LOCUS       pGBD-C2.        5895 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast two-hybrid """"bait"""" vector for fusing a gene to the GAL4 
            DNA binding domain. For other reading frames, use pGBD-C1 or 
            pGBD-C3.
ACCESSION   .
VERSION     .
KEYWORDS    pGBD-C2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5895)
  AUTHORS   James P, Halladay J, Craig EA.
  TITLE     Genomic libraries and a host strain designed for highly efficient 
            two-hybrid selection in yeast.
  JOURNAL   Genetics 1996;144:1425-36.
  PUBMED    8978031
REFERENCE   2  (bases 1 to 5895)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5895)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; 
            date: "1996"; volume: "144"; pages: "1425-36"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..406
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             434..874
                     /label=GAL4 DNA binding domain
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     misc_feature    878..917
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     terminator      1292..1479
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(1535..2206)
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
     promoter        complement(2207..2308)
                     /label=TRP1 promoter
     primer_bind     complement(2323..2339)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2347..2363)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2371..2401)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2416..2437)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2725..3313)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3487..4344)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4345..4449)
                     /label=AmpR promoter
     rep_origin      4731..5895
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 gcttgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc
       61 atatccgcaa tgacaaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga
      121 cagcaccaac agatgtcgtt gttccagagc tgatgagggg tatcttcgaa cacacgaaac
      181 tttttccttc cttcattcac gcacactact ctctaatgag caacggtata cggccttcct
      241 tccagttact tgaatttgaa ataaaaaaag tttgccgctt tgctatcaag tataaataga
      301 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt
      361 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gcttgaagca
      421 agcctcctga aagatgaagc tactgtcttc tatcgaacaa gcatgcgata tttgccgact
      481 taaaaagctc aagtgctcca aagaaaaacc gaagtgcgcc aagtgtctga agaacaactg
      541 ggagtgtcgc tactctccca aaaccaaaag gtctccgctg actagggcac atctgacaga
      601 agtggaatca aggctagaaa gactggaaca gctatttcta ctgatttttc ctcgagaaga
      661 ccttgacatg attttgaaaa tggattcttt acaggatata aaagcattgt taacaggatt
      721 atttgtacaa gataatgtga ataaagatgc cgtcacagat agattggctt cagtggagac
      781 tgatatgcct ctaacattga gacagcatag aataagtgcg acatcatcat cggaagagag
      841 tagtaacaaa ggtcaaagac agttgactgt atcgccggaa ttcccgggga tccatcgatg
      901 tcgacctgca gagatctatg aatcgtagat actgaaaaac cccgcaagtt cacttcaact
      961 gtgcatcgtg caccatctca atttctttca tttatacatc gttttgcctt cttttatgta
     1021 actatactcc tctaagtttc aatcttggcc atgtaacctc tgatctatag aattttttaa
     1081 atgactagaa ttaatgccca tctttttttt ggacctaaat tcttcatgaa aatatattac
     1141 gagggcttat tcagaagctt tggacttctt cgccagaggt ttggtcaagt ctccaatcaa
     1201 ggttgtcggc ttgtctacct tgccagaaat ttacgaaaag atggaaaagg gtcaaatcgt
     1261 tggtagatac gttgttgaca cttctaaata agcgaatttc ttatgattta tgatttttat
     1321 tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt
     1381 aaaacgaaaa ttcttgttct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt
     1441 atagcatgag gtcgctctta ttgaccacac ctctaccggc atgccggcaa gtgcacaaac
     1501 aatacttaaa taaatactac tcagtaataa cctatttctt agcatttttg acgaaatttg
     1561 ctattttgtt agagtctttt acaccatttg tctccacacc tccgcttaca tcaacaccaa
     1621 taacgccatt taatctaagc gcatcaccaa cattttctgg cgtcagtcca ccagctaaca
     1681 taaaatgtaa gctttcgggg ctctcttgcc ttccaaccca gtcagaaatc gagttccaat
     1741 ccaaaagttc acctgtccca cctgcttctg aatcaaacaa gggaataaac gaatgaggtt
     1801 tctgtgaagc tgcactgagt agtatgttgc agtcttttgg aaatacgagt cttttaataa
     1861 ctggcaaacc gaggaactct tggtattctt gccacgactc atctccatgc agttggacga
     1921 tatcaatgcc gtaatcattg accagagcca aaacatcctc cttaggttga ttacgaaaca
     1981 cgccaaccaa gtatttcgga gtgcctgaac tatttttata tgcttttaca agacttgaaa
     2041 ttttccttgc aataaccggg tcaattgttc tctttctatt gggcacacat ataataccca
     2101 gcaagtcagc atcggaatct agagcacatt ctgcggcctc tgtgctctgc aagccgcaaa
     2161 ctttcaccaa tggaccagaa ctacctgtga aattaataac agacatactc caagctgcct
     2221 ttgtgtgctt aatcacgtat actcacgtgc tcaatagtca ccaatgccct ccctcttggc
     2281 cctctccttt tcttttttcg accgaattaa ttcgtaatca tggtcatagc tgtttcctgt
     2341 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     2401 agcctggggt gcctaatgag tgaggtaact cacattaatt gcgttgcgct cactgcccgc
     2461 tttccagtcg ggaaacctgt cgtgccagct ggattaatga atcggccaac gcgcggggag
     2521 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     2581 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     2641 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     2701 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     2761 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     2821 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     2881 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     2941 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     3001 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     3061 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     3121 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     3181 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     3241 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     3301 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     3361 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     3421 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     3481 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     3541 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     3601 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     3661 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     3721 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     3781 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     3841 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     3901 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     3961 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     4021 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     4081 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     4141 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     4201 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     4261 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     4321 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     4381 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     4441 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     4501 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
     4561 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     4621 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     4681 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata acgcatttaa
     4741 gcataaacac gcactatgcc gttcttctca tgtatatata tatacaggca acacgcagat
     4801 ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc gcgttgcatt ttcggaagcg
     4861 ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt cctattctct agctagaaag
     4921 tataggaact tcagagcgct tttgaaaacc aaaagcgctc tgaagacgca ctttcaaaaa
     4981 accaaaaacg caccggactg taacgagcta ctaaaatatt gcgaataccg cttccacaaa
     5041 cattgctcaa aagtatctct ttgctatata tctctgtgct atatccctat ataacctacc
     5101 catccacctt tcgctccttg aacttgcatc taaactcgac ctctacattt tttatgttta
     5161 tctctagtat tactctttag acaaaaaaat tgtagtaaga actattcata gagtgaatcg
     5221 aaaacaatac gaaaatgtaa acatttccta tacgtagtat atagagacaa aatagaagaa
     5281 accgttcata attttctgac caatgaagaa tcatcaacgc tatcactttc tgttcacaaa
     5341 gtatgcgcaa tccacatcgg tatagaatat aatcggggat gcctttatct tgaaaaaatg
     5401 cacccgcagc ttcgctagta atcagtaaac gcgggaagtg gagtcaggct ttttttatgg
     5461 aagagaaaat agacaccaaa gtagccttct tctaacctta acggacctac agtgcaaaaa
     5521 gttatcaaga gactgcatta tagagcgcac aaaggagaaa aaaagtaatc taagatgctt
     5581 tgttagaaaa atagcgctct cgggatgcat ttttgtagaa caaaaaagaa gtatagattc
     5641 tttgttggta aaatagcgct ctcgcgttgc atttctgttc tgtaaaaatg cagctcagat
     5701 tctttgtttg aaaaattagc gctctcgcgt tgcatttttg ttttacaaaa atgaagcaca
     5761 gattcttcgt tggtaaaata gcgctttcgc gttgcatttc tgttctgtaa aaatgcagct
     5821 cagattcttt gtttgaaaaa ttagcgctct cgcgttgcat ttttgttcta caaaatgaag
     5881 cacagatgct tcgtt
//
