LOCUS       V006070                 7084 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006070
VERSION     V006070
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7084)
  AUTHORS   Dunn MR, Otto CE, Fenton KE, Chaput JC.
  TITLE     Transference as a Mechanism for Expanding and Improving Polymerase
            Function
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7084)
  AUTHORS   Dunn MR, Otto CE, Fenton KE, Chaput JC.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2015) The Biodesign Institute, Arizona State
            University, 727 E Tyler Street, Tempe, AZ 85287, USA
REFERENCE   3  (bases 1 to 7084)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7084)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-JAN-2015) The Biodesign Institute, Arizona State University, 727
            E Tyler Street, Tempe, AZ 85287, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..2325
                     /gene="pol"
                     /label="DNA polymerase"
                     /note="DNA polymerase from Pyrococcus glycovorans.
                     Accession#: Q9HH06"
     terminator      2364..2458
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             2502..3158
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     terminator      3226..3312
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    complement(3373..3394)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(3410..4489)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(4488..4565)
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     misc_feature    4757..4897
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5083..5671)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5845..6702)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6703..6807)
                     /label="AmpR promoter"
     promoter        6917..6961
                     /label="T5 promoter"
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     operon          6967..6987
                     /operon="LacO"
                     /label="LacO operon"
     protein_bind    6969..6985
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             7004..7015
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     CDS             7034..7051
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             7067..7084
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
ORIGIN
        1 atgatacttg acgctgacta catcaccgag gatgggaagc cgattataag gattttcaag
       61 aaagaaaacg gcgagtttaa ggttgagtac gacagaaact ttagacctta catttacgct
      121 ctcctcaaag atgactcgca gattgatgag gttaggaaga taaccgccga gaggcatggg
      181 aagatagtga gaattataga tgccgaaaag gtaaggaaga agttcctggg gaggccgatt
      241 gaggtatgga ggctgtactt tgaacaccct caggacgttc ccgcaataag ggataagata
      301 agagagcatt ccgcagttat tgacatcttt gagtacgaca ttccgttcgc gaagaggtac
      361 ctaatagaca aaggcctaat tccaatggaa ggcgatgaag agctcaagtt gctcgcattt
      421 gcgatcgcaa ccctctatca cgaaggggag gagttcgcga aggggcccat tataatgata
      481 agctatgctg atgaggaaga agccaaagtc ataacgtgga aaaagatcga tctcccgtac
      541 gtcgaggtag tttccagcga gagggagatg ataaagcggt tcctcaaggt gataagggag
      601 aaagatcccg atgttataat tacctacaac ggcgattctt tcgaccttcc ctatctagtt
      661 aagagggccg aaaagctcgg gataaagcta cccctgggaa gggacggtag tgagccaaag
      721 atgcagaggc ttggggatat gacagcggtg gagataaagg gaaggataca ctttgacctc
      781 taccacgtga ttaggagaac gataaacctc ccaacataca ccctcgaggc agtttatgag
      841 gcaatcttcg gaaagccaaa ggagaaagtt tacgctcacg agatagctga ggcctgggag
      901 actggaaagg gactggagag agttgcaaag tattcaatgg aggatgcaaa ggtaacgtac
      961 gagctcggta gggagttctt cccaatggag gcccagcttt caaggttagt cggccagccc
     1021 ctgtgggatg tttctaggtc ttcaactggc aacttggtgg agtggtacct cctcaggaag
     1081 gcctacgaga ggaatgaatt ggctccaaac aagccggatg agagggagta cgagagaagg
     1141 ctaagggaga gctacgctgg gggatacgtt aaggagccgg agaaagggct ctgggagggg
     1201 ttagtttccc tagatttcag gagcctgtac ccctcgataa taatcaccca taacgtctca
     1261 ccggatacgc tgaacaggga agggtgtagg gaatacgatg tcgccccaga ggttgggcac
     1321 aagttctgca aggacttccc ggggtttatc cccagcctgc tcaagaggtt attggatgaa
     1381 aggcaagaaa taaaaaggaa gatgaaagct tctaaagacc caatcgagaa gaagatgctt
     1441 gattacaggc aacgggcaat caaaatcctg gcgaattcct attatgggta ttatgggtac
     1501 gcaaaagccc gttggtactg taaggagtgc gcagagagcg ttacggcctg ggggagggaa
     1561 tatatagagt tcgtaaggaa ggaactggag gaaaagttcg ggttcaaagt cttatacata
     1621 gacacagatg gactctacgc cacaattcct ggggcaaaac ccgaggagat aaagaagaaa
     1681 gccctagagt tcgtagatta tataaacgcc aagctcccag ggctgttgga gcttgagtac
     1741 gagggcttct acgtgagagg gttcttcgtg acgaagaaga agtatgcgtt gatagatgag
     1801 gaagggaaga taatcactag ggggcttgaa atagtcagga gggactggag cgaaatagcc
     1861 aaagaaaccc aagcaaaagt cctagaggct atcctaaagc atggcaacgt tgaggaggca
     1921 gtaaagatag ttaaggaggt aactgaaaag ctgagcaagt acgaaatacc tccagaaaag
     1981 ctagttattt acgagcagat cacgaggccc cttcacgagt acaaggctat aggtccgcac
     2041 gttgccgtgg caaaaaggtt agccgctaga ggagtaaagg tgaggcctgg catggtgata
     2101 gggtacatag tgctgagggg agacgggcca ataagcaaga gggctatcct tgcagaggag
     2161 ttcgatctca ggaagcataa gtatgacgct gagtattaca tagaaaatca ggttttacct
     2221 gccgttctta gaatattaga ggcctttggg tacaggaaag aagacctcag gtggcagaag
     2281 actaaacaga caggtcttac ggcatggctt aacatcaaga agaagtaagt cgacctgcag
     2341 ccaagcttaa ttagctgagc ttggactcct gttgatagat ccagtaatga cctcagaact
     2401 ccatctggat ttgttcagaa cgctcggttg ccgccgggcg ttttttattg gtgagaatcc
     2461 aagctagctt ggcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg
     2521 gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
     2581 cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga
     2641 ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga
     2701 tgaatgctca tccggaattt cgtatggcaa tgaaagacgg tgagctggtg atatgggata
     2761 gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga
     2821 gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt
     2881 acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag
     2941 ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct
     3001 tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc
     3061 tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg
     3121 aattacaaca gtactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt
     3181 ggtgccctta aacgcctggg gtaatgactc tctagcttga ggcatcaaat aaaacgaaag
     3241 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
     3301 agtaggacaa atccgccgct ctagattacc gtgcagtcga tgataagctg tcaaacatga
     3361 gaattgtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     3421 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     3481 cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct
     3541 gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc
     3601 ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt
     3661 cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg gactcggtaa
     3721 tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga
     3781 tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt
     3841 cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac
     3901 gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca
     3961 atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt
     4021 tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt
     4081 ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt
     4141 gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg
     4201 acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg
     4261 acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg
     4321 ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt
     4381 tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat
     4441 aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcatt caccaccctg
     4501 aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgca ccattcgatg
     4561 gtgtcggaat ttcgggcagc gttgggtcct ggccacgggt gcgcatgatc tagagctgcc
     4621 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     4681 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     4741 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg
     4801 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     4861 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac
     4921 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     4981 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     5041 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     5101 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     5161 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     5221 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     5281 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     5341 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     5401 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     5461 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     5521 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     5581 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     5641 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     5701 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     5761 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     5821 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     5881 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     5941 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     6001 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     6061 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     6121 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     6181 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     6241 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     6301 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     6361 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     6421 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     6481 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     6541 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     6601 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     6661 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     6721 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     6781 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     6841 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     6901 tcttcacctc gagaaatcat aaaaaattta tttgctttgt gagcggataa caattataat
     6961 agattcaatt gtgagcggat aacaatttca cacagaattc attaaagagg agaaattaac
     7021 tatgagagga tctcaccatc accatcacca tacggatcca agcggcctgg tgccgcgcgg
     7081 cagc
//
