LOCUS       40924_21185        8539 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast expression vector pGBKT7-Cezanne(444-858), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8539)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8539)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 8539)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8539)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8539
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        30..734
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             762..1202
                     /label=GAL4 DNA binding domain
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     promoter        1213..1231
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1251..1280
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             1305..2549
                     /codon_start=1
                     /note="unnamed protein product; Cezanne(445-858)"
                     /protein_id="SJL86983.1"
                     /translation="NVKWIPLSSDAQAPLAQPESPTASAGDEPRSTPESGDSDKESVGS
                     SSTSNEGGRRKEKSKRDREKDKKRADSVANKLGSFGKTLGSKLKKNMGGLMHSKGSKPG
                     GVGTGLGGSSGTETLEKKKKNSLKSWKGGKEEAAGDGPVSEKPPAESVGNGGSKYSQEV
                     MQSLSILRTAMQGEGKFIFVGTLKMGHRHQYQEEMIQRYLSDAEERFLAEQKQKEAERK
                     IMNGGIGGGPPPAKKPEPDAREEQPTGPPAESRAMAFSTGYPGDFTIPRPSGGGVHCQE
                     PRRQLAGGPCVGGLPPYATFPRQCPPGRPYPHQDSIPSLEPGSHSKDGLHRGALLPPPY
                     RVADSYSNGYREPPEPDGWAGGLRGLPPTQTKCKQPNCSFYGHPETNNFCSCCYREELR
                     RREREPDGELLVHRF"
     terminator      2573..2620
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      2647..2834
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2858..2874)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2882..2898)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2906..2936)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2951..2972)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3260..3848)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    complement(4177..4224)
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(4460..5251)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(5252..5356)
                     /label=AmpR promoter
     rep_origin      complement(5383..6725)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        6984..7265
                     /label=TRP1 promoter
     CDS             7266..7937
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
     rep_origin      complement(8043..8498)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 cggtgcgggc ctcttcgcta ttacgccaga tccttttgtt gtttccgggt gtacaatatg
       61 gacttcctct tttctggcaa ccaaacccat acatcgggat tcctataata ccttcgttgg
      121 tctccctaac atgtaggtgg cggaggggag atatacaata gaacagatac cagacaagac
      181 ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg tacataacga
      241 actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca ctaccctttt
      301 tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct ttttttttct
      361 tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaaa tgatggaaga
      421 cactaaagga aaaaattaac gacaaagaca gcaccaacag atgtcgttgt tccagagctg
      481 atgaggggta tctcgaagca cacgaaactt tttccttcct tcattcacgc acactactct
      541 ctaatgagca acggtatacg gccttccttc cagttacttg aatttgaaat aaaaaaagtt
      601 tgctgtcttg ctatcaagta taaatagacc tgcaattatt aatcttttgt ttcctcgtca
      661 ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa gctataccaa
      721 gcatacaatc aactccaagc ttgaagcaag cctcctgaaa gatgaagcta ctgtcttcta
      781 tcgaacaagc atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga
      841 agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt
      901 ctccgctgac tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc
      961 tatttctact gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac
     1021 aggatataaa agcattgtta acaggattat ttgtacaaga taatgtgaat aaagatgccg
     1081 tcacagatag attggcttca gtggagactg atatgcctct aacattgaga cagcatagaa
     1141 taagtgcgac atcatcatcg gaagagagta gtaacaaagg tcaaagacag ttgactgtat
     1201 cgccggaatt tgtaatacga ctcactatag ggcgagccgc catcatggag gagcagaagc
     1261 tgatctcaga ggaggacctg catatggcca tggaggccga attcaatgtg aagtggatcc
     1321 cactgtcctc tgatgcacag gctcctctgg cccagcctga gtcccccacc gcctcagctg
     1381 gagatgagcc ccggtccact cctgagtctg gagactcaga caaggagtca gttggcagca
     1441 gttccaccag caacgagggc ggccggcgga aggagaagtc aaagcgagat cgggagaagg
     1501 acaagaagag agcagattct gtggctaaca aactgggcag ctttggcaaa accttgggca
     1561 gcaagctcaa gaagaacatg gggggcctga tgcacagcaa gggttcaaag cctggagggg
     1621 tggggacagg gttgggagga agcagcggca ctgagacact ggagaagaag aagaaaaact
     1681 cactgaagag ctggaagggt ggcaaggagg aggcagctgg ggatgggcct gtgtctgaga
     1741 agcccccagc tgagtctgtt ggtaacggag ggagcaagta tagccaggag gtgatgcaga
     1801 gcctgagcat tctgaggact gccatgcaag gggaggggaa gtttattttt gttggaaccc
     1861 tgaagatggg tcaccgtcac cagtatcagg aggaaatgat ccagcgctac ctttctgatg
     1921 ctgaggagag attcctggca gaacagaagc agaaggaggc agagaggaag atcatgaatg
     1981 gaggaatagg gggtggccct cctccagcca aaaagccaga gccagatgct agggaagagc
     2041 agccgaccgg tcccccagca gagtccaggg caatggcatt ttccactggc taccctgggg
     2101 actttactat ccctcggccg tctgggggcg gagtccactg ccaggaaccc cggaggcagt
     2161 tggcaggggg tccatgtgtc gggggcctac caccatatgc caccttcccc agacagtgcc
     2221 ctcctgggcg accctacccc caccaggaca gcatcccttc tctggagcca ggcagccact
     2281 ctaaggatgg acttcacagg ggtgccttgt taccaccccc ctaccgagtg gctgattcct
     2341 atagcaatgg ctacagagag ccccctgagc cagatggatg ggctggaggt ctccggggcc
     2401 ttcccccaac tcagaccaaa tgcaaacaac cgaactgcag cttctatgga caccctgaga
     2461 caaacaactt ctgttcctgt tgttacaggg aagaactgag gaggagggag cgggaaccgg
     2521 atggggagct cctggtgcac aggttctgag tcgacctgca gcggccgcat aactagcata
     2581 accccttggg gcctctaaac gggtcttgag gggttttttg cgcgcttgca gccaagctaa
     2641 ttccgggcga atttcttatg atttatgatt tttattatta aataagttat aaaaaaaata
     2701 agtgtataca aattttaaag tgactcttag gttttaaaac gaaaattctt attcttgagt
     2761 aactctttcc tgtaggtcag gttgctttct caggtatagc atgaggtcgc tcttattgac
     2821 cacacctcta ccggcatgca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     2881 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     2941 ggggtgccta atgagtgagg taactcacat taattgcgtt gcgctcactg cccgctttcc
     3001 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     3061 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     3121 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     3181 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     3241 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     3301 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     3361 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     3421 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     3481 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     3541 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     3601 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     3661 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     3721 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     3781 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     3841 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     3901 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     3961 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaacctgag gctatggcag
     4021 ggcctgccgc cccgacgttg gctgcgagcc ctgggccttc acccgaactt ggggggtggg
     4081 gtggggaaaa ggaagaaacg cgggcgtatt ggccccaatg gggtctcggt ggggtatcga
     4141 cagagtgcca gccctgggac cgaaccccgc gtttatgaac aaacgaccca acaccgtgcg
     4201 ttttattctg tctttttatt gccgtcatag cgcgggttcc ttccggtatt gtctccttcc
     4261 gtgtttcagt tagcctcccc ctagggtggg cgaagaactc cagcatgaga tccccgcgct
     4321 ggaggatcat ccagccggcg tcccggaaaa cgattccgaa gcccaacctt tcatagaagg
     4381 cggcggtgga atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg gttcatttcg
     4441 aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg
     4501 aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct
     4561 cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc
     4621 ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg
     4681 catcgtcatg ggtcacgacg agatcctcgc cgtcgggcat gctcgccttg agcctggcga
     4741 acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac
     4801 cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc
     4861 aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct
     4921 cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc
     4981 agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg
     5041 ccagccacga tagccgcgct gcctcgtctt gcagttcatt cagggcaccg gacaggtcgg
     5101 tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc
     5161 agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag
     5221 aacctgcgtg caatccatct tgttcaatca tactcttcct ttttcaatat tattgaagca
     5281 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     5341 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgaacgaagc atctgtgctt
     5401 cattttgtag aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc
     5461 tgcattttta cagaacagaa atgcaacgcg aaagcgctat tttaccaacg aagaatctgt
     5521 gcttcatttt tgtaaaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc
     5581 tgagctgcat ttttacagaa cagaaatgca acgcgagagc gctattttac caacaaagaa
     5641 tctatacttc ttttttgttc tacaaaaatg catcccgaga gcgctatttt tctaacaaag
     5701 catcttagat tacttttttt ctcctttgtg cgctctataa tgcagtctct tgataacttt
     5761 ttgcactgta ggtccgttaa ggttagaaga aggctacttt ggtgtctatt ttctcttcca
     5821 taaaaaaagc ctgactccac ttcccgcgtt tactgattac tagcgaagct gcgggtgcat
     5881 tttttcaaga taaaggcatc cccgattata ttctataccg atgtggattg cgcatacttt
     5941 gtgaacagaa agtgatagcg ttgatgattc ttcattggtc agaaaattat gaacggtttc
     6001 ttctattttg tctctatata ctacgtatag gaaatgttta cattttcgta ttgttttcga
     6061 ttcactctat gaatagttct tactacaatt tttttgtcta aagagtaata ctagagataa
     6121 acataaaaaa tgtagaggtc gagtttagat gcaagttcaa ggagcgaaag gtggatgggt
     6181 aggttatata gggatatagc acagagatat atagcaaaga gatacttttg agcaatgttt
     6241 gtggaagcgg tattcgcaat attttagtag ctcgttacag tccggtgcgt ttttggtttt
     6301 ttgaaagtgc gtcttcagag cgcttttggt tttcaaaagc gctctgaagt tcctatactt
     6361 tctagagaat aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac gagcgcttcc
     6421 gaaaatgcaa cgcgagctgc gcacatacag ctcactgttc acgtcgcacc tatatctgcg
     6481 tgttgcctgt atatatatat acatgagaag aacggcatag tgcgtgttta tgcttaaatg
     6541 cgtacttata tgcgtctatt tatgtaggat gaaaggtagt ctagtacctc ctgtgatatt
     6601 atcccattcc atgcggggta tcgtatgctt ccttcagcac taccctttag ctgttctata
     6661 tgctgccact cctcaattgg attagtctca tccttcaatg ctatcatttc ctttgatatt
     6721 ggatcatatt aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     6781 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     6841 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     6901 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     6961 gtactgagag tgcaccatag atcaacgaca ttactatata tataatatag gaagcattta
     7021 atagaacagc atcgtaatat atgtgtactt tgcagttatg acgccagatg gcagtagtgg
     7081 aagatattct ttattgaaaa atagcttgtc accttacgta caatcttgat ccggagcttt
     7141 tctttttttg ccgattaaga attaattcgg tcgaaaaaag aaaaggagag ggccaagagg
     7201 gagggcattg gtgactattg agcacgtgag tatacgtgat taagcacaca aaggcagctt
     7261 ggagtatgtc tgttattaat ttcacaggta gttctggtcc attggtgaaa gtttgcggct
     7321 tgcagagcac agaggccgca gaatgtgctc tagattccga tgctgacttg ctgggtatta
     7381 tatgtgtgcc caatagaaag agaacaattg acccggttat tgcaaggaaa atttcaagtc
     7441 ttgtaaaagc atataaaaat agttcaggca ctccgaaata cttggttggc gtgtttcgta
     7501 atcaacctaa ggaggatgtt ttggctctgg tcaatgatta cggcattgat atcgtccaac
     7561 tgcatggaga tgagtcgtgg caagaatacc aagagttcct cggtttgcca gttattaaaa
     7621 gactcgtatt tccaaaagac tgcaacatac tactcagtgc agcttcacag aaacctcatt
     7681 cgtttattcc cttgtttgat tcagaagcag gtgggacagg tgaacttttg gattggaact
     7741 cgatttctga ctgggttgga aggcaagaga gccccgaaag cttacatttt atgttagctg
     7801 gtggactgac gccagaaaat gttggtgatg cgcttagatt aaatggcgtt attggtgttg
     7861 atgtaagcgg aggtgtggag acaaatggtg taaaagactc taacaaaata gcaaatttcg
     7921 tcaaaaatgc taagaaatag gttattactg agtagtattt atttaagtat tgtttgtgca
     7981 cttgccgatc tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     8041 ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     8101 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     8161 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     8221 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     8281 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     8341 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     8401 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     8461 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt caggctgcgc
     8521 aactgttggg aagggcgat
//
