LOCUS       pGBD-C3.        5896 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast two-hybrid """"bait"""" vector for fusing a gene to the GAL4 
            DNA binding domain. For other reading frames, use pGBD-C1 or 
            pGBD-C2.
ACCESSION   .
VERSION     .
KEYWORDS    pGBD-C3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5896)
  AUTHORS   James P, Halladay J, Craig EA.
  TITLE     Genomic libraries and a host strain designed for highly efficient 
            two-hybrid selection in yeast.
  JOURNAL   Genetics 1996;144:1425-36.
  PUBMED    8978031
REFERENCE   2  (bases 1 to 5896)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5896)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; 
            date: "1996"; volume: "144"; pages: "1425-36"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..406
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             434..874
                     /label=GAL4 DNA binding domain
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     misc_feature    878..918
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     terminator      1293..1480
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(1536..2207)
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
     promoter        complement(2208..2309)
                     /label=TRP1 promoter
     primer_bind     complement(2324..2340)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2348..2364)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2372..2402)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2417..2438)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2726..3314)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3488..4345)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4346..4450)
                     /label=AmpR promoter
     rep_origin      4732..5896
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 gcttgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc
       61 atatccgcaa tgacaaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga
      121 cagcaccaac agatgtcgtt gttccagagc tgatgagggg tatcttcgaa cacacgaaac
      181 tttttccttc cttcattcac gcacactact ctctaatgag caacggtata cggccttcct
      241 tccagttact tgaatttgaa ataaaaaaag tttgccgctt tgctatcaag tataaataga
      301 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt
      361 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gcttgaagca
      421 agcctcctga aagatgaagc tactgtcttc tatcgaacaa gcatgcgata tttgccgact
      481 taaaaagctc aagtgctcca aagaaaaacc gaagtgcgcc aagtgtctga agaacaactg
      541 ggagtgtcgc tactctccca aaaccaaaag gtctccgctg actagggcac atctgacaga
      601 agtggaatca aggctagaaa gactggaaca gctatttcta ctgatttttc ctcgagaaga
      661 ccttgacatg attttgaaaa tggattcttt acaggatata aaagcattgt taacaggatt
      721 atttgtacaa gataatgtga ataaagatgc cgtcacagat agattggctt cagtggagac
      781 tgatatgcct ctaacattga gacagcatag aataagtgcg acatcatcat cggaagagag
      841 tagtaacaaa ggtcaaagac agttgactgt atcgccggaa ttcccggggg atccatcgat
      901 gtcgacctgc agagatctat gaatcgtaga tactgaaaaa ccccgcaagt tcacttcaac
      961 tgtgcatcgt gcaccatctc aatttctttc atttatacat cgttttgcct tcttttatgt
     1021 aactatactc ctctaagttt caatcttggc catgtaacct ctgatctata gaatttttta
     1081 aatgactaga attaatgccc atcttttttt tggacctaaa ttcttcatga aaatatatta
     1141 cgagggctta ttcagaagct ttggacttct tcgccagagg tttggtcaag tctccaatca
     1201 aggttgtcgg cttgtctacc ttgccagaaa tttacgaaaa gatggaaaag ggtcaaatcg
     1261 ttggtagata cgttgttgac acttctaaat aagcgaattt cttatgattt atgattttta
     1321 ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt
     1381 taaaacgaaa attcttgttc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg
     1441 tatagcatga ggtcgctctt attgaccaca cctctaccgg catgccggca agtgcacaaa
     1501 caatacttaa ataaatacta ctcagtaata acctatttct tagcattttt gacgaaattt
     1561 gctattttgt tagagtcttt tacaccattt gtctccacac ctccgcttac atcaacacca
     1621 ataacgccat ttaatctaag cgcatcacca acattttctg gcgtcagtcc accagctaac
     1681 ataaaatgta agctttcggg gctctcttgc cttccaaccc agtcagaaat cgagttccaa
     1741 tccaaaagtt cacctgtccc acctgcttct gaatcaaaca agggaataaa cgaatgaggt
     1801 ttctgtgaag ctgcactgag tagtatgttg cagtcttttg gaaatacgag tcttttaata
     1861 actggcaaac cgaggaactc ttggtattct tgccacgact catctccatg cagttggacg
     1921 atatcaatgc cgtaatcatt gaccagagcc aaaacatcct ccttaggttg attacgaaac
     1981 acgccaacca agtatttcgg agtgcctgaa ctatttttat atgcttttac aagacttgaa
     2041 attttccttg caataaccgg gtcaattgtt ctctttctat tgggcacaca tataataccc
     2101 agcaagtcag catcggaatc tagagcacat tctgcggcct ctgtgctctg caagccgcaa
     2161 actttcacca atggaccaga actacctgtg aaattaataa cagacatact ccaagctgcc
     2221 tttgtgtgct taatcacgta tactcacgtg ctcaatagtc accaatgccc tccctcttgg
     2281 ccctctcctt ttcttttttc gaccgaatta attcgtaatc atggtcatag ctgtttcctg
     2341 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     2401 aagcctgggg tgcctaatga gtgaggtaac tcacattaat tgcgttgcgc tcactgcccg
     2461 ctttccagtc gggaaacctg tcgtgccagc tggattaatg aatcggccaa cgcgcgggga
     2521 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     2581 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     2641 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     2701 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     2761 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     2821 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     2881 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     2941 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3001 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3061 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3121 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     3181 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     3241 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     3301 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     3361 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     3421 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     3481 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     3541 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     3601 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     3661 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     3721 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     3781 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     3841 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     3901 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     3961 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     4021 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     4081 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     4141 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     4201 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     4261 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     4321 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     4381 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     4441 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     4501 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     4561 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     4621 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     4681 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat aacgcattta
     4741 agcataaaca cgcactatgc cgttcttctc atgtatatat atatacaggc aacacgcaga
     4801 tataggtgcg acgtgaacag tgagctgtat gtgcgcagct cgcgttgcat tttcggaagc
     4861 gctcgttttc ggaaacgctt tgaagttcct attccgaagt tcctattctc tagctagaaa
     4921 gtataggaac ttcagagcgc ttttgaaaac caaaagcgct ctgaagacgc actttcaaaa
     4981 aaccaaaaac gcaccggact gtaacgagct actaaaatat tgcgaatacc gcttccacaa
     5041 acattgctca aaagtatctc tttgctatat atctctgtgc tatatcccta tataacctac
     5101 ccatccacct ttcgctcctt gaacttgcat ctaaactcga cctctacatt ttttatgttt
     5161 atctctagta ttactcttta gacaaaaaaa ttgtagtaag aactattcat agagtgaatc
     5221 gaaaacaata cgaaaatgta aacatttcct atacgtagta tatagagaca aaatagaaga
     5281 aaccgttcat aattttctga ccaatgaaga atcatcaacg ctatcacttt ctgttcacaa
     5341 agtatgcgca atccacatcg gtatagaata taatcgggga tgcctttatc ttgaaaaaat
     5401 gcacccgcag cttcgctagt aatcagtaaa cgcgggaagt ggagtcaggc tttttttatg
     5461 gaagagaaaa tagacaccaa agtagccttc ttctaacctt aacggaccta cagtgcaaaa
     5521 agttatcaag agactgcatt atagagcgca caaaggagaa aaaaagtaat ctaagatgct
     5581 ttgttagaaa aatagcgctc tcgggatgca tttttgtaga acaaaaaaga agtatagatt
     5641 ctttgttggt aaaatagcgc tctcgcgttg catttctgtt ctgtaaaaat gcagctcaga
     5701 ttctttgttt gaaaaattag cgctctcgcg ttgcattttt gttttacaaa aatgaagcac
     5761 agattcttcg ttggtaaaat agcgctttcg cgttgcattt ctgttctgta aaaatgcagc
     5821 tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgttct acaaaatgaa
     5881 gcacagatgc ttcgtt
//
