LOCUS       V006078                 6411 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006078
VERSION     V006078
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6411)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6411)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6411)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6411)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             7..894
                     /gene="Myd88"
                     /label="Myeloid differentiation primary response protein
                     MyD88"
                     /note="Myeloid differentiation primary response protein
                     MyD88 from Mus musculus. Accession#: P22366"
     terminator      925..1112
                     /label="ADH1 terminator"
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(1168..1839)
                     /label="TRP1"
                     /note="phosphoribosylanthranilate isomerase, required for
                     tryptophan biosynthesis"
     promoter        complement(1840..1941)
                     /label="TRP1 promoter"
     primer_bind     complement(1956..1972)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1980..1996)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2004..2034)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2049..2070)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2358..2946)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3120..3977)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3978..4082)
                     /label="AmpR promoter"
     rep_origin      4364..5528
                     /label="2u ori"
                     /note="yeast 2u plasmid origin of replication"
     promoter        5533..5934
                     /label="ADH1 promoter"
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             5962..6402
                     /label="GAL4 DNA binding domain"
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
ORIGIN
        1 ccgggcatgt ctgcgggaga cccccgcgtg ggatccgggt ccctggactc cttcatgttc
       61 tccataccct tggtcgcgct taacgtggga gtgaggcgcc gcctatcgct gttcttgaac
      121 cctcggacgc ccgtggcggc cgactggacc ttgctcccgg aggagatggg cttcgagtac
      181 ttggagatcc gagagctgga aacgcgccct gaccccactc gcagtttgtt ggatgcctgg
      241 caggggcgct ctggcgcgtc tgtcggcagg ctgctagagc tgctggcctt gttagaccgt
      301 gaggatatac tgaaggagct gaagtcgcgc atcgaggagg actgccagaa atacttaggt
      361 aagcagcaga accaggagtc cgagaagcct ttacaggtgg ccagagtgga aagcagtgtc
      421 ccacaaacaa aggaactggg aggcatcacc acccttgatg accccctagg acaaacgccg
      481 gaacttttcg atgcctttat ctgctactgc cccaacgata tcgagtttgt gcaggagatg
      541 atccggcaac tagaacagac agactatcgg cttaagttgt gtgtgtccga ccgtgacgtc
      601 ctgccgggca cctgtgtctg gtccattgcc agcgagctaa ttgagaaaag gtgtcgccgc
      661 atggtggtgg ttgtttctga cgattatcta cagagcaagg aatgtgactt ccagaccaag
      721 tttgcactca gcctgtctcc aggtgtccaa cagaagcgac tgattcctat taaatacaag
      781 gcgatgaaga aggactttcc cagtatcctg cggttcatca ctatatgcga ctataccaac
      841 ccttgcacca agtcctggtt ctggacccgc cttgccaagg ctttgtccct gccctgactc
      901 gagctgcagc caagctaatt ccgggcgaat ttcttatgat ttatgatttt tattattaaa
      961 taagttataa aaaaaataag tgtatacaaa ttttaaagtg actcttaggt tttaaaacga
     1021 aaattcttgt tcttgagtaa ctctttcctg taggtcaggt tgctttctca ggtatagcat
     1081 gaggtcgctc ttattgacca cacctctacc ggcatgccgg caagtgcaca aacaatactt
     1141 aaataaatac tactcagtaa taacctattt cttagcattt ttgacgaaat ttgctatttt
     1201 gttagagtct tttacaccat ttgtctccac acctccgctt acatcaacac caataacgcc
     1261 atttaatcta agcgcatcac caacattttc tggcgtcagt ccaccagcta acataaaatg
     1321 taagctttcg gggctctctt gccttccaac ccagtcagaa atcgagttcc aatccaaaag
     1381 ttcacctgtc ccacctgctt ctgaatcaaa caagggaata aacgaatgag gtttctgtga
     1441 agctgcactg agtagtatgt tgcagtcttt tggaaatacg agtcttttaa taactggcaa
     1501 accgaggaac tcttggtatt cttgccacga ctcatctcca tgcagttgga cgatatcaat
     1561 gccgtaatca ttgaccagag ccaaaacatc ctccttaggt tgattacgaa acacgccaac
     1621 caagtatttc ggagtgcctg aactattttt atatgctttt acaagacttg aaattttcct
     1681 tgcaataacc gggtcaattg ttctctttct attgggcaca catataatac ccagcaagtc
     1741 agcatcggaa tctagagcac attctgcggc ctctgtgctc tgcaagccgc aaactttcac
     1801 caatggacca gaactacctg tgaaattaat aacagacata ctccaagctg cctttgtgtg
     1861 cttaatcacg tatactcacg tgctcaatag tcaccaatgc cctccctctt ggccctctcc
     1921 ttttcttttt tcgaccgaat taattcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     1981 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     2041 ggtgcctaat gagtgaggta actcacatta attgcgttgc gctcactgcc cgctttccag
     2101 tcgggaaacc tgtcgtgcca gctggattaa tgaatcggcc aacgcgcggg gagaggcggt
     2161 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     2221 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     2281 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     2341 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     2401 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     2461 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     2521 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     2581 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     2641 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     2701 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     2761 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     2821 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     2881 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     2941 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     3001 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     3061 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     3121 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     3181 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     3241 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     3301 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     3361 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     3421 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     3481 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     3541 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     3601 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     3661 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     3721 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     3781 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     3841 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     3901 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     3961 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     4021 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     4081 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     4141 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     4201 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     4261 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     4321 aactatgcgg catcagagca gattgtactg agagtgcacc ataacgcatt taagcataaa
     4381 cacgcactat gccgttcttc tcatgtatat atatatacag gcaacacgca gatataggtg
     4441 cgacgtgaac agtgagctgt atgtgcgcag ctcgcgttgc attttcggaa gcgctcgttt
     4501 tcggaaacgc tttgaagttc ctattccgaa gttcctattc tctagctaga aagtatagga
     4561 acttcagagc gcttttgaaa accaaaagcg ctctgaagac gcactttcaa aaaaccaaaa
     4621 acgcaccgga ctgtaacgag ctactaaaat attgcgaata ccgcttccac aaacattgct
     4681 caaaagtatc tctttgctat atatctctgt gctatatccc tatataacct acccatccac
     4741 ctttcgctcc ttgaacttgc atctaaactc gacctctaca ttttttatgt ttatctctag
     4801 tattactctt tagacaaaaa aattgtagta agaactattc atagagtgaa tcgaaaacaa
     4861 tacgaaaatg taaacatttc ctatacgtag tatatagaga caaaatagaa gaaaccgttc
     4921 ataattttct gaccaatgaa gaatcatcaa cgctatcact ttctgttcac aaagtatgcg
     4981 caatccacat cggtatagaa tataatcggg gatgccttta tcttgaaaaa atgcacccgc
     5041 agcttcgcta gtaatcagta aacgcgggaa gtggagtcag gcttttttta tggaagagaa
     5101 aatagacacc aaagtagcct tcttctaacc ttaacggacc tacagtgcaa aaagttatca
     5161 agagactgca ttatagagcg cacaaaggag aaaaaaagta atctaagatg ctttgttaga
     5221 aaaatagcgc tctcgggatg catttttgta gaacaaaaaa gaagtataga ttctttgttg
     5281 gtaaaatagc gctctcgcgt tgcatttctg ttctgtaaaa atgcagctca gattctttgt
     5341 ttgaaaaatt agcgctctcg cgttgcattt ttgttttaca aaaatgaagc acagattctt
     5401 cgttggtaaa atagcgcttt cgcgttgcat ttctgttctg taaaaatgca gctcagattc
     5461 tttgtttgaa aaattagcgc tctcgcgttg catttttgtt ctacaaaatg aagcacagat
     5521 gcttcgttgc ttgcatgcaa cttcttttct ttttttttct tttctctctc ccccgttgtt
     5581 gtctcaccat atccgcaatg acaaaaaaaa tgatggaaga cactaaagga aaaaattaac
     5641 gacaaagaca gcaccaacag atgtcgttgt tccagagctg atgaggggta tcttcgaaca
     5701 cacgaaactt tttccttcct tcattcacgc acactactct ctaatgagca acggtatacg
     5761 gccttccttc cagttacttg aatttgaaat aaaaaaagtt tgccgctttg ctatcaagta
     5821 taaatagacc tgcaattatt aatcttttgt ttcctcgtca ttgttctcgt tccctttctt
     5881 ccttgtttct ttttctgcac aatatttcaa gctataccaa gcatacaatc aactccaagc
     5941 ttgaagcaag cctcctgaaa gatgaagcta ctgtcttcta tcgaacaagc atgcgatatt
     6001 tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga agtgcgccaa gtgtctgaag
     6061 aacaactggg agtgtcgcta ctctcccaaa accaaaaggt ctccgctgac tagggcacat
     6121 ctgacagaag tggaatcaag gctagaaaga ctggaacagc tatttctact gatttttcct
     6181 cgagaagacc ttgacatgat tttgaaaatg gattctttac aggatataaa agcattgtta
     6241 acaggattat ttgtacaaga taatgtgaat aaagatgccg tcacagatag attggcttca
     6301 gtggagactg atatgcctct aacattgaga cagcatagaa taagtgcgac atcatcatcg
     6361 gaagagagta gtaacaaagg tcaaagacag ttgactgtat cgccggaatt c
//
