LOCUS       40924_21175        8578 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast expression vector pGBKT7-Cezanne(17-443), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8578)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8578)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 8578)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8578)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8578
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        30..734
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             762..1202
                     /label=GAL4 DNA binding domain
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     promoter        1213..1231
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1251..1280
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             1305..2588
                     /codon_start=1
                     /note="unnamed protein product; Cezanne(17-444)"
                     /protein_id="SJL86979.1"
                     /translation="TLDMDAVLSDFVRSTGAEPGLARDLLEGKNWDVNAALSDFEQLRQ
                     VHAGNLPPSFSEGSGGSRTPEKGFSDREPTRPPRPILQRQDDIVQEKRLSRGISHASSS
                     IVSLARSHVSSNGGGGGSNEHPLEMPICAFQLPDLTVYNEDFRSFIERDLIEQSMLVAL
                     EQAGRLNWWVSVDPTSQRLLPLATTGDGNCLLHAASLGMWGFHDRDLMLRKALYALMEK
                     GVEKEALKRRWRWQQTQQNKESGLVYTEDEWQKEWNELIKLASSEPRMHLGTNGANCGG
                     VESSEEPVYESLEEFHVFVLAHVLRRPIVVVADTMLRDSGGEAFAPIPFGGIYLPLEVP
                     ASQCHRSPLVLAYDQAHFSALVSMEQKENTKEQAVIPLTDSEYKLLPLHFAVDPGKGWE
                     WGKDDSDNVRLASVILSLEVKLHLLHSYM"
     terminator      2612..2659
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      2686..2873
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2897..2913)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2921..2937)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2945..2975)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2990..3011)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3299..3887)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    complement(4216..4263)
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(4499..5290)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(5291..5395)
                     /label=AmpR promoter
     rep_origin      complement(5422..6764)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        7023..7304
                     /label=TRP1 promoter
     CDS             7305..7976
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
     rep_origin      complement(8082..8537)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 cggtgcgggc ctcttcgcta ttacgccaga tccttttgtt gtttccgggt gtacaatatg
       61 gacttcctct tttctggcaa ccaaacccat acatcgggat tcctataata ccttcgttgg
      121 tctccctaac atgtaggtgg cggaggggag atatacaata gaacagatac cagacaagac
      181 ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg tacataacga
      241 actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca ctaccctttt
      301 tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct ttttttttct
      361 tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaaa tgatggaaga
      421 cactaaagga aaaaattaac gacaaagaca gcaccaacag atgtcgttgt tccagagctg
      481 atgaggggta tctcgaagca cacgaaactt tttccttcct tcattcacgc acactactct
      541 ctaatgagca acggtatacg gccttccttc cagttacttg aatttgaaat aaaaaaagtt
      601 tgctgtcttg ctatcaagta taaatagacc tgcaattatt aatcttttgt ttcctcgtca
      661 ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa gctataccaa
      721 gcatacaatc aactccaagc ttgaagcaag cctcctgaaa gatgaagcta ctgtcttcta
      781 tcgaacaagc atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga
      841 agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt
      901 ctccgctgac tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc
      961 tatttctact gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac
     1021 aggatataaa agcattgtta acaggattat ttgtacaaga taatgtgaat aaagatgccg
     1081 tcacagatag attggcttca gtggagactg atatgcctct aacattgaga cagcatagaa
     1141 taagtgcgac atcatcatcg gaagagagta gtaacaaagg tcaaagacag ttgactgtat
     1201 cgccggaatt tgtaatacga ctcactatag ggcgagccgc catcatggag gagcagaagc
     1261 tgatctcaga ggaggacctg catatggcca tggaggccga attcaccctg gacatggatg
     1321 ctgttctgtc agattttgtc cgttccacag gagcagagcc agggctagcg cgagatctcc
     1381 tagaaggaaa gaattgggat gtgaatgccg ccctcagtga ttttgaacag ctacgtcaag
     1441 tccatgctgg aaacctaccc ccatccttta gtgaggggag tggtggctcc aggacccctg
     1501 aaaaagggtt ttctgacaga gagcctactc gccctccccg acccatcctc cagcggcagg
     1561 atgacatcgt tcaagaaaaa cgcctgtcta ggggcatctc ccacgccagc tccagcattg
     1621 tttccctggc ccggtcccat gtctcctcca atggtggggg tggggggagc aatgagcacc
     1681 ccctggaaat gcccatctgt gccttccagc ttccagatct cactgtatac aatgaagact
     1741 tccgcagctt catagagaga gacctcattg agcagtccat gctggttgcc ttggaacagg
     1801 cagggcgttt gaactggtgg gtgagtgtgg atcccacctc tcagaggctg cttcctttgg
     1861 caactactgg agatgggaac tgcctcctgc atgcagcctc ccttggaatg tggggtttcc
     1921 atgatcggga cttgatgctg cggaaagctt tgtatgcact gatggagaag ggagttgaga
     1981 aggaagcgtt gaaaaggcgc tggaggtggc agcagacaca gcagaataaa gagtcagggc
     2041 tggtatacac agaagatgaa tggcagaagg agtggaatga actgatcaag cttgcctcaa
     2101 gtgaaccccg aatgcatcta ggtaccaatg gagccaactg tggtggggtg gagagttctg
     2161 aggagcctgt atatgagagc cttgaagagt ttcacgtctt tgtccttgct catgtgctta
     2221 ggaggcccat agtcgtcgtg gcagacacca tgctgaggga ctccggaggg gaagcatttg
     2281 cccctattcc ctttggagga atctatctgc ctttggaggt cccagccagc cagtgtcacc
     2341 gctcccctct ggtgctcgcc tatgatcagg cccacttttc tgcactcgtg tccatggagc
     2401 agaaggagaa taccaaggaa caagctgtga tcccacttac agattcagag tataagctgc
     2461 tgcccttgca ctttgctgtg gaccctggaa agggctggga gtggggcaaa gatgatagtg
     2521 acaatgtccg attggccagt gtaattctgt ccctagaggt caaattgcat ctgctgcata
     2581 gctacatggt cgacctgcag cggccgcata actagcataa ccccttgggg cctctaaacg
     2641 ggtcttgagg ggttttttgc gcgcttgcag ccaagctaat tccgggcgaa tttcttatga
     2701 tttatgattt ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt
     2761 gactcttagg ttttaaaacg aaaattctta ttcttgagta actctttcct gtaggtcagg
     2821 ttgctttctc aggtatagca tgaggtcgct cttattgacc acacctctac cggcatgcaa
     2881 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     2941 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgaggt
     3001 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     3061 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     3121 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     3181 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     3241 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     3301 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     3361 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     3421 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     3481 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     3541 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     3601 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     3661 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     3721 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     3781 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     3841 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     3901 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     3961 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     4021 caatctaaag tatatatgag taacctgagg ctatggcagg gcctgccgcc ccgacgttgg
     4081 ctgcgagccc tgggccttca cccgaacttg gggggtgggg tggggaaaag gaagaaacgc
     4141 gggcgtattg gccccaatgg ggtctcggtg gggtatcgac agagtgccag ccctgggacc
     4201 gaaccccgcg tttatgaaca aacgacccaa caccgtgcgt tttattctgt ctttttattg
     4261 ccgtcatagc gcgggttcct tccggtattg tctccttccg tgtttcagtt agcctccccc
     4321 tagggtgggc gaagaactcc agcatgagat ccccgcgctg gaggatcatc cagccggcgt
     4381 cccggaaaac gattccgaag cccaaccttt catagaaggc ggcggtggaa tcgaaatctc
     4441 gtgatggcag gttgggcgtc gcttggtcgg ttcatttcga accccagagt cccgctcaga
     4501 agaactcgtc aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt
     4561 aaagcacgag gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag
     4621 ccaacgctat gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag
     4681 aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgtcatgg gtcacgacga
     4741 gatcctcgcc gtcgggcatg ctcgccttga gcctggcgaa cagttcggct ggcgcgagcc
     4801 cctgatgctc ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg
     4861 ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat
     4921 gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg
     4981 acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga
     5041 caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg
     5101 cctcgtcttg cagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc
     5161 gcccctgcgc tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc
     5221 agtcatagcc gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt
     5281 gttcaatcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     5341 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     5401 ttccccgaaa agtgccacct gaacgaagca tctgtgcttc attttgtaga acaaaaatgc
     5461 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa
     5521 tgcaacgcga aagcgctatt ttaccaacga agaatctgtg cttcattttt gtaaaacaaa
     5581 aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac
     5641 agaaatgcaa cgcgagagcg ctattttacc aacaaagaat ctatacttct tttttgttct
     5701 acaaaaatgc atcccgagag cgctattttt ctaacaaagc atcttagatt actttttttc
     5761 tcctttgtgc gctctataat gcagtctctt gataactttt tgcactgtag gtccgttaag
     5821 gttagaagaa ggctactttg gtgtctattt tctcttccat aaaaaaagcc tgactccact
     5881 tcccgcgttt actgattact agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc
     5941 ccgattatat tctataccga tgtggattgc gcatactttg tgaacagaaa gtgatagcgt
     6001 tgatgattct tcattggtca gaaaattatg aacggtttct tctattttgt ctctatatac
     6061 tacgtatagg aaatgtttac attttcgtat tgttttcgat tcactctatg aatagttctt
     6121 actacaattt ttttgtctaa agagtaatac tagagataaa cataaaaaat gtagaggtcg
     6181 agtttagatg caagttcaag gagcgaaagg tggatgggta ggttatatag ggatatagca
     6241 cagagatata tagcaaagag atacttttga gcaatgtttg tggaagcggt attcgcaata
     6301 ttttagtagc tcgttacagt ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc
     6361 gcttttggtt ttcaaaagcg ctctgaagtt cctatacttt ctagagaata ggaacttcgg
     6421 aataggaact tcaaagcgtt tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg
     6481 cacatacagc tcactgttca cgtcgcacct atatctgcgt gttgcctgta tatatatata
     6541 catgagaaga acggcatagt gcgtgtttat gcttaaatgc gtacttatat gcgtctattt
     6601 atgtaggatg aaaggtagtc tagtacctcc tgtgatatta tcccattcca tgcggggtat
     6661 cgtatgcttc cttcagcact accctttagc tgttctatat gctgccactc ctcaattgga
     6721 ttagtctcat ccttcaatgc tatcatttcc tttgatattg gatcatatta agaaaccatt
     6781 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
     6841 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     6901 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     6961 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccataga
     7021 tcaacgacat tactatatat ataatatagg aagcatttaa tagaacagca tcgtaatata
     7081 tgtgtacttt gcagttatga cgccagatgg cagtagtgga agatattctt tattgaaaaa
     7141 tagcttgtca ccttacgtac aatcttgatc cggagctttt ctttttttgc cgattaagaa
     7201 ttaattcggt cgaaaaaaga aaaggagagg gccaagaggg agggcattgg tgactattga
     7261 gcacgtgagt atacgtgatt aagcacacaa aggcagcttg gagtatgtct gttattaatt
     7321 tcacaggtag ttctggtcca ttggtgaaag tttgcggctt gcagagcaca gaggccgcag
     7381 aatgtgctct agattccgat gctgacttgc tgggtattat atgtgtgccc aatagaaaga
     7441 gaacaattga cccggttatt gcaaggaaaa tttcaagtct tgtaaaagca tataaaaata
     7501 gttcaggcac tccgaaatac ttggttggcg tgtttcgtaa tcaacctaag gaggatgttt
     7561 tggctctggt caatgattac ggcattgata tcgtccaact gcatggagat gagtcgtggc
     7621 aagaatacca agagttcctc ggtttgccag ttattaaaag actcgtattt ccaaaagact
     7681 gcaacatact actcagtgca gcttcacaga aacctcattc gtttattccc ttgtttgatt
     7741 cagaagcagg tgggacaggt gaacttttgg attggaactc gatttctgac tgggttggaa
     7801 ggcaagagag ccccgaaagc ttacatttta tgttagctgg tggactgacg ccagaaaatg
     7861 ttggtgatgc gcttagatta aatggcgtta ttggtgttga tgtaagcgga ggtgtggaga
     7921 caaatggtgt aaaagactct aacaaaatag caaatttcgt caaaaatgct aagaaatagg
     7981 ttattactga gtagtattta tttaagtatt gtttgtgcac ttgccgatct atgcggtgtg
     8041 aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa acgttaatat
     8101 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     8161 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     8221 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     8281 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     8341 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     8401 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     8461 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     8521 gccgctacag ggcgcgtcca ttcgccattc aggctgcgca actgttggga agggcgat
//
