LOCUS       40924_21260        7081 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pGDR11-9N, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7081)
  AUTHORS   Dunn MR, Otto CE, Fenton KE, Chaput JC.
  TITLE     Transference as a Mechanism for Expanding and Improving Polymerase 
            Function
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7081)
  AUTHORS   Dunn MR, Otto CE, Fenton KE, Chaput JC.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2015) The Biodesign Institute, Arizona State 
            University, 727 E Tyler Street, Tempe, AZ 85287, USA
REFERENCE   3  (bases 1 to 7081)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7081)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-JAN-2015) The Biodesign Institute, Arizona State University, 727
            E Tyler Street, Tempe, AZ 85287, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7081
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..2325
                     /codon_start=1
                     /gene="pol"
                     /product="exonuclease-deficient DNA polymerase"
                     /label=pol
                     /note="from Thermococcus sp. 9oN-7"
                     /protein_id="ALL53331.1"
                     /translation="MILDTDYITENGKPVIRVFKKENGEFKIEYDRTFEPYFYALLKDD
                     SAIEDVKKVTAKRHGTVVKVKRAEKVQKKFLGRPIEVWKLYFNHPQDVPAIRDRIRAHP
                     AVVDIYEYDIPFAKRYLIDKGLIPMEGDEELTMLAFAIATLYHEGEEFGTGPILMISYA
                     DGSEARVITWKKIDLPYVDVVSTEKEMIKRFLRVVREKDPDVLITYNGDNFDFAYLKKR
                     CEELGIKFTLGRDGSEPKIQRMGDRFAVEVKGRIHFDLYPVIRRTINLPTYTLEAVYEA
                     VFGKPKEKVYAEEIAQAWESGEGLERVARYSMEDAKVTYELGREFFPMEAQLSRLIGQS
                     LWDVSRSSTGNLVEWFLLRKAYKRNELAPNKPDERELARRRGGYAGGYVKEPERGLWDN
                     IVYLDFRSLYPSIIITHNVSPDTLNREGCKEYDVAPEVGHKFCKDFPGFIPSLLGDLLE
                     ERQKIKRKMKATVDPLEKKLLDYRQRAIKILANSFYGYYGYAKARWYCKECAESVTAWG
                     REYIEMVIRELEEKFGFKVLYADTDGLHATIPGADAETVKKKAKEFLKYINPKLPGLLE
                     LEYEGFYVRGFFVTKKKYAVIDEEGKITTRGLEIVRRDWSEIAKETQARVLEAILKHGD
                     VEEAVRIVKEVTEKLSKYEVPPEKLVIHEQITRDLRDYKATGPHVAVAKRLAARGVKIR
                     PGTVISYIVLKGSGRIGDRAIPADEFDPTKHRYDAEYYIENQVLPAVERILKAFGYRKE
                     DLRYQKTKQVGLGAWLKVKGKK"
     gene            1..2325
                     /gene="pol"
                     /label=pol
     terminator      2361..2455
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             2499..3155
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      3223..3309
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    complement(3370..3391)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(3407..4486)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(4485..4562)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     misc_feature    4754..4894
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5080..5668)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5842..6699)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6700..6804)
                     /label=AmpR promoter
     promoter        6914..6958
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     operon          6964..6984
                     /operon="LacO"
                     /label=LacO operon
     protein_bind    6966..6982
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             7001..7012
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             7031..7048
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             7064..7081
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
ORIGIN
        1 atgattctgg ataccgatta cattaccgaa aacggcaaac cggtgattcg tgtgttcaaa
       61 aaagaaaacg gcgaattcaa aatcgaatac gatcgtacct ttgaaccgta tttttacgcg
      121 ctgctgaaag atgatagcgc gatcgaagat gtgaaaaaag tgaccgcgaa acgtcatggc
      181 accgtggtga aagtgaaacg tgcggaaaaa gtgcagaaaa aatttctggg ccgtccgatt
      241 gaagtgtgga aactgtattt caaccatccg caggatgtgc cggcgattcg tgatcgtatt
      301 cgtgcgcatc cggcggtggt ggatatttat gaatatgata tcccgttcgc gaaacgttat
      361 ctgattgata aaggcctgat tccgatggaa ggcgatgaag aactgaccat gctggccttt
      421 gcgattgcga ccctgtatca cgaaggcgaa gaatttggca ccggcccgat tctgatgatt
      481 agctatgcgg atggcagcga agcgcgtgtg attacctgga aaaaaatcga tctgccgtat
      541 gtggatgtgg tgagcaccga aaaagaaatg atcaaacgct ttctgcgtgt ggtgcgtgaa
      601 aaagatccgg atgtgctgat tacctataac ggcgataact ttgatttcgc gtatctgaaa
      661 aaacgttgcg aagaactggg catcaaattt accctgggcc gtgatggtag cgaaccgaaa
      721 attcagcgta tgggcgatcg ttttgcggtg gaagtgaaag gccgtattca ttttgatctg
      781 tatccggtga ttcgccgtac cattaacctg ccgacctata ccctggaagc ggtgtatgaa
      841 gcggtgtttg gcaaaccgaa agaaaaagtg tacgcggaag aaattgcgca ggcgtgggaa
      901 agcggcgaag gcctggaacg tgtggcgcgt tatagcatgg aagatgcgaa agtgacctat
      961 gaactgggcc gtgaattttt cccgatggaa gcgcagctgt ctcgtctgat tggccagagc
     1021 ctgtgggatg tgagccgtag cagcaccggc aacctggtgg aatggtttct gctgcgtaaa
     1081 gcgtataaac gtaacgaact ggccccgaac aaaccggatg aacgtgaact ggcccgtcgt
     1141 cgtggcggtt atgcgggcgg ttatgtgaaa gaaccggaac gtggcctgtg ggataacatt
     1201 gtgtatctgg attttcgtag cctgtatccg agcattatta tcacccataa cgtgagcccg
     1261 gataccctga accgtgaagg ctgcaaagaa tatgatgtgg cgccggaagt gggccataaa
     1321 ttctgcaaag atttcccggg ctttattccg agcctgctgg gcgatctgct ggaagaacgc
     1381 cagaaaatca aacgcaaaat gaaagcgacc gttgatccgc tggaaaaaaa actgctggat
     1441 tatcgtcagc gcgcgattaa aattctggcc aacagcttct atggctatta tggttatgcg
     1501 aaagcgcgtt ggtattgcaa agaatgcgcg gaaagcgtga ccgcgtgggg ccgtgaatat
     1561 atcgaaatgg tgatccgcga actggaagaa aaattcggct tcaaagtgct gtatgcggat
     1621 accgatggcc tgcatgcgac cattccgggt gcggatgcgg aaaccgtgaa aaaaaaagcg
     1681 aaagaattcc tgaaatacat caatccgaaa ctgccgggcc tgctggaact ggaatatgaa
     1741 ggcttttatg tgcgtggctt tttcgtgacc aaaaaaaaat acgcggtgat cgatgaagaa
     1801 ggcaaaatta ccacccgtgg cctggaaatt gtgcgtcgtg attggagcga aattgcgaaa
     1861 gaaacccagg cgcgtgtgct ggaagcgatt ctgaaacatg gcgatgtgga agaagcggtg
     1921 cgtattgtta aagaagtgac cgaaaaactg agcaaatatg aagttccgcc ggaaaaactg
     1981 gtgattcatg aacaaattac ccgtgatctg cgtgattata aagcgaccgg tccgcatgtg
     2041 gcggtggcaa aacgtctggc agcgcgtggc gtgaaaattc gtccgggcac cgtgattagc
     2101 tatattgtgc tgaaaggcag cggccgtatt ggcgatcgtg cgattccggc ggatgaattt
     2161 gatccgacca aacatcgtta tgatgcggaa tattatatcg aaaaccaggt gctgccggcg
     2221 gtggaacgta ttctgaaagc gtttggctat cgtaaagaag atctgcgcta tcagaaaacc
     2281 aaacaggtgg gcctgggcgc gtggctgaaa gttaaaggca aaaaagtcga cctgcagcca
     2341 agcttaatta gctgagcttg gactcctgtt gatagatcca gtaatgacct cagaactcca
     2401 tctggatttg ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaatccaag
     2461 ctagcttggc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
     2521 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
     2581 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
     2641 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
     2701 atgctcatcc ggaatttcgt atggcaatga aagacggtga gctggtgata tgggatagtg
     2761 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
     2821 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
     2881 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
     2941 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
     3001 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
     3061 cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat
     3121 tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc agttattggt
     3181 gcccttaaac gcctggggta atgactctct agcttgaggc atcaaataaa acgaaaggct
     3241 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
     3301 aggacaaatc cgccgctcta gattaccgtg cagtcgatga taagctgtca aacatgagaa
     3361 ttgtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     3421 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     3481 tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat
     3541 tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca
     3601 gcaggcgaaa atcctgtttg atggtggtta acggcgggat ataacatgag ctgtcttcgg
     3661 tatcgtcgta tcccactacc gagatatccg caccaacgcg cagcccggac tcggtaatgg
     3721 cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc
     3781 cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc
     3841 gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca
     3901 gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg tgacccaatg
     3961 cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata atactgttga
     4021 tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca
     4081 cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg acgcgttgcg
     4141 cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca
     4201 ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg
     4261 gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt ttgcccgcca
     4321 gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt
     4381 cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag
     4441 agacaccggc atactctgcg acatcgtata acgttactgg tttcattcac caccctgaat
     4501 tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcacca ttcgatggtg
     4561 tcggaatttc gggcagcgtt gggtcctggc cacgggtgcg catgatctag agctgcctcg
     4621 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     4681 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     4741 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct
     4801 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     4861 gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga
     4921 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     4981 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     5041 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     5101 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     5161 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     5221 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     5281 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     5341 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     5401 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     5461 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     5521 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     5581 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     5641 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     5701 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     5761 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     5821 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     5881 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     5941 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     6001 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     6061 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     6121 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     6181 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     6241 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     6301 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     6361 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     6421 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     6481 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     6541 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     6601 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     6661 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     6721 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     6781 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     6841 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     6901 tcacctcgag aaatcataaa aaatttattt gctttgtgag cggataacaa ttataataga
     6961 ttcaattgtg agcggataac aatttcacac agaattcatt aaagaggaga aattaactat
     7021 gagaggatct caccatcacc atcaccatac ggatccaagc ggcctggtgc cgcgcggcag
     7081 c
//
