LOCUS       pGBDU-C1.        5997 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast two-hybrid """"bait"""" vector with a URA3 marker for fusing a
            gene to the GAL4 DNA binding domain. For other reading frames, use 
            pGBDU-C2 or pGBDU-C3.
ACCESSION   .
VERSION     .
KEYWORDS    pGBDU-C1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5997)
  AUTHORS   James P, Halladay J, Craig EA.
  TITLE     Genomic libraries and a host strain designed for highly efficient 
            two-hybrid selection in yeast.
  JOURNAL   Genetics 1996;144:1425-36.
  PUBMED    8978031
REFERENCE   2  (bases 1 to 5997)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5997)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; 
            date: "1996"; volume: "144"; pages: "1425-36"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5997
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..406
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             434..874
                     /label=GAL4 DNA binding domain
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     misc_feature    878..919
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     terminator      1294..1481
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     promoter        1492..1708
                     /label=URA3 promoter
     CDS             1709..2509
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     rep_origin      complement(2827..3415)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3589..4446)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4447..4551)
                     /label=AmpR promoter
     rep_origin      4833..5997
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 gcttgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc
       61 atatccgcaa tgacaaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga
      121 cagcaccaac agatgtcgtt gttccagagc tgatgagggg tatcttcgaa cacacgaaac
      181 tttttccttc cttcattcac gcacactact ctctaatgag caacggtata cggccttcct
      241 tccagttact tgaatttgaa ataaaaaaag tttgccgctt tgctatcaag tataaataga
      301 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt
      361 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gcttgaagca
      421 agcctcctga aagatgaagc tactgtcttc tatcgaacaa gcatgcgata tttgccgact
      481 taaaaagctc aagtgctcca aagaaaaacc gaagtgcgcc aagtgtctga agaacaactg
      541 ggagtgtcgc tactctccca aaaccaaaag gtctccgctg actagggcac atctgacaga
      601 agtggaatca aggctagaaa gactggaaca gctatttcta ctgatttttc ctcgagaaga
      661 ccttgacatg attttgaaaa tggattcttt acaggatata aaagcattgt taacaggatt
      721 atttgtacaa gataatgtga ataaagatgc cgtcacagat agattggctt cagtggagac
      781 tgatatgcct ctaacattga gacagcatag aataagtgcg acatcatcat cggaagagag
      841 tagtaacaaa ggtcaaagac agttgactgt atcgccggaa ttccccgggg gatccatcga
      901 tgtcgacctg cagagatcta tgaatcgtag atactgaaaa accccgcaag ttcacttcaa
      961 ctgtgcatcg tgcaccatct caatttcttt catttataca tcgttttgcc ttcttttatg
     1021 taactatact cctctaagtt tcaatcttgg ccatgtaacc tctgatctat agaatttttt
     1081 aaatgactag aattaatgcc catctttttt ttggacctaa attcttcatg aaaatatatt
     1141 acgagggctt attcagaagc tttggacttc ttcgccagag gtttggtcaa gtctccaatc
     1201 aaggttgtcg gcttgtctac cttgccagaa atttacgaaa agatggaaaa gggtcaaatc
     1261 gttggtagat acgttgttga cacttctaaa taagcgaatt tcttatgatt tatgattttt
     1321 attattaaat aagttataaa aaaaataagt gtatacaaat tttaaagtga ctcttaggtt
     1381 ttaaaacgaa aattcttgtt cttgagtaac tctttcctgt aggtcaggtt gctttctcag
     1441 gtatagcatg aggtcgctct tattgaccac acctctaccg gcatgcagct tttcaattca
     1501 attcatcatt ttttttttat tctttttttt gatttcggtt tctttgaaat ttttttgatt
     1561 cggtaatctc cgaacagaag gaagaacgaa ggaaggagca cagacttaga ttggtatata
     1621 tacgcatatg tagtgttgaa gaaacatgaa attgcccagt attcttaacc caactgcaca
     1681 gaacaaaaac cggaaacgaa gataaatcat gtcgaaagct acatataagg aacgtgctgc
     1741 tactcatcct agtcctgttg ctgccaagct atttaatatc atgcacgaaa agcaaacaaa
     1801 cttgtgtgct tcattggatg ttcgtaccac caaggaatta ctggagttag ttgaagcatt
     1861 aggtcccaaa atttgtttac taaaaacaca tgtggatatc ttgactgatt tttccatgga
     1921 gggcacagtt aagccgctaa aggcattatc cgccaagtac aattttttac tcttcgaaga
     1981 cagaaaattt gctgacattg gtaatacagt caaattgcag tactctgcgg gtgtatacag
     2041 aatagcagaa tgggcagaca ttacgaatgc acacggtgtg gtgggcccag gtattgttag
     2101 cggtttgaag caggcggcag aagaagtaac aaaggaacct agaggccttt tgatgttagc
     2161 agaattgtca tgcaagggct ccctatctac tggagaatat actaagggta ctgttgacat
     2221 tgcgaagagc gacaaagatt ttgttatcgg ctttattgct caaagagaca tgggtggaag
     2281 agatgaaggt tacgattggt tgattatgac acccggtgtg ggtttagatg acaagggaga
     2341 cgcattgggt caacagtata gaaccgtgga tgatgtggtc tctacaggat ctgacattat
     2401 tattgttgga agaggactat ttgcaaaggg aagggatgct aaggtagagg gtgaacgtta
     2461 cagaaaagca ggctgggaag catatttgag aagatgcggc cagcaaaact aaaaaactgt
     2521 attataagta aatgcatgta tactaaactc acaaattaga gcttcaattt aattatatca
     2581 gttattaccc ctggattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     2641 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     2701 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     2761 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     2821 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     2881 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     2941 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     3001 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     3061 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     3121 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     3181 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     3241 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     3301 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     3361 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     3421 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     3481 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     3541 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     3601 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     3661 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     3721 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     3781 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     3841 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     3901 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     3961 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     4021 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     4081 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     4141 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     4201 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     4261 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     4321 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     4381 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     4441 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     4501 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     4561 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     4621 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     4681 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     4741 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     4801 atcagagcag attgtactga gagtgcacca taacgcattt aagcataaac acgcactatg
     4861 ccgttcttct catgtatata tatatacagg caacacgcag atataggtgc gacgtgaaca
     4921 gtgagctgta tgtgcgcagc tcgcgttgca ttttcggaag cgctcgtttt cggaaacgct
     4981 ttgaagttcc tattccgaag ttcctattct ctagctagaa agtataggaa cttcagagcg
     5041 cttttgaaaa ccaaaagcgc tctgaagacg cactttcaaa aaaccaaaaa cgcaccggac
     5101 tgtaacgagc tactaaaata ttgcgaatac cgcttccaca aacattgctc aaaagtatct
     5161 ctttgctata tatctctgtg ctatatccct atataaccta cccatccacc tttcgctcct
     5221 tgaacttgca tctaaactcg acctctacat tttttatgtt tatctctagt attactcttt
     5281 agacaaaaaa attgtagtaa gaactattca tagagtgaat cgaaaacaat acgaaaatgt
     5341 aaacatttcc tatacgtagt atatagagac aaaatagaag aaaccgttca taattttctg
     5401 accaatgaag aatcatcaac gctatcactt tctgttcaca aagtatgcgc aatccacatc
     5461 ggtatagaat ataatcgggg atgcctttat cttgaaaaaa tgcacccgca gcttcgctag
     5521 taatcagtaa acgcgggaag tggagtcagg ctttttttat ggaagagaaa atagacacca
     5581 aagtagcctt cttctaacct taacggacct acagtgcaaa aagttatcaa gagactgcat
     5641 tatagagcgc acaaaggaga aaaaaagtaa tctaagatgc tttgttagaa aaatagcgct
     5701 ctcgggatgc atttttgtag aacaaaaaag aagtatagat tctttgttgg taaaatagcg
     5761 ctctcgcgtt gcatttctgt tctgtaaaaa tgcagctcag attctttgtt tgaaaaatta
     5821 gcgctctcgc gttgcatttt tgttttacaa aaatgaagca cagattcttc gttggtaaaa
     5881 tagcgctttc gcgttgcatt tctgttctgt aaaaatgcag ctcagattct ttgtttgaaa
     5941 aattagcgct ctcgcgttgc atttttgttc tacaaaatga agcacagatg cttcgtt
//
