LOCUS       40924_21250        9016 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGDHFluc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9016)
  AUTHORS   Barash NR, Nosala C, Pham JK, McInally SG, Gourguechon S, 
            McCarthy-Sinclair B, Dawson SC.
  TITLE     Giardia colonizes and encysts in high density foci in the murine 
            small intestine
  JOURNAL   mSphere (2017) In press
REFERENCE   2  (bases 1 to 9016)
  AUTHORS   Barash NR, Nosala C, Pham JK, McInally SG, Gourguechon S, 
            McCarthy-Sinclair B, Dawson SC.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2017) Department of Microbiology and Molecular 
            Genetics, Uc Davis, One Shields Avenue, Davis, CA 95616, USA
REFERENCE   3  (bases 1 to 9016)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9016)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mSphere 
            (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-MAY-2017) Department of Microbiology and Molecular Genetics, Uc 
            Davis, One Shields Avenue, Davis, CA 95616, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9016
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      17..605
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    893..914
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        929..959
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    967..983
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     991..1007
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1025..1043
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     5'UTR           1050..1101
                     /gene="GDH"
     CDS             1104..2753
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(2800..2921)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     3'UTR           3041..3162
                     /gene="alpha-2 tubulin"
     gene            3041..3162
                     /gene="alpha-2 tubulin"
                     /label=alpha-2 tubulin
     5'UTR           3241..3440
                     /gene="gamma giardin"
     gene            3241..3440
                     /gene="gamma giardin"
                     /label=gamma giardin
     CDS             3450..4043
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     3'UTR           4053..4171
                     /gene="GDH"
     misc_feature    complement(4176..4892)
                     /label=similar to aurora kinase
                     /note="similar to aurora kinase"
     CDS             complement(5624..5635)
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     gene            complement(6747..6919)
                     /gene="NOP5"
                     /label=NOP5
     promoter        complement(6928..6946)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6953..6969)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(7110..7565)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7897..8001
                     /label=AmpR promoter
     CDS             8002..8859
                     /label=AmpR
                     /note="beta-lactamase"
ORIGIN
        1 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
       61 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      121 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      181 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      241 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      301 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      361 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      421 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      481 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      541 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      601 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      661 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      721 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      781 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
      841 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
      901 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
      961 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     1021 agctatttag gtgacactat agaatactca agcttgacca caaataacgc ctttaattac
     1081 aggcgcccca gattttaaaa tccatggaag atgccaaaaa cattaagaag ggcccagcgc
     1141 cattctaccc actcgaagac gggaccgccg gcgagcagct gcacaaagcc atgaagcgct
     1201 acgccctggt gcccggcacc atcgccttta ccgacgcaca tatcgaggtg gacattacct
     1261 acgccgagta cttcgagatg agcgttcggc tggcagaagc tatgaagcgc tatgggctga
     1321 atacaaacca tcggatcgtg gtgtgcagcg agaatagctt gcagttcttc atgcccgtgt
     1381 tgggtgccct gttcatcggt gtggctgtgg ccccagctaa cgacatctac aacgagcgcg
     1441 agctgctgaa cagcatgggc atcagccagc ccaccgtcgt attcgtgagc aagaaagggc
     1501 tgcaaaagat cctcaacgtg caaaagaagc taccgatcat acaaaagatc atcatcatgg
     1561 atagcaagac cgactaccag ggcttccaaa gcatgtacac cttcgtgact tcccatttgc
     1621 cacccggctt caacgagtac gacttcgtgc ccgagagctt cgaccgggac aaaaccatcg
     1681 ccctgatcat gaacagtagt ggcagtaccg gattgcccaa gggcgtagcc ctaccgcacc
     1741 gcaccgcttg tgtccgattc agtcatgccc gcgaccccat cttcggcaac cagatcatcc
     1801 ccgacaccgc tatcctcagc gtggtgccat ttcaccacgg cttcggcatg ttcaccacgc
     1861 tgggctactt gatctgcggc tttcgggtcg tgctcatgta ccgcttcgag gaggagctat
     1921 tcttgcgcag cttgcaagac tataagattc aatctgccct gctggtgccc acactattta
     1981 gcttcttcgc taagagcact ctcatcgaca agtacgacct aagcaacttg cacgagatcg
     2041 ccagcggcgg ggcgccgctc agcaaggagg taggtgaggc cgtggccaaa cgcttccacc
     2101 taccaggcat ccgccagggc tacggcctga cagaaacaac cagcgccatt ctgatcaccc
     2161 ccgaagggga cgacaagcct ggcgcagtag gcaaggtggt gcccttcttc gaggctaagg
     2221 tggtggactt ggacaccggt aagacactgg gtgtgaacca gcgcggcgag ctgtgcgtcc
     2281 gtggccccat gatcatgagc ggctacgtta acaaccccga ggctacaaac gctctcatcg
     2341 acaaggacgg ctggctgcac agcggcgaca tcgcctactg ggacgaggac gagcacttct
     2401 tcatcgtgga ccggctgaag agcctgatca aatacaaggg ctaccaggta gccccagccg
     2461 aactggagag catcctgctg caacacccca acatcttcga cgccggggtc gccggcctgc
     2521 ccgacgacga tgccggcgag ctgcccgccg cagtcgtcgt gctggaacac ggtaaaacca
     2581 tgaccgagaa ggagatcgtg gactatgtgg ccagccaggt tacaaccgcc aagaagctgc
     2641 gcggtggtgt tgtgttcgtg gacgaggtgc ctaaaggact gaccggcaag ttggacgccc
     2701 gcaagatccg cgagattctc attaaggcca agaagggcgg caagatcgcc gtgtaataat
     2761 tctagagtcg gggcggccgg ccgcttcgag cagacatgat aagatacatt gatgagtttg
     2821 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     2881 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     2941 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
     3001 acaaatgtgg taaaatcgat aaggatctgg cggccgcagt gaattctttc cgtcgacctt
     3061 tgcatcatcg ttgctcggtc gtctgcctcg tttacgacct gcttgttcct gggcctcaaa
     3121 tggttagtgg ttttccttct tccaacggcg ttggtccatc ctcggccgcg tgatcaaatg
     3181 ccttcgagcg tatgtctgac ctgtttgtga atgacgatcc gtggctttac tcagattcct
     3241 gtgtttgtga ataagaaatc cgtgaatttg ttggggtgga acggcgaagt ggcctctgct
     3301 acaataagaa ctggagagtg tgttcagagg cgaatgcgca acgaactttg tcgttacacg
     3361 acgtgtgcgc ctgtggtctt cgtgcggttt ccgcgcggaa ttttgcgcct tttctaaaaa
     3421 actaacccta aaacaagaaa atcatgggca ccgagtacaa gcccacggtg cgcctcgcca
     3481 cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     3541 ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     3601 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     3661 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     3721 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     3781 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     3841 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     3901 agcgcgccgg ggtgcctgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     3961 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     4021 gcatgacccg caagcccggt gccctcgagt gagggctgaa gtgaatattt accttttccg
     4081 ggttcgacgg gcgtgcttga cgatgcggaa gtcgctgtcc gttccaacgg ctatctgccc
     4141 caggaccccc atcccggcgc cgatcagctg gtaccgagct catagcacag ggctccgagt
     4201 gaccagatat cgcaagcctt atcataagat tgtccgagca tgacctccgg gggaaggtag
     4261 tcgagagttc cgcactgggt gcgtcgtcgg ccaccgtggg ttggatggtg cacggaccat
     4321 ccaaagtctg caatcttgta tgtgtagtgt gccattcgga ctagctcgtg ttcatgcaca
     4381 gaaatggagt catctgaatt gtgcgcatct gtgtgctctt tggtctcttt ccccttgcaa
     4441 atcaagatgt tttctgcctt aaggtcccta tggaagatgt tctttctgtg aagatactgt
     4501 atcgcctgtg ccacgtctcg aataatcttg cttacaacgg gtagaggaaa cttcccgaac
     4561 tgctgtaacc tttggtaaag ctcgccgtac ggagccactt cgaggaccaa aaagactcgg
     4621 gtctcgtcct ggaaccaagt atacaagcga attatgttta tgtgcctcac attaagatgt
     4681 atatccaatt ccctgcgcac ctggccctca acacgctccg atttgatgta ggatttatac
     4741 agcactttaa gggcaaccac caacttggag gactgttcac gtgctaggta aaccaagcca
     4801 tactttccac gaccaagaaa gcgtccaatc tcaaagtcct ctattgtagt gcgctttcct
     4861 gtccctgtgt ggggtaccaa gtgctgcggc attttttaag ttatttactt ggagtgcgtc
     4921 ttgttcttct tatcgtcatc cttgctctta tgcttttcct tgtctttttc tctgtcttta
     4981 tccctgtgct tgtccttgtc tttatgtttt tccttatctc tttctttatc cttatccttg
     5041 tccctgtgct tctctttatc cttctccttc tccttgtgct tttctttttc tttgtccttg
     5101 tctctgtgct tttccttgct cttctcctta tgcttctcct tagcgtcctt ggtgtcattg
     5161 gtgtcttccg catccacctc aattggtgcc acgggctcgt ctttcttcgc agcactcgat
     5221 aaaggctcta agggcgctgg catagcatcg tcttgtcgtt cttgtgcatt ctccttattt
     5281 cttgagccct tatcctgtga ggccttggtc tgcgtgaacc cagtgtcttc ctcaaacgcc
     5341 tccgtcattc gagtcttttt gggctcagca gcaggggctg tttccttcga aagcttcatg
     5401 tcccccttcc cttcatatcc tgttgccact ccgcgcctgt atgcttccat cttctgtgaa
     5461 gcaatagatt ttctgtttga gcgagcttgc ttgtcccctt catagaagcg cagagacgcg
     5521 tttatcttct caagctttcc tttagcaaag ctggtgttag aggactccga gagagcgtcc
     5581 acacgagcag caagagatgc gtttgttgct acgacacgtg ccatcttccc tcgattggtt
     5641 gcagatgctt ttcccacaag cgcagcgtga tagataaacc catatttggg cgtctgcttg
     5701 gatcgcattt tcttggcacg gaaaagtgca cgttctgagc caagaatctg caaggtggat
     5761 gcggggagct tggcaaggtt gagcaaactt cccgctttgg ccagaagacg gacgccgacg
     5821 atggacccga caatgcgggt gaaattgggt gcaacagcaa gcatgcgtgc ctcaatgtac
     5881 tcatgcaact cctcgcggta tgagaccaaa gaaagaagct cctcagccag gtggacaact
     5941 ctgtcggagt cctggtcagt aatatccgtg ccaacagatg tcttagcagc agatataatg
     6001 gcataagctt gatccttaga cgctttgtta agggccttta tgaacttttc gcggccagac
     6061 gttgtgagcg actcctcgag atgcctcctg tcccggagca cgactacagt cctggcatag
     6121 tctatattat ctggtacgat agtaatcagc tcggggaagt gccaaaccat ccactcacga
     6181 agtcgcatgg caaactggtt gatatccttc tcagtgtcgt caattaaagc aatggcttga
     6241 ataagcatga tatctataga gtcagcattg aacttgacgc ggtagcgaga aaaggagtgt
     6301 gcaagcccca tcgccattga gctgtcgtgt tcttgatcaa aatcagggag aatggaggcc
     6361 agatgcgtac gtatccccct agacagctca acaactatgt cattaaatgc agcacggacc
     6421 ccgtagacat ccttgagggc ggtaaccatg tgagggtcct ggaccaggag cttttcggac
     6481 gcatccttct tggctacggt gttcacaaag tgtttctcca caaacttttt tagctcctcg
     6541 ctcacatcct tctcaaaggt tgaggtttgt gtggctaacg caccctcgac gcttggaaac
     6601 tttgaaaatg agacaaggga aactattttc tggacgtcac tacttgtgcg catcattgcc
     6661 ccgaccaggt cggcggcctt aagcttgctt tcgtccaaaa ccttgaacat cgctaagcca
     6721 gacgcggcct catataacag gtacatttaa taaaaacaga aatccacgct tgggcattta
     6781 ttgcaaatat tattttacta atgacaattt atggtaaatt gccaacacta cagcctcagc
     6841 agactctatc cccagggttt gcaacgcagt gaaatggctg acaggggctt agctgtgcgc
     6901 tcaccaccaa agcgagctcg aattcgccct atagtgagtc gtattacaat tcactggccg
     6961 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     7021 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     7081 aacagttgcg cagcctgaat ggcgaatgga aattgtaaac gttaatattt tgttaaaatt
     7141 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     7201 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     7261 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     7321 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     7381 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     7441 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     7501 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     7561 cgcgtcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     7621 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     7681 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     7741 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     7801 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     7861 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     7921 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     7981 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     8041 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     8101 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     8161 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     8221 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     8281 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     8341 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     8401 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     8461 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     8521 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     8581 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     8641 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     8701 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     8761 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     8821 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     8881 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     8941 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     9001 tcagaccccg tagaaa
//
