LOCUS       V006085                 9823 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006085
VERSION     V006085
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9823)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9823)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9823)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9823)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9823
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        30..734
                     /label="ADH1 promoter"
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             762..1202
                     /label="GAL4 DNA binding domain"
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     promoter        1213..1231
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1251..1280
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             1305..3830
                     /gene="OTUD7B"
                     /label="OTU domain-containing protein 7B"
                     /note="OTU domain-containing protein 7B from Homo sapiens.
                     Accession#: Q6GQQ9"
     terminator      3857..3904
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     terminator      3931..4118
                     /label="ADH1 terminator"
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4142..4158)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4166..4182)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4190..4220)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4235..4256)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4544..5132)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     polyA_signal    complement(5461..5508)
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(5744..6535)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(6536..6640)
                     /label="AmpR promoter"
     rep_origin      complement(6667..8009)
                     /direction=LEFT
                     /label="2u ori"
                     /note="yeast 2u plasmid origin of replication"
     promoter        8268..8549
                     /label="TRP1 promoter"
     CDS             8550..9221
                     /label="TRP1"
                     /note="phosphoribosylanthranilate isomerase, required for
                     tryptophan biosynthesis"
     rep_origin      complement(9327..9782)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 cggtgcgggc ctcttcgcta ttacgccaga tccttttgtt gtttccgggt gtacaatatg
       61 gacttcctct tttctggcaa ccaaacccat acatcgggat tcctataata ccttcgttgg
      121 tctccctaac atgtaggtgg cggaggggag atatacaata gaacagatac cagacaagac
      181 ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg tacataacga
      241 actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca ctaccctttt
      301 tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct ttttttttct
      361 tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaaa tgatggaaga
      421 cactaaagga aaaaattaac gacaaagaca gcaccaacag atgtcgttgt tccagagctg
      481 atgaggggta tctcgaagca cacgaaactt tttccttcct tcattcacgc acactactct
      541 ctaatgagca acggtatacg gccttccttc cagttacttg aatttgaaat aaaaaaagtt
      601 tgctgtcttg ctatcaagta taaatagacc tgcaattatt aatcttttgt ttcctcgtca
      661 ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa gctataccaa
      721 gcatacaatc aactccaagc ttgaagcaag cctcctgaaa gatgaagcta ctgtcttcta
      781 tcgaacaagc atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga
      841 agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt
      901 ctccgctgac tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc
      961 tatttctact gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac
     1021 aggatataaa agcattgtta acaggattat ttgtacaaga taatgtgaat aaagatgccg
     1081 tcacagatag attggcttca gtggagactg atatgcctct aacattgaga cagcatagaa
     1141 taagtgcgac atcatcatcg gaagagagta gtaacaaagg tcaaagacag ttgactgtat
     1201 cgccggaatt tgtaatacga ctcactatag ggcgagccgc catcatggag gagcagaagc
     1261 tgatctcaga ggaggacctg catatggcca tggaggccga attcaccctg gacatggatg
     1321 ctgttctgtc agattttgtc cgttccacag gagcagagcc agggctagcg cgagatctcc
     1381 tagaaggaaa gaattgggat gtgaatgccg ccctcagtga ttttgaacag ctacgtcaag
     1441 tccatgctgg aaacctaccc ccatccttta gtgaggggag tggtggctcc aggacccctg
     1501 aaaaagggtt ttctgacaga gagcctactc gccctccccg acccatcctc cagcggcagg
     1561 atgacatcgt tcaagaaaaa cgcctgtcta ggggcatctc ccacgccagc tccagcattg
     1621 tttccctggc ccggtcccat gtctcctcca atggtggggg tggggggagc aatgagcacc
     1681 ccctggaaat gcccatctgt gccttccagc ttccagatct cactgtatac aatgaagact
     1741 tccgcagctt catagagaga gacctcattg agcagtccat gctggttgcc ttggaacagg
     1801 cagggcgttt gaactggtgg gtgagtgtgg atcccacctc tcagaggctg cttcctttgg
     1861 caactactgg agatgggaac tgcctcctgc atgcagcctc ccttggaatg tggggtttcc
     1921 atgatcggga cttgatgctg cggaaagctt tgtatgcact gatggagaag ggagttgaga
     1981 aggaagcgtt gaaaaggcgc tggaggtggc agcagacaca gcagaataaa gagtcagggc
     2041 tggtatacac agaagatgaa tggcagaagg agtggaatga actgatcaag cttgcctcaa
     2101 gtgaaccccg aatgcatcta ggtaccaatg gagccaactg tggtggggtg gagagttctg
     2161 aggagcctgt atatgagagc cttgaagagt ttcacgtctt tgtccttgct catgtgctta
     2221 ggaggcccat agtcgtcgtg gcagacacca tgctgaggga ctccggaggg gaagcatttg
     2281 cccctattcc ctttggagga atctatctgc ctttggaggt cccagccagc cagtgtcacc
     2341 gctcccctct ggtgctcgcc tatgatcagg cccacttttc tgcactcgtg tccatggagc
     2401 agaaggagaa taccaaggaa caagctgtga tcccacttac agattcagag tataagctgc
     2461 tgcccttgca ctttgctgtg gaccctggaa agggctggga gtggggcaaa gatgatagtg
     2521 acaatgtccg attggccagt gtaattctgt ccctagaggt caaattgcat ctgctgcata
     2581 gctacatgaa tgtgaagtgg atcccactgt cctctgatgc acaggctcct ctggcccagc
     2641 ctgagtcccc caccgcctca gctggagatg agccccggtc cactcctgag tctggagact
     2701 cagacaagga gtcagttggc agcagttcca ccagcaacga gggcggccgg cggaaggaga
     2761 agtcaaagcg agatcgggag aaggacaaga agagagcaga ttctgtggct aacaaactgg
     2821 gcagctttgg caaaaccttg ggcagcaagc tcaagaagaa catggggggc ctgatgcaca
     2881 gcaagggttc aaagcctgga ggggtgggga cagggttggg aggaagcagc ggcactgaga
     2941 cactggagaa gaagaagaaa aactcactga agagctggaa gggtggcaag gaggaggcag
     3001 ctggggatgg gcctgtgtct gagaagcccc cagctgagtc tgttggtaac ggagggagca
     3061 agtatagcca ggaggtgatg cagagcctga gcattctgag gactgccatg caaggggagg
     3121 ggaagtttat ttttgttgga accctgaaga tgggtcaccg tcaccagtat caggaggaaa
     3181 tgatccagcg ctacctttct gatgctgagg agagattcct ggcagaacag aagcagaagg
     3241 aggcagagag gaagatcatg aatggaggaa tagggggtgg ccctcctcca gccaaaaagc
     3301 cagagccaga tgctagggaa gagcagccga ccggtccccc agcagagtcc agggcaatgg
     3361 cattttccac tggctaccct ggggacttta ctatccctcg gccgtctggg ggcggagtcc
     3421 actgccagga accccggagg cagttggcag ggggtccatg tgtcgggggc ctaccaccat
     3481 atgccacctt ccccagacag tgccctcctg ggcgacccta cccccaccag gacagcatcc
     3541 cttctctgga gccaggcagc cactctaagg atggacttca caggggtgcc ttgttaccac
     3601 ccccctaccg agtggctgat tcctatagca atggctacag agagccccct gagccagatg
     3661 gatgggctgg aggtctccgg ggccttcccc caactcagac caaatgcaaa caaccgaact
     3721 gcagcttcta tggacaccct gagacaaaca acttctgttc ctgttgttac agggaagaac
     3781 tgaggaggag ggagcgggaa ccggatgggg agctcctggt gcacaggttc tgagtcgacc
     3841 tgcagcggcc gcataactag cataacccct tggggcctct aaacgggtct tgaggggttt
     3901 tttgcgcgct tgcagccaag ctaattccgg gcgaatttct tatgatttat gatttttatt
     3961 attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta
     4021 aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta
     4081 tagcatgagg tcgctcttat tgaccacacc tctaccggca tgcaagcttg gcgtaatcat
     4141 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     4201 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gaggtaactc acattaattg
     4261 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     4321 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     4381 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     4441 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     4501 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     4561 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     4621 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     4681 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     4741 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     4801 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     4861 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     4921 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     4981 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     5041 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     5101 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     5161 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     5221 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     5281 atgagtaacc tgaggctatg gcagggcctg ccgccccgac gttggctgcg agccctgggc
     5341 cttcacccga acttgggggg tggggtgggg aaaaggaaga aacgcgggcg tattggcccc
     5401 aatggggtct cggtggggta tcgacagagt gccagccctg ggaccgaacc ccgcgtttat
     5461 gaacaaacga cccaacaccg tgcgttttat tctgtctttt tattgccgtc atagcgcggg
     5521 ttccttccgg tattgtctcc ttccgtgttt cagttagcct ccccctaggg tgggcgaaga
     5581 actccagcat gagatccccg cgctggagga tcatccagcc ggcgtcccgg aaaacgattc
     5641 cgaagcccaa cctttcatag aaggcggcgg tggaatcgaa atctcgtgat ggcaggttgg
     5701 gcgtcgcttg gtcggttcat ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa
     5761 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc
     5821 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct
     5881 gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt
     5941 ccaccatgat attcggcaag caggcatcgt catgggtcac gacgagatcc tcgccgtcgg
     6001 gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt
     6061 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat
     6121 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg
     6181 catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc
     6241 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag
     6301 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcttgcagtt
     6361 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca
     6421 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata
     6481 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatactct
     6541 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     6601 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     6661 cacctgaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc gagagcgcta
     6721 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgaaagcg
     6781 ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc aacgcgagag
     6841 cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga
     6901 gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa aatgcatccc
     6961 gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt tgtgcgctct
     7021 ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag aagaaggcta
     7081 ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg cgtttactga
     7141 ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat tatattctat
     7201 accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg attcttcatt
     7261 ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt ataggaaatg
     7321 tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac aatttttttg
     7381 tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt agatgcaagt
     7441 tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag atatatagca
     7501 aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta gtagctcgtt
     7561 acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt tggttttcaa
     7621 aagcgctctg aagttcctat actttctaga gaataggaac ttcggaatag gaacttcaaa
     7681 gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat acagctcact
     7741 gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat atatacatga gaagaacggc
     7801 atagtgcgtg tttatgctta aatgcgtact tatatgcgtc tatttatgta ggatgaaagg
     7861 tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat gcttccttca
     7921 gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt ctcatccttc
     7981 aatgctatca tttcctttga tattggatca tattaagaaa ccattattat catgacatta
     8041 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     8101 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     8161 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     8221 aactatgcgg catcagagca gattgtactg agagtgcacc atagatcaac gacattacta
     8281 tatatataat ataggaagca tttaatagaa cagcatcgta atatatgtgt actttgcagt
     8341 tatgacgcca gatggcagta gtggaagata ttctttattg aaaaatagct tgtcacctta
     8401 cgtacaatct tgatccggag cttttctttt tttgccgatt aagaattaat tcggtcgaaa
     8461 aaagaaaagg agagggccaa gagggagggc attggtgact attgagcacg tgagtatacg
     8521 tgattaagca cacaaaggca gcttggagta tgtctgttat taatttcaca ggtagttctg
     8581 gtccattggt gaaagtttgc ggcttgcaga gcacagaggc cgcagaatgt gctctagatt
     8641 ccgatgctga cttgctgggt attatatgtg tgcccaatag aaagagaaca attgacccgg
     8701 ttattgcaag gaaaatttca agtcttgtaa aagcatataa aaatagttca ggcactccga
     8761 aatacttggt tggcgtgttt cgtaatcaac ctaaggagga tgttttggct ctggtcaatg
     8821 attacggcat tgatatcgtc caactgcatg gagatgagtc gtggcaagaa taccaagagt
     8881 tcctcggttt gccagttatt aaaagactcg tatttccaaa agactgcaac atactactca
     8941 gtgcagcttc acagaaacct cattcgttta ttcccttgtt tgattcagaa gcaggtggga
     9001 caggtgaact tttggattgg aactcgattt ctgactgggt tggaaggcaa gagagccccg
     9061 aaagcttaca ttttatgtta gctggtggac tgacgccaga aaatgttggt gatgcgctta
     9121 gattaaatgg cgttattggt gttgatgtaa gcggaggtgt ggagacaaat ggtgtaaaag
     9181 actctaacaa aatagcaaat ttcgtcaaaa atgctaagaa ataggttatt actgagtagt
     9241 atttatttaa gtattgtttg tgcacttgcc gatctatgcg gtgtgaaata ccgcacagat
     9301 gcgtaaggag aaaataccgc atcaggaaat tgtaaacgtt aatattttgt taaaattcgc
     9361 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     9421 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     9481 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     9541 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     9601 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     9661 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     9721 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     9781 gtccattcgc cattcaggct gcgcaactgt tgggaagggc gat
//
