LOCUS       40924_21240        6773 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGCD19, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6773)
  AUTHORS   Dantas GC, Ferreira H.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAR-2014) Bioquimica e Microbiologia, UNESP, Av. 24A, 
            1515, Rio Claro, SP 13506900, Brazil
REFERENCE   2  (bases 1 to 6773)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6773)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (25-MAR-2014) Bioquimica e Microbiologia, UNESP, Av. 24A, 1515, Rio 
            Claro, SP 13506900, Brazil"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6773
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             18..731
                     /codon_start=1
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1595..1611)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1752..2180
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             2524..3315
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3336..4193
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4367..4955
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5243..5264
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5279..5309
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5317..5333
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5341..5357
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(5373..6425)
                     /codon_start=1
                     /gene="xylR"
                     /product="xylose promoter repressor"
                     /label=xylR
                     /note="XylR"
                     /protein_id="AIC38375.1"
                     /translation="MTGLNKSTVSSQVNTLMKESMVFEIGQGQSSGGRRPVMLVFNKKA
                     GYSVGIDVGVDYINGILTDLEGTIVLDQYRHLESNSPEITKDILIDMIHHFITQMPQSP
                     YGFIGIGICVPGLIDKDQKIVFTPNSNWRDIDLKSSIQEKYNVSVFIENEANAGAYGEK
                     LFGAAKNHDNIIYVSISTGIGIGVIINNHLYRGVSGFSGEMGHMTIDFNGPKCSCGNRG
                     CWELYASEKALLKSLQTKEKKLSYQDIINLAHLNDIGTLNALQNFGFYLGIGLTNILNT
                     FNPQAVILRNSIIESHPMVLNSMRSEVSSRVYSQLGNSYELLPSSLGQNAPALGMSSIV
                     IDHFLDMITM"
     gene            complement(5373..6425)
                     /gene="xylR"
                     /label=xylR
ORIGIN
        1 gatccatggc actcgagatg agtaaaggag aagaactttt cactggagtt gtcccaattc
       61 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag
      121 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg
      181 ttccatggcc aacacttgtc actactttcg cgtatggtct tcaatgcttt gcgagatacc
      241 cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg
      301 aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg
      361 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa
      421 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag
      481 acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa
      541 gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt
      601 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga
      661 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg
      721 aactatacaa agaagcaggc ggccgcgcac tgaatctaga atgacccggg agctgctgca
      781 tgctggcgcc aatggcgaac gcgttgcgca ccgcattgaa cagcggaaag tcgtaggccg
      841 catgcggcgt ggattgcagg tacggcgcca ggaactggtc gtaatcgcca ttgcccgtac
      901 cgccgccggt gatcacttcg ccgaagacat acacacccga tcggatgccg gcatcgaaga
      961 cgcgattgag atggtcgaac gtcatgtgct tggccgcatc cacccggaag ccggtcacgc
     1021 ccatgctctt gagcgcctgc agataggcgc gctgctgctg taccacccag tcgttgccca
     1081 gcaaatccgg caggcctgga tcgctgccgc cgccgcagat acggtaattg cgcacctggt
     1141 aagcgtcgtt gtagttgctg atgcactgcg ccgggccgaa atcgctggcg ctgaggaagt
     1201 ttgactgcag cgtgccgaac aagcgcagcg aatcgtaacg gccggggttt gccgcgtact
     1261 gcgacaggac cgcgccgcct gggtaattga ggtccgagcg tgtggccgct tcgttggcca
     1321 tgtggttgaa cacgatgtcg gcataggttt ccacgccgtt gctggccagc gcctggacca
     1381 tcttcttgaa tgcagcggtg tcgccgagcg gattgtcgat caggcggatg tcctggggtt
     1441 gatagcgcgc ccaccaggca cttccttcgg agcggtacgc gggggcgacc aataccttgc
     1501 gatagccggc atcggcaatc tgcttggcgc gcgcttccac ggtggcatac ggccagttga
     1561 aggcgtgcag gatgacatcc ccgggaagct tggcactggc cgtcgtttta caacgtcgtg
     1621 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     1681 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     1741 atggcgaatg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     1801 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     1861 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     1921 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     1981 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     2041 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     2101 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     2161 acaaaaattt aacgcgaatt ttaacaaaat tcagggcgca agggctgcta aaggaagcgg
     2221 aacacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt cagctactgg
     2281 gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg cagtgggctt
     2341 acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga attgccagct
     2401 ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc tttcttgccg
     2461 ccaaggatct gatggcgcag gggatcaaga tctgatcaag agacaggatg aggatcgttt
     2521 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     2581 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     2641 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     2701 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     2761 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     2821 caggatctcc tgtcatccca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     2881 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     2941 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     3001 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     3061 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     3121 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     3181 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     3241 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     3301 cttgacgagt tcttctgaat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     3361 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     3421 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     3481 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     3541 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     3601 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     3661 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     3721 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     3781 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     3841 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     3901 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     3961 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     4021 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     4081 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     4141 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     4201 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     4261 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     4321 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     4381 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     4441 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     4501 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     4561 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     4621 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     4681 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     4741 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     4801 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     4861 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     4921 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     4981 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     5041 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     5101 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     5161 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     5221 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     5281 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     5341 caggaaacag ctatgaccat gattacgaat tcttacattg taatcatgtc cagaaaatga
     5401 tcaatcacaa tggaggacat tcctaatgcc ggtgcattct gtcctaagga agatggcaat
     5461 aattcatagc tattgcctaa ttgggaataa acccttgatg atacttcact tctcattgaa
     5521 tttaaaacca taggatgcga ttcaattatg ctatttctta aaattacggc ttgtgggttg
     5581 aaagtattta gaatattggt aaggcctatt cctaaataga atccaaaatt ttgtaatgca
     5641 tttaaggttc cgatatcatt cagatgggcg aggtttatga tatcttgata ggacagtttt
     5701 ttctctttgg tctgaagaga ttttaataaa gccttctctg aagcatacaa ttcccagcat
     5761 cctcggtttc cgcaactgca tttaggacca ttaaagtcta ttgtcatatg tcccatttct
     5821 ccagagaagc cgcttactcc tctatataaa tgattgttga taataacacc gatccctatt
     5881 cctgtgctga tacttacgta aataatgtta tcgtgatttt ttgcagctcc aaatagtttt
     5941 tctccatatg cgccagcatt tgcctcattt tcaataaaaa cagacacatt gtacttctct
     6001 tgtatcgaag attttaagtc aatatctctc cagttggagt tcggagtgaa aacaattttt
     6061 tgatctttat caatgagtcc aggcacgcaa atacctatac caataaaccc gtacggagat
     6121 tggggcattt gcgtaataaa gtgatgaatc atatcaatca aaatgtcttt cgttatttct
     6181 ggagaattgg attccaaatg gcggtattga tcaagaacga ttgttccttc aaggtctgtt
     6241 aaaatgccat taatataatc cacaccaaca tctattccaa cggagtatcc tgccttttta
     6301 ttaaaaacaa gcatgacagg tcttcttccg ccacttgatt gtccttgacc tatttcaaat
     6361 accatacttt ctttcattaa cgtgtttacc tgtgatgaga cagttgattt atttaatcca
     6421 gtcatttcag ataattttgc tcttgaaata ggtgaatttt taaggatttc ttttaataat
     6481 aacttttgat ttactttttt gacaaaggtt tgatcagcga tatccacttc atccactcca
     6541 tttgtttaat ctttaaatta agtatcaaca tagtacatag cgaatcttcc ctttattaaa
     6601 tctaatgtgt tcataaaaaa ctaaaaaaaa tattgaaaat actgacgagg ttatataaga
     6661 tgaaaataag ttagtttgtt taaacaacaa actaataggt gatgtactta ctatatgaaa
     6721 taaaatgcat ctgtatttga atgaatttat taagcttggg aaaaggaggt gag
//
