LOCUS       40924_21305        2746 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for standard cloning and efficient in vitro RNA synthesis. 
            Identical to pGEM(R)-3Z except for the orientation of the T7 and SP6
            promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2746
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    7..63
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        complement(69..87)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(106..122)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(130..146)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(154..184)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(199..220)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(508..1096)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1270..2127)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2128..2232)
                     /label=AmpR promoter
     primer_bind     2706..2722
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2730..2746
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaatacgaat tcgagctcgg tacccgggga tcctctagag tcgacctgca ggcatgcaag
       61 cttgtctccc tatagtgagt cgtattagag cttggcgtaa tcatggtcat agctgtttcc
      121 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
      181 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
      241 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
      301 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
      361 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
      421 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
      481 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
      541 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
      601 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      661 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
      721 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
      781 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
      841 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
      901 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
      961 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     1021 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1081 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     1141 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     1201 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     1261 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     1321 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     1381 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     1441 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     1501 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     1561 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     1621 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     1681 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     1741 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     1801 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     1861 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     1921 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     1981 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     2041 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     2101 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     2161 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     2221 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     2281 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
     2341 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     2401 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     2461 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     2521 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
     2581 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     2641 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     2701 acgttgtaaa acgacggcca gtgaattgga tttaggtgac actata
//
