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     1921 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     1981 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     2041 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     2101 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     2161 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     2221 cccgaaaagt gccacctgta tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     2281 ccgcatcagg cgaaattgta aacgttaata ttttgttaaa attcgcgtta aatatttgtt
     2341 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     2401 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     2461 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     2521 aaccatcacc caaatcaagt tttttgcggt cgaggtgccg taaagctcta aatcggaacc
     2581 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     2641 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     2701 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt
     2761 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     2821 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     2881 acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gcgaattggg
     2941 cccgacgtcg catgctcccg gccgccatgg ccgcgggatc ctggaggatg aggagacaga
     3001 agttagtagt aacaatttgg ggacagaaga ggatacagtg acatcagagg aagaagacag
     3061 cgaaggtata gtcaaaaagc aataggacag caagacaatt tatttattta atagtttttt
     3121 ataaatgtaa aatctatcac aacatgtgta cttaattttc tgtggtaggg gaggccattt
     3181 cgttgaaacg tgtcgcgagc ggttgtgaga attgactcat tttgattatt acgcaaccat
     3241 tgaaattttt tatttttttt tggtgaagat ttttcccaca caacttcttc ttttacttaa
     3301 ccaccaacta ccgataatgg atttagtcaa tcatttacca gaccgtttgt tatttgctgt
     3361 tcctaaaaag ggcagattat acgaaaaatg ctgtaactta ttgagtggtg ccgatataca
     3421 gtttagaaga tctaatagat tagatatagc actttctaca aacttgccaa ttgcattaat
     3481 cttcttcttt tacttaacca ccaactaccg ataatggatt tagtcaatca tttaccagac
     3541 cgtttgttat ttgctgttcc taaaaagggc agattatacg aaaaatgctg taacttattg
     3601 agtggtgccg atatacagtt tagaagatct aatagattag atatagcact ttctacaaac
     3661 ttgccaattg cattaatctt cttgcctgca gctgatatcc cagttttcgt tggagaaggt
     3721 aattgtgact tgggtataac tgggttagac caaatcaaag aagctgaaca attcgacaac
     3781 atcgaggact tgttggattt gaaatttggt tcatgcaaat tgcagatcca agttccagca
     3841 gatggcgagt acgaaaagcc agaacagctt gttggaaaga aaattgtgtc ttcatttaca
     3901 aaattgagta ccgactattt caaacaattg tcagacaaac ctactaatat cagatatgtc
     3961 ggtggttccg ttgaggcttc ttgtgccttg ggtgttgctg atgctattgt cgatttggtt
     4021 gaaagtggtg aaactatgaa agcagctggg ttaaaggcca tagaaaccat attggagact
     4081 tcggctcatt tgatttcgtc aaaaaaaagt aaattcccag aaatggtcaa tataatcgtc
     4141 caaagacttc aaggtgtttt agctgcccag gaatatgtct tgtgtaacta caatgcccca
     4201 aaatccatcc aagcaaaatg tttaaccatt actccaggca gaagggccgc tactgtctct
     4261 actttggaca aacacagcga cgacgaagag gactgggttg ccatctcatc catggttaat
     4321 agaaaggaaa tcggtaatgt aatggacgaa ttgaagaaag ctggtgcaac cgatatattg
     4381 gtactcgaga tatcaaattg tagagtttaa aagaagtgat agtttctcat aaatattata
     4441 catatatact tatagatcta cttcaatttg actcaatctg tcttatacca tctttatcta
     4501 ccaccttgat gtacacacct ttaaagtcaa taggcattct cgtttgtaat tccttgacac
     4561 acatatccat taactttaaa ccatcttcga ccgtcatgtc ctttctataa tgtttatcta
     4621 acaaagatgt tgtgtaaaat gcagcgtagc catgagcacc ataagggagt tcaacttgtg
     4681 tacccaaata atctatccaa ttcaacgatg gctcattttt ctttacatca tatcctccaa
     4741 ttaaacagtt cacctggtac ggttttctag atctaaggga actggccaat tggtttctca
     4801 caaacgaggg ctgttgcctt tggagataac tcaatgtcat tttctctcat cgagtacaat
     4861 tgaatgtttg cttgaacata ctcggcaaac tgaaccgtat cacccagctt caccagtgta
     4921 tgctattaag ttgtgtgcat ttaaatgtcc ttgttttatc atctgtaccc tttaaaattg
     4981 aaatacctct tgtggcagct ttagatgttg caataagggt gccgtcagat actttaattc
     5041 ctaaaataat atccattgtg ggttggttag ttgaaataag agtgatctat aaagttctct
     5101 ttttttttgc cttcaacaaa tgtttttttt ttcatggtgg tatttttgtg cggagcgttc
     5161 tgaaaagaga atttgtacat tacttttgct atacaattca ttacttgata tattctttaa
     5221 ctttcttttc aaccaacgag ctaatcaatt tgttcaatgg gctgtcggaa ttttcaattt
     5281 gaccagttaa atttatctca acttgtttct caataaactt acccgtcaag tcaacttcaa
     5341 tgttgacagt gtcgcctact tccttcttgg ctaagataac cttttcttga ctgtagctaa
     5401 tcaacataat tgagaattga actttagatg gatcgacatt ggtaacagtc aatgaagccc
     5461 catcaatagc aataaacccc ttgtggacaa tgtaattgat aaattcttta tctcttaatt
     5521 caaaagtata ggcaatagca tttccatctg gtttcttg
//
