LOCUS       V006072                 4591 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006072
VERSION     V006072
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Stokes JM, MacNair CR, Ilyas B, French S, Cote J-P., Farha MA,
            Sieron AO, Coombes BK, Brown ED.
  TITLE     An antiprotozoal drug overcomes acquired colistin resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   Stokes JM, MacNair CR, Ilyas B, French S, Cote J-P., Farha MA,
            Sieron AO, Coombes BK, Brown ED.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2016) Biochemistry and Biomedical Sciences,
            McMaster University, 1200 Main Street West, Hamilton, Ontario L8N
            3Z5, Canada
REFERENCE   3  (bases 1 to 4591)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4591)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (16-SEP-2016) Biochemistry and Biomedical Sciences, McMaster
            University, 1200 Main Street West, Hamilton, Ontario L8N 3Z5,
            Canada"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4591
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             150..172
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             179..1801
                     /gene="mcr1"
                     /label="Probable phosphatidylethanolamine transferase
                     Mcr-1"
                     /note="Probable phosphatidylethanolamine transferase Mcr-1
                     from Escherichia coli. Accession#: A0A0R6L508"
     CDS             1811..1828
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      complement(1890..1917)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     CDS             complement(2011..2823)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      2945..3533
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(3719..3861)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(3966..4154)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
ORIGIN
        1 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg
       61 cataagggag agcgtcgaga tcccggacac catcgaatgg cgcaaaacct ttcgcggtat
      121 ggcatgatag cgcccggtct agaaataatt ttgtttaact ttaagaagga gatataccat
      181 ggtccagcat acttctgtgt ggtaccgacg ctcggtcagt ccgtttgttc ttgtggcgag
      241 tgttgccgtt ttcttgaccg cgaccgccaa tcttaccttt tttgataaaa tcagccaaac
      301 ctatcccatc gcggacaatc tcggctttgt gctgacgatc gctgtcgtgc tctttggcgc
      361 gatgctactg atcaccacgc tgttatcatc gtatcgctat gtgctaaagc ctgtgttgat
      421 tttgctatta atcatgggcg cggtgaccag ttattttact gacacttatg gcacggtcta
      481 tgatacgacc atgctccaaa atgccctaca gaccgaccaa gccgagacca aggatctatt
      541 aaacgcagcg tttatcatgc gtatcattgg tttgggtgtg ctaccaagtt tgcttgtggc
      601 ttttgttaag gtggattatc cgacttgggg caagggtttg atgcgccgat tgggcttgat
      661 cgtggcaagt cttgcgctga ttttactgcc tgtggtggcg ttcagcagtc atatggccag
      721 tttctttcgc gtgcataagc cgctgcgtag ctatgtcaat ccgatcatgc caatctactc
      781 ggtgggtaag cttgccagta ttgagtataa aaaagccagt gcgccaaaag ataccattta
      841 tcacgccaaa gacgcggtac aagcaaccaa gcctgatatg cgtaagccac gcctagtggt
      901 gttcgtcgtc ggtgagacgg cacgcgccga tcatgtcagc ttcaatggct atgagcgcga
      961 tactttccca cagcttgcca agatcgatgg cgtgaccaat tttagcaatg tcacatcgtg
     1021 cggcacatcg acggcgtatt ctgtgccgtg tatgttcagc tatctgggcg cggatgagta
     1081 tgatgtcgat accgccaaat accaagaaaa tgtgctggat acgctggatc gcttgggcgt
     1141 aagtatcttg tggcgtgata ataattcgga ctcaaaaggc gtgatggata agctgccaaa
     1201 agcgcaattt gccgattata aatccgcgac caacaacgcc atctgcaaca ccaatcctta
     1261 taacgaatgc cgcgatgtcg gtatgctcgt tggcttagat gactttgtcg ctgccaataa
     1321 cggcaaagat atgctgatca tgctgcacca aatgggcaat cacgggcctg cgtattttaa
     1381 gcgatatgat gaaaagtttg ccaaattcac gccagtgtgt gaaggtaatg agcttgccaa
     1441 gtgcgaacat cagtccttga tcaatgctta tgacaatgcc ttgcttgcca ccgatgattt
     1501 catcgctcaa agtatccagt ggctgcagac gcacagcaat gcctatgatg tctcaatgct
     1561 gtatgtcagc gatcatggcg aaagtctggg tgagaacggt gtctatctac atggtatgcc
     1621 aaatgccttt gcaccaaaag aacagcgcag tgtgcctgca tttttctgga cggataagca
     1681 aactggcatc acgccaatgg caaccgatac cgtcctgacc catgacgcga tcacgccgac
     1741 attattaaag ctgtttgatg tcaccgcgga caaagtcaaa gaccgcaccg cattcatccg
     1801 ctgactcgag caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga
     1861 agctgagttg gctgctgcca ccgctgagca aaaggccatc cgtcaggatg gccttctggt
     1921 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     1981 aatatgtatc cgctcatgaa ttaattctta gaaaaactca tcgagcatca aatgaaactg
     2041 caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga
     2101 aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat
     2161 tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc
     2221 aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa gtttatgcat
     2281 ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc
     2341 aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt
     2401 aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc
     2461 aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg ttttcccggg
     2521 gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg
     2581 aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc
     2641 aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg
     2701 atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc
     2761 agcatccatg ttggaattta atcgcggcct agagcaagac gtttcccgtt gaatatggct
     2821 cataacaccc cttgtattac tgtttatgta agcagacagt tttattgttc atgaccaaaa
     2881 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2941 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3001 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3061 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     3121 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3181 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3241 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3301 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3361 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3421 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3481 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3541 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3601 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3661 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3721 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac
     3781 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta
     3841 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     3901 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     3961 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca
     4021 gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt
     4081 ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt
     4141 tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata
     4201 ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta
     4261 ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc
     4321 actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag
     4381 cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc
     4441 agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt
     4501 ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta
     4561 aggcaacccc gccagcctag ccgggagatc t
//
