LOCUS       40924_21170        9011 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast two-hybrid vector pGBKCg, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9011)
  AUTHORS   Stellberger T, Uetz P.
  TITLE     Yeast two-hybrid vectors with C-terminal fusion of Gal4 activation 
            and DNA-binding domains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9011)
  AUTHORS   Stellberger T, Uetz P.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2009) Institut fuer Toxikologie und Genetik, 
            Forschungszentrum Karlsruhe, Hermann-von-Helmholtz-Platz 1, 
            Eggenstein-Leopoldshafen, Baden-Wuerttemberg 76344, Germany
REFERENCE   3  (bases 1 to 9011)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9011)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-FEB-2009) Institut fuer Toxikologie und Genetik, 
            Forschungszentrum Karlsruhe, Hermann-von-Helmholtz-Platz 1, 
            Eggenstein-Leopoldshafen, Baden-Wuerttemberg 76344, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        30..734
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     protein_bind    769..888
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        918..948
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1002..1658
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             2003..2305
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(2349..2473)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2487..2927
                     /label=GAL4 DNA binding domain
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     promoter        2938..2956
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2976..3005
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     terminator      complement(3093..3140)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     regulatory      3143..3310
                     /label=yADH1_terminator
                     /note="yADH1_terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(3330..3346)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3354..3370)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3378..3408)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3423..3444)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3732..4320)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    complement(4649..4696)
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(4932..5723)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(5724..5828)
                     /label=AmpR promoter
     rep_origin      complement(5855..7197)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        7456..7737
                     /label=TRP1 promoter
     CDS             7738..8409
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
     rep_origin      complement(8515..8970)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 cggtgcgggc ctcttcgcta ttacgccaga tccttttgtt gtttccgggt gtacaatatg
       61 gacttcctct tttctggcaa ccaaacccat acatcgggat tcctataata ccttcgttgg
      121 tctccctaac atgtaggtgg cggaggggag atatacaata gaacagatac cagacaagac
      181 ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg tacataacga
      241 actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca ctaccctttt
      301 tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct ttttttttct
      361 tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaaa tgatggaaga
      421 cactaaagga aaaaattaac gacaaagaca gcaccaacag atgtcgttgt tccagagctg
      481 atgaggggta tctcgaagca cacgaaactt tttccttcct tcattcacgc acactactct
      541 ctaatgagca acggtatacg gccttccttc cagttacttg aatttgaaat aaaaaaagtt
      601 tgctgtcttg ctatcaagta taaatagacc tgcaattatt aatcttttgt ttcctcgtca
      661 ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa gctataccaa
      721 gcatacaatc aactccaagc ttgaagcaag cctcctgagg taccatcaac aagtttgtac
      781 aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg
      841 cataaaaaac agactacata atactgtaaa acacaacata tccagtcata ttggcggccg
      901 cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg tggattttga
      961 gttaggatcc gtcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg
     1021 gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
     1081 cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga
     1141 ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga
     1201 tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata
     1261 gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga
     1321 gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt
     1381 acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag
     1441 ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct
     1501 tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc
     1561 tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg
     1621 aattacaaca gtactgcgat gagtggcagg gcggggcgta aagatctgga tccggcttac
     1681 taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata
     1741 ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc gtattacagt
     1801 gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt
     1861 ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg
     1921 gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct
     1981 gacgagaaca ggggctggtg aaatgcagtt taaggtttac acctataaaa gagagagccg
     2041 ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt
     2101 gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt
     2161 ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt
     2221 ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc
     2281 cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc
     2341 aggtcgacca tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat
     2401 gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct
     2461 tgtacaaagt ggttgatggt accaagatga agctactgtc ttctatcgaa caagcatgcg
     2521 atatttgccg acttaaaaag ctcaagtgct ccaaagaaaa accgaagtgc gccaagtgtc
     2581 tgaagaacaa ctgggagtgt cgctactctc ccaaaaccaa aaggtctccg ctgactaggg
     2641 cacatctgac agaagtggaa tcaaggctag aaagactgga acagctattt ctactgattt
     2701 ttcctcgaga agaccttgac atgattttga aaatggattc tttacaggat ataaaagcat
     2761 tgttaacagg attatttgta caagataatg tgaataaaga tgccgtcaca gatagattgg
     2821 cttcagtgga gactgatatg cctctaacat tgagacagca tagaataagt gcgacatcat
     2881 catcggaaga gagtagtaac aaaggtcaaa gacagttgac tgtatcgccg gaatttgtaa
     2941 tacgactcac tatagggcga gccgccatca tggaggagca gaagctgatc tcagaggagg
     3001 acctgcatat ggccatggag gccgaatttg cagcggccgc ataatcataa atcataagaa
     3061 attcgcccgg aattagcttg gctgcaagcg cgcaaaaaac ccctcaagac ccgtttagag
     3121 gccccaaggg gttatgctag tttttattat taaataagtt ataaaaaaaa taagtgtata
     3181 caaattttaa agtgactctt aggttttaaa acgaaaattc ttattcttga gtaactcttt
     3241 cctgtaggtc aggttgcttt ctcaggtata gcatgaggtc gctcttattg accacacctc
     3301 taccggcatg caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     3361 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     3421 taatgagtga ggtaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     3481 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     3541 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     3601 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     3661 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     3721 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     3781 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     3841 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     3901 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     3961 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     4021 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     4081 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     4141 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     4201 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     4261 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     4321 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     4381 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     4441 tgaagtttta aatcaatcta aagtatatat gagtaacctg aggctatggc agggcctgcc
     4501 gccccgacgt tggctgcgag ccctgggcct tcacccgaac ttggggggtg gggtggggaa
     4561 aaggaagaaa cgcgggcgta ttggccccaa tggggtctcg gtggggtatc gacagagtgc
     4621 cagccctggg accgaacccc gcgtttatga acaaacgacc caacaccgtg cgttttattc
     4681 tgtcttttta ttgccgtcat agcgcgggtt ccttccggta ttgtctcctt ccgtgtttca
     4741 gttagcctcc ccctagggtg ggcgaagaac tccagcatga gatccccgcg ctggaggatc
     4801 atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa ggcggcggtg
     4861 gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggttcattt cgaaccccag
     4921 agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga
     4981 gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca
     5041 atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag
     5101 tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgtca
     5161 tgggtcacga cgagatcctc gccgtcgggc atgctcgcct tgagcctggc gaacagttcg
     5221 gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc
     5281 atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc
     5341 ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga
     5401 gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt
     5461 cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac
     5521 gatagccgcg ctgcctcgtc ttgcagttca ttcagggcac cggacaggtc ggtcttgaca
     5581 aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt
     5641 gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg
     5701 tgcaatccat cttgttcaat catactcttc ctttttcaat attattgaag catttatcag
     5761 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     5821 gttccgcgca catttccccg aaaagtgcca cctgaacgaa gcatctgtgc ttcattttgt
     5881 agaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt
     5941 tacagaacag aaatgcaacg cgaaagcgct attttaccaa cgaagaatct gtgcttcatt
     6001 tttgtaaaac aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc
     6061 atttttacag aacagaaatg caacgcgaga gcgctatttt accaacaaag aatctatact
     6121 tcttttttgt tctacaaaaa tgcatcccga gagcgctatt tttctaacaa agcatcttag
     6181 attacttttt ttctcctttg tgcgctctat aatgcagtct cttgataact ttttgcactg
     6241 taggtccgtt aaggttagaa gaaggctact ttggtgtcta ttttctcttc cataaaaaaa
     6301 gcctgactcc acttcccgcg tttactgatt actagcgaag ctgcgggtgc attttttcaa
     6361 gataaaggca tccccgatta tattctatac cgatgtggat tgcgcatact ttgtgaacag
     6421 aaagtgatag cgttgatgat tcttcattgg tcagaaaatt atgaacggtt tcttctattt
     6481 tgtctctata tactacgtat aggaaatgtt tacattttcg tattgttttc gattcactct
     6541 atgaatagtt cttactacaa tttttttgtc taaagagtaa tactagagat aaacataaaa
     6601 aatgtagagg tcgagtttag atgcaagttc aaggagcgaa aggtggatgg gtaggttata
     6661 tagggatata gcacagagat atatagcaaa gagatacttt tgagcaatgt ttgtggaagc
     6721 ggtattcgca atattttagt agctcgttac agtccggtgc gtttttggtt ttttgaaagt
     6781 gcgtcttcag agcgcttttg gttttcaaaa gcgctctgaa gttcctatac tttctagaga
     6841 ataggaactt cggaatagga acttcaaagc gtttccgaaa acgagcgctt ccgaaaatgc
     6901 aacgcgagct gcgcacatac agctcactgt tcacgtcgca cctatatctg cgtgttgcct
     6961 gtatatatat atacatgaga agaacggcat agtgcgtgtt tatgcttaaa tgcgtactta
     7021 tatgcgtcta tttatgtagg atgaaaggta gtctagtacc tcctgtgata ttatcccatt
     7081 ccatgcgggg tatcgtatgc ttccttcagc actacccttt agctgttcta tatgctgcca
     7141 ctcctcaatt ggattagtct catccttcaa tgctatcatt tcctttgata ttggatcata
     7201 ttaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     7261 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     7321 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     7381 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
     7441 agtgcaccat agatcaacga cattactata tatataatat aggaagcatt taatagaaca
     7501 gcatcgtaat atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt
     7561 ctttattgaa aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt
     7621 tgccgattaa gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat
     7681 tggtgactat tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg
     7741 tctgttatta atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc
     7801 acagaggccg cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg
     7861 cccaatagaa agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa
     7921 gcatataaaa atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct
     7981 aaggaggatg ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga
     8041 gatgagtcgt ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta
     8101 tttccaaaag actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt
     8161 cccttgtttg attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct
     8221 gactgggttg gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg
     8281 acgccagaaa atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc
     8341 ggaggtgtgg agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat
     8401 gctaagaaat aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccga
     8461 tctatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggaaattg
     8521 taaacgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     8581 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     8641 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     8701 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     8761 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     8821 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     8881 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     8941 ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggctgc gcaactgttg
     9001 ggaagggcga t
//
