LOCUS       pGBDU-C3.        5996 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast two-hybrid """"bait"""" vector with a URA3 marker for fusing a
            gene to the GAL4 DNA binding domain. For other reading frames, use 
            pGBDU-C1 or pGBDU-C2.
ACCESSION   .
VERSION     .
KEYWORDS    pGBDU-C3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5996)
  AUTHORS   James P, Halladay J, Craig EA.
  TITLE     Genomic libraries and a host strain designed for highly efficient 
            two-hybrid selection in yeast.
  JOURNAL   Genetics 1996;144:1425-36.
  PUBMED    8978031
REFERENCE   2  (bases 1 to 5996)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5996)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; 
            date: "1996"; volume: "144"; pages: "1425-36"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5996
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..406
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             434..874
                     /label=GAL4 DNA binding domain
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     misc_feature    878..918
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     terminator      1293..1480
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     promoter        1491..1707
                     /label=URA3 promoter
     CDS             1708..2508
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     rep_origin      complement(2826..3414)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3588..4445)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4446..4550)
                     /label=AmpR promoter
     rep_origin      4832..5996
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 gcttgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc
       61 atatccgcaa tgacaaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga
      121 cagcaccaac agatgtcgtt gttccagagc tgatgagggg tatcttcgaa cacacgaaac
      181 tttttccttc cttcattcac gcacactact ctctaatgag caacggtata cggccttcct
      241 tccagttact tgaatttgaa ataaaaaaag tttgccgctt tgctatcaag tataaataga
      301 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt
      361 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gcttgaagca
      421 agcctcctga aagatgaagc tactgtcttc tatcgaacaa gcatgcgata tttgccgact
      481 taaaaagctc aagtgctcca aagaaaaacc gaagtgcgcc aagtgtctga agaacaactg
      541 ggagtgtcgc tactctccca aaaccaaaag gtctccgctg actagggcac atctgacaga
      601 agtggaatca aggctagaaa gactggaaca gctatttcta ctgatttttc ctcgagaaga
      661 ccttgacatg attttgaaaa tggattcttt acaggatata aaagcattgt taacaggatt
      721 atttgtacaa gataatgtga ataaagatgc cgtcacagat agattggctt cagtggagac
      781 tgatatgcct ctaacattga gacagcatag aataagtgcg acatcatcat cggaagagag
      841 tagtaacaaa ggtcaaagac agttgactgt atcgccggaa ttcccggggg atccatcgat
      901 gtcgacctgc agagatctat gaatcgtaga tactgaaaaa ccccgcaagt tcacttcaac
      961 tgtgcatcgt gcaccatctc aatttctttc atttatacat cgttttgcct tcttttatgt
     1021 aactatactc ctctaagttt caatcttggc catgtaacct ctgatctata gaatttttta
     1081 aatgactaga attaatgccc atcttttttt tggacctaaa ttcttcatga aaatatatta
     1141 cgagggctta ttcagaagct ttggacttct tcgccagagg tttggtcaag tctccaatca
     1201 aggttgtcgg cttgtctacc ttgccagaaa tttacgaaaa gatggaaaag ggtcaaatcg
     1261 ttggtagata cgttgttgac acttctaaat aagcgaattt cttatgattt atgattttta
     1321 ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt
     1381 taaaacgaaa attcttgttc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg
     1441 tatagcatga ggtcgctctt attgaccaca cctctaccgg catgcagctt ttcaattcaa
     1501 ttcatcattt tttttttatt cttttttttg atttcggttt ctttgaaatt tttttgattc
     1561 ggtaatctcc gaacagaagg aagaacgaag gaaggagcac agacttagat tggtatatat
     1621 acgcatatgt agtgttgaag aaacatgaaa ttgcccagta ttcttaaccc aactgcacag
     1681 aacaaaaacc ggaaacgaag ataaatcatg tcgaaagcta catataagga acgtgctgct
     1741 actcatccta gtcctgttgc tgccaagcta tttaatatca tgcacgaaaa gcaaacaaac
     1801 ttgtgtgctt cattggatgt tcgtaccacc aaggaattac tggagttagt tgaagcatta
     1861 ggtcccaaaa tttgtttact aaaaacacat gtggatatct tgactgattt ttccatggag
     1921 ggcacagtta agccgctaaa ggcattatcc gccaagtaca attttttact cttcgaagac
     1981 agaaaatttg ctgacattgg taatacagtc aaattgcagt actctgcggg tgtatacaga
     2041 atagcagaat gggcagacat tacgaatgca cacggtgtgg tgggcccagg tattgttagc
     2101 ggtttgaagc aggcggcaga agaagtaaca aaggaaccta gaggcctttt gatgttagca
     2161 gaattgtcat gcaagggctc cctatctact ggagaatata ctaagggtac tgttgacatt
     2221 gcgaagagcg acaaagattt tgttatcggc tttattgctc aaagagacat gggtggaaga
     2281 gatgaaggtt acgattggtt gattatgaca cccggtgtgg gtttagatga caagggagac
     2341 gcattgggtc aacagtatag aaccgtggat gatgtggtct ctacaggatc tgacattatt
     2401 attgttggaa gaggactatt tgcaaaggga agggatgcta aggtagaggg tgaacgttac
     2461 agaaaagcag gctgggaagc atatttgaga agatgcggcc agcaaaacta aaaaactgta
     2521 ttataagtaa atgcatgtat actaaactca caaattagag cttcaattta attatatcag
     2581 ttattacccc tggattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     2641 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     2701 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     2761 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     2821 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     2881 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     2941 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     3001 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     3061 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     3121 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     3181 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3241 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     3301 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3361 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3421 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     3481 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     3541 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     3601 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     3661 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     3721 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     3781 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     3841 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     3901 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     3961 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     4021 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     4081 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     4141 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     4201 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     4261 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     4321 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     4381 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     4441 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     4501 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     4561 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     4621 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
     4681 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     4741 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
     4801 tcagagcaga ttgtactgag agtgcaccat aacgcattta agcataaaca cgcactatgc
     4861 cgttcttctc atgtatatat atatacaggc aacacgcaga tataggtgcg acgtgaacag
     4921 tgagctgtat gtgcgcagct cgcgttgcat tttcggaagc gctcgttttc ggaaacgctt
     4981 tgaagttcct attccgaagt tcctattctc tagctagaaa gtataggaac ttcagagcgc
     5041 ttttgaaaac caaaagcgct ctgaagacgc actttcaaaa aaccaaaaac gcaccggact
     5101 gtaacgagct actaaaatat tgcgaatacc gcttccacaa acattgctca aaagtatctc
     5161 tttgctatat atctctgtgc tatatcccta tataacctac ccatccacct ttcgctcctt
     5221 gaacttgcat ctaaactcga cctctacatt ttttatgttt atctctagta ttactcttta
     5281 gacaaaaaaa ttgtagtaag aactattcat agagtgaatc gaaaacaata cgaaaatgta
     5341 aacatttcct atacgtagta tatagagaca aaatagaaga aaccgttcat aattttctga
     5401 ccaatgaaga atcatcaacg ctatcacttt ctgttcacaa agtatgcgca atccacatcg
     5461 gtatagaata taatcgggga tgcctttatc ttgaaaaaat gcacccgcag cttcgctagt
     5521 aatcagtaaa cgcgggaagt ggagtcaggc tttttttatg gaagagaaaa tagacaccaa
     5581 agtagccttc ttctaacctt aacggaccta cagtgcaaaa agttatcaag agactgcatt
     5641 atagagcgca caaaggagaa aaaaagtaat ctaagatgct ttgttagaaa aatagcgctc
     5701 tcgggatgca tttttgtaga acaaaaaaga agtatagatt ctttgttggt aaaatagcgc
     5761 tctcgcgttg catttctgtt ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag
     5821 cgctctcgcg ttgcattttt gttttacaaa aatgaagcac agattcttcg ttggtaaaat
     5881 agcgctttcg cgttgcattt ctgttctgta aaaatgcagc tcagattctt tgtttgaaaa
     5941 attagcgctc tcgcgttgca tttttgttct acaaaatgaa gcacagatgc ttcgtt
//
