LOCUS       V015633                 6938 bp    DNA     circular SYN 14-OCT-2020
DEFINITION  Exported.
ACCESSION   V015633
VERSION     V015633
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6938)
  AUTHORS   Satanowski A, Dronsella B, Noor E, Voegeli B, He H, Wichmann P, Erb
            TJ, Lindner SN.
  TITLE     Awakening a latent carbon fixation cycle in Escherichia coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6938)
  AUTHORS   Satanowski A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-OCT-2020) Synthetic and Systems Metabolism Group, Max
            Planck Institute for Molecular Plant Physiology, Am Muehlenberg 1,
            Potsdam, Brandenburg 12163, Germany
REFERENCE   3  (bases 1 to 6938)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-OCT-2020) Synthetic and Systems Metabolism Group, Max Planck
            Institute for Molecular Plant Physiology, Am Muehlenberg 1, Potsdam,
            Brandenburg 12163, Germany"
FEATURES             Location/Qualifiers
     source          1..6938
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..39
                     /label="pgi20"
                     /note="pgi20"
                     /regulatory_class="promoter"
     regulatory      67..93
                     /label="spacer"
                     /note="spacer"
                     /regulatory_class="other"
     regulatory      94..109
                     /label="RBS 'C'"
                     /note="RBS 'C'"
                     /regulatory_class="ribosome_binding_site"
     CDS             113..130
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             131..1531
                     /gene="gnd"
                     /label="6-phosphogluconate dehydrogenase, decarboxylating"
                     /note="6-phosphogluconate dehydrogenase, decarboxylating
                     from Escherichia coli (strain K12). Accession#: P00350"
     regulatory      1547..1573
                     /label="spacer"
                     /note="spacer"
                     /regulatory_class="other"
     regulatory      1574..1589
                     /label="RBS 'C'"
                     /note="RBS 'C'"
                     /regulatory_class="ribosome_binding_site"
     CDS             1593..1610
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             1611..2246
                     /gene="eda"
                     /label="KHG/KDPG aldolase"
                     /note="KHG/KDPG aldolase from Escherichia coli (strain
                     K12). Accession#: P0A955"
     regulatory      2262..2288
                     /label="spacer"
                     /note="spacer"
                     /regulatory_class="other"
     regulatory      2289..2304
                     /label="RBS 'C'"
                     /note="RBS 'C'"
                     /regulatory_class="ribosome_binding_site"
     CDS             2308..2325
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             2326..4131
                     /gene="edd"
                     /label="Phosphogluconate dehydratase"
                     /note="Phosphogluconate dehydratase from Escherichia coli
                     (strain K12). Accession#: P0ADF6"
     promoter        4166..4269
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             4270..4926
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     terminator      5020..5106
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(5275..5820)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(5934..6028)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(6051..6839)
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     promoter        complement(6840..6931)
                     /label="AmpR promoter"
ORIGIN
        1 aatacttgac atatcactgt gattcacata taatatgcgc aggacgcact gaccgaattc
       61 actagttaat agaaataatt ttgtttaact ttaaagttaa gaggcaagaa tgcatcatca
      121 ccatcaccac tccaagcaac agatcggcgt agtcggtatg gcagtgatgg gacgcaacct
      181 tgcgctcaac atcgaaagcc gtggttatac cgtctctatt ttcaaccgtt cccgtgagaa
      241 gacggaagaa gtgattgccg aaaatccagg caagaaactg gttccttact atacggtgaa
      301 agagtttgtc gaatctctgg aaacgcctcg tcgcatcctg ttaatggtga aagcaggtgc
      361 aggcacggat gctgctattg attccctcaa accatatctc gataaaggag acatcatcat
      421 tgatggtggt aacaccttct tccaggacac tattcgtcgt aatcgtgagc tttcagcaga
      481 gggctttaac ttcatcggta ccggtgtttc tggcggtgaa gagggggcgc tgaaaggtcc
      541 ttctattatg cctggtggcc agaaagaagc ctatgaattg gtagcaccga tcctgaccaa
      601 aatcgccgcc gtagctgaag acggtgaacc atgcgttacc tatattggtg ccgatggcgc
      661 aggtcactat gtgaagatgg ttcacaacgg tattgaatac ggcgatatgc agctgattgc
      721 tgaagcctat tctctgctta aaggtggcct gaacctcacc aacgaagaac tggcgcagac
      781 ctttaccgag tggaataacg gtgaactgag cagttacctg atcgacatca ccaaagatat
      841 cttcaccaaa aaagatgaag acggtaacta cctggttgat gtgatcctgg atgaagcggc
      901 taacaaaggt accggtaaat ggaccagcca gagcgcgctg gatctcggcg aaccgctgtc
      961 gctgattacc gagtctgtgt ttgcacgtta tatctcttct ctgaaagatc agcgtgttgc
     1021 cgcatctaaa gttctctctg gtccgcaagc acagccagca ggcgacaagg ctgagttcat
     1081 cgaaaaagtt cgtcgtgcgc tgtatctggg caaaatcgtt tcttacgccc agggcttctc
     1141 tcagctgcgt gctgcgtctg aagagtacaa ctgggatctg aactacggcg aaatcgcgaa
     1201 gattttccgt gctggctgca tcatccgtgc gcagttcctg caaaaaatca ccgatgctta
     1261 tgccgaaaat ccacagatcg ctaacctgtt gctggctccg tacttcaagc aaattgccga
     1321 tgactaccag caggcgctgc gtgatgtcgt tgcttatgca gtacagaacg gtattccggt
     1381 tccgaccttc tccgcagcgg ttgcctatta cgacagctac cgtgctgctg ttctgcctgc
     1441 gaacctgatc caggcacagc gtgactattt tggtgcgcat acttataagc gtattgataa
     1501 agaaggtgtg ttccataccg aatggctgga ttaatctaga gctagttaat agaaataatt
     1561 ttgtttaact ttaaagttaa gaggcaagaa tgcatcatca ccatcaccac aaaaactgga
     1621 aaacaagtgc agaatcaatc ctgaccaccg gcccggttgt accggttatc gtggtaaaaa
     1681 aactggaaca cgcggtgccg atggcaaaag cgttggttgc tggtggggtg cgcgttctgg
     1741 aagtgactct gcgtaccgag tgtgcagttg acgctatccg tgctatcgcc aaagaagtgc
     1801 ctgaagcgat tgtgggtgcc ggtacggtgc tgaatccaca gcagctggca gaagtcactg
     1861 aagcgggtgc acagttcgca attagcccgg gtctgaccga gccgctgctg aaagctgcta
     1921 ccgaagggac tattcctctg attccgggga tcagcactgt ttccgaactg atgctgggta
     1981 tggactacgg tttgaaagag ttcaaattct tcccggctga agctaacggc ggcgtgaaag
     2041 ccctgcaagc gattgcgggt ccgttctccc aggtccgttt ctgcccgacg ggtggtattt
     2101 ctccggctaa ctaccgtgac tacctggcgc tgaaaagcgt gctgtgcatc ggtggttcct
     2161 ggctggttcc ggcagatgcg ctggaagcgg gcgattacga ccgcattact aagctggcgc
     2221 gtgaagctgt agaaggcgct aagctgtaat ctagagctag ttaatagaaa taattttgtt
     2281 taactttaaa gttaagaggc aagaatgcat catcaccatc accacaatcc acaattgtta
     2341 cgcgtaacaa atcgaatcat tgaacgttcg cgcgagactc gctctgctta tctcgcccgg
     2401 atagaacaag cgaaaacttc gaccgttcat cgttcgcagt tggcatgcgg taacctggca
     2461 cacggtttcg ctgcctgcca gccagaagac aaagcctctt tgaaaagcat gttgcgtaac
     2521 aatatcgcca tcatcacctc ctataacgac atgctctccg cgcaccagcc ttatgaacac
     2581 tatccagaaa tcattcgtaa agccctgcat gaagcgaatg cggttggtca ggttgcgggc
     2641 ggtgttccgg cgatgtgtga tggtgtcacc caggggcagg atggaatgga attgtcgctg
     2701 ctaagccgcg aagtgatagc gatgtctgcg gcggtggggc tgtcccataa catgtttgat
     2761 ggtgctctgt tcctcggtgt gtgcgacaag attgtcccgg gtctgacgat ggcagccctg
     2821 tcgtttggtc atttgcctgc ggtgtttgtg ccgtctggac cgatggcaag cggtttgcca
     2881 aataaagaaa aagtgcgtat tcgccagctt tatgccgaag gtaaagtgga ccgcatggcc
     2941 ttactggagt cagaagccgc gtcttaccat gcgccgggaa catgtacttt ctacggtact
     3001 gccaacacca accagatggt ggtggagttt atggggatgc agttgccagg ctcttctttt
     3061 gttcatccgg attctccgct gcgcgatgct ttgaccgccg cagctgcgcg tcaggttaca
     3121 cgcatgaccg gtaatggtaa tgaatggatg ccgatcggta agatgatcga tgagaaagtg
     3181 gtggtgaacg gtatcgttgc actgctggcg accggtggtt ccactaacca caccatgcac
     3241 ctggtggcga tggcgcgcgc ggccggtatt cagattaact gggatgactt ctctgacctt
     3301 tctgatgttg taccgctgat ggcacgtctc tacccgaacg gtccggccga tattaaccac
     3361 ttccaggcgg caggtggcgt accggttctg gtgcgtgaac tgctcaaagc aggcctgctg
     3421 catgaagatg tcaatacggt ggcaggtttt ggtctgtctc gttataccct tgaaccatgg
     3481 ctgaataatg gtgaactgga ctggcgggaa ggggcggaaa aatcactcga cagcaatgtg
     3541 atcgcttcct tcgaacaacc tttctctcat catggtggga caaaagtgtt aagcggtaac
     3601 ctgggccgtg cggttatgaa aacctctgcc gtgccggttg agaaccaggt gattgaagcg
     3661 ccagcggttg tttttgaaag ccagcatgac gttatgccgg cctttgaagc gggtttgctg
     3721 gaccgcgatt gtgtcgttgt tgtccgtcat caggggccaa aagcgaacgg aatgccagaa
     3781 ttacataaac tcatgccgcc acttggtgta ttattggacc ggtgtttcaa aattgcgtta
     3841 gttaccgatg gacgactctc cggcgcttca ggtaaagtgc cgtcagctat ccacgtaaca
     3901 ccagaagcct acgatggcgg gctgctggca aaagtgcgcg acggggacat cattcgtgtg
     3961 aatggacaga caggcgaact gacgctgctg gtagacgaag cggaactggc tgctcgcgaa
     4021 ccgcacattc ctgacctgag cgcgtcacgc gtgggaacag gacgtgaatt attcagcgcc
     4081 ttgcgtgaaa aactgtccgg tgccgaacag ggcgcaacct gtatcacttt ttaatctaga
     4141 gctagcatct ttctagagct agcgttgatc gggcacgtaa gaggttccaa ctttcaccat
     4201 aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag
     4261 gaagctaaaa tggagaaaaa aatcacggga tataccaccg ttgatatatc ccaatggcat
     4321 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt
     4381 cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg
     4441 gcctttattc acattcttgc ccgcctgatg aacgctcacc cggagtttcg tatggccatg
     4501 aaagacggtg agctggtgat ctgggatagt gttcaccctt gttacaccgt tttccatgag
     4561 caaactgaaa cgttttcgtc cctctggagt gaataccacg acgatttccg gcagtttctc
     4621 cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg
     4681 tttattgaga atatgttttt tgtctcagcc aatccctggg tgagtttcac cagttttgat
     4741 ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcacgatggg caaatattat
     4801 acgcaaggcg acaaggtgct gatgccgctg gcgatccagg ttcatcatgc cgtttgtgat
     4861 ggcttccatg tcggccgcat gcttaatgaa ttacaacagt attgtgatga gtggcagggc
     4921 ggggcgtaat aagctagcgc ggccgctcga gtatgtcgag tatgtcgagt atgtcgacct
     4981 gcagcccggg ggatcccatg gtacgcgtgc tagaggcatc aaataaaacg aaaggctcag
     5041 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg
     5101 acaaatccgc cgccctagac ctagggatat attccgcttc ctcgctcact gactcgctac
     5161 gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga acggggcgga gatttcctgg
     5221 aagatgccag gaagatactt aacagggaag tgagagggcc gcggcaaagc cgtttttcca
     5281 taggctccgc ccccctgaca agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa
     5341 cccgacagga ctataaagat accaggcgtt tccccctggc ggctccctcg tgcgctctcc
     5401 tgttcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc
     5461 cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac
     5521 cccccgttca gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     5581 aaagacatgc aaaagcacca ctggcagcag ccactggtaa ttgatttaga ggagttagtc
     5641 ttgaagtcat gcgccggtta aggctaaact gaaaggacaa gttttggtga ctgcgctcct
     5701 ccaagccagt tacctcggtt caaagagttg gtagctcaga gaaccttcga aaaaccgccc
     5761 tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa acgatctcaa
     5821 gaagatcatc ttattaatca gataaaatat tactagattt cagtgcaatt tatctcttca
     5881 aatgtagcac ctgaagtcag ccccatacga tataagttgt tactagtgct tggattctca
     5941 ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag
     6001 gtcattactg gatctatcaa caggagtcca agcgagctcg ttagacatta tttgccgact
     6061 accttggtga tctcgccttt cacgtagtgg acaaattctt ccaactgatc tgcgcgcgag
     6121 gccaagcgat cttcttcttg tccaagataa gcctgtctag cttcaagtat gacgggctga
     6181 tactgggccg gcaggcgctc cattgcccag tcggcagcga catccttcgg cgcgattttg
     6241 ccggttactg cgctgtacca aatgcgggac aacgtaagca ctacatttcg ctcatcgcca
     6301 gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat ttagcgcctc aaatagatcc
     6361 tgttcaggaa ccggatcaaa gagttcctcc gccgctggac ctaccaaggc aacgctatgt
     6421 tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga tcgtggctgg ctcgaagata
     6481 cctgcaagaa tgtcattgcg ctgccattct ccaaattgca gttcgcgctt agctggataa
     6541 cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt ctacagcgcg gagaatctcg
     6601 ctctctccag gggaagccga agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt
     6661 tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat cactgtgtgg cttcaggccg
     6721 ccatccactg cggagccgta caaatgtacg gccagcaacg tcggttcgag atggcgctcg
     6781 atgacgccaa ctacctctga tagttgagtc gatacttcgg cgatcaccgc ttccctcata
     6841 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     6901 atatttgaat gtatttagaa aaataaacaa atctcgag
//
