LOCUS       pGEM-9Zf(-).        2912 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Cloning vector with a compact MCS for in vitro RNA synthesis, ssDNA 
            production, and generation of unidirectional deletions.
ACCESSION   .
VERSION     .
KEYWORDS    pGEM-9Zf(-)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    5..53
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(54..72)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(102..118)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(126..142)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(150..180)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(195..216)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(504..1092)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1266..2123)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2124..2228)
                     /label=AmpR promoter
     rep_origin      2255..2710
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     2851..2867
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2896..2912
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaatatgcat cactagtaag ctttgctcta gactggaatt cgtcgacgag ctccctatag
       61 tgagtcgtat tagaggccga cttggccaaa ttcgtaatca tgtcatagct gtttcctgtg
      121 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
      181 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
      241 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
      301 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
      361 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      421 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      481 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
      541 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      601 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      661 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
      721 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
      781 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
      841 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
      901 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
      961 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     1021 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     1081 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     1141 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     1201 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     1261 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     1321 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     1381 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     1441 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     1501 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     1561 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     1621 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     1681 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     1741 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     1801 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     1861 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     1921 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     1981 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     2041 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     2101 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     2161 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     2221 gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc
     2281 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     2341 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     2401 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     2461 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     2521 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     2581 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     2641 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     2701 cttacaattt ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     2761 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     2821 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgactt
     2881 ggtcagcggc cgcagattta ggtgacacta ta
//
