LOCUS       pGBD-C1.        5897 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast two-hybrid """"bait"""" vector for fusing a gene to the GAL4 
            DNA binding domain. For other reading frames, use pGBD-C2 or 
            pGBD-C3.
ACCESSION   .
VERSION     .
KEYWORDS    pGBD-C1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5897)
  AUTHORS   James P, Halladay J, Craig EA.
  TITLE     Genomic libraries and a host strain designed for highly efficient 
            two-hybrid selection in yeast.
  JOURNAL   Genetics 1996;144:1425-36.
  PUBMED    8978031
REFERENCE   2  (bases 1 to 5897)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5897)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; 
            date: "1996"; volume: "144"; pages: "1425-36"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5897
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..406
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             434..874
                     /label=GAL4 DNA binding domain
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     misc_feature    878..919
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     terminator      1294..1481
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(1537..2208)
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
     promoter        complement(2209..2310)
                     /label=TRP1 promoter
     primer_bind     complement(2325..2341)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2349..2365)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2373..2403)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2418..2439)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2727..3315)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3489..4346)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4347..4451)
                     /label=AmpR promoter
     rep_origin      4733..5897
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 gcttgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc
       61 atatccgcaa tgacaaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga
      121 cagcaccaac agatgtcgtt gttccagagc tgatgagggg tatcttcgaa cacacgaaac
      181 tttttccttc cttcattcac gcacactact ctctaatgag caacggtata cggccttcct
      241 tccagttact tgaatttgaa ataaaaaaag tttgccgctt tgctatcaag tataaataga
      301 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt
      361 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gcttgaagca
      421 agcctcctga aagatgaagc tactgtcttc tatcgaacaa gcatgcgata tttgccgact
      481 taaaaagctc aagtgctcca aagaaaaacc gaagtgcgcc aagtgtctga agaacaactg
      541 ggagtgtcgc tactctccca aaaccaaaag gtctccgctg actagggcac atctgacaga
      601 agtggaatca aggctagaaa gactggaaca gctatttcta ctgatttttc ctcgagaaga
      661 ccttgacatg attttgaaaa tggattcttt acaggatata aaagcattgt taacaggatt
      721 atttgtacaa gataatgtga ataaagatgc cgtcacagat agattggctt cagtggagac
      781 tgatatgcct ctaacattga gacagcatag aataagtgcg acatcatcat cggaagagag
      841 tagtaacaaa ggtcaaagac agttgactgt atcgccggaa ttccccgggg gatccatcga
      901 tgtcgacctg cagagatcta tgaatcgtag atactgaaaa accccgcaag ttcacttcaa
      961 ctgtgcatcg tgcaccatct caatttcttt catttataca tcgttttgcc ttcttttatg
     1021 taactatact cctctaagtt tcaatcttgg ccatgtaacc tctgatctat agaatttttt
     1081 aaatgactag aattaatgcc catctttttt ttggacctaa attcttcatg aaaatatatt
     1141 acgagggctt attcagaagc tttggacttc ttcgccagag gtttggtcaa gtctccaatc
     1201 aaggttgtcg gcttgtctac cttgccagaa atttacgaaa agatggaaaa gggtcaaatc
     1261 gttggtagat acgttgttga cacttctaaa taagcgaatt tcttatgatt tatgattttt
     1321 attattaaat aagttataaa aaaaataagt gtatacaaat tttaaagtga ctcttaggtt
     1381 ttaaaacgaa aattcttgtt cttgagtaac tctttcctgt aggtcaggtt gctttctcag
     1441 gtatagcatg aggtcgctct tattgaccac acctctaccg gcatgccggc aagtgcacaa
     1501 acaatactta aataaatact actcagtaat aacctatttc ttagcatttt tgacgaaatt
     1561 tgctattttg ttagagtctt ttacaccatt tgtctccaca cctccgctta catcaacacc
     1621 aataacgcca tttaatctaa gcgcatcacc aacattttct ggcgtcagtc caccagctaa
     1681 cataaaatgt aagctttcgg ggctctcttg ccttccaacc cagtcagaaa tcgagttcca
     1741 atccaaaagt tcacctgtcc cacctgcttc tgaatcaaac aagggaataa acgaatgagg
     1801 tttctgtgaa gctgcactga gtagtatgtt gcagtctttt ggaaatacga gtcttttaat
     1861 aactggcaaa ccgaggaact cttggtattc ttgccacgac tcatctccat gcagttggac
     1921 gatatcaatg ccgtaatcat tgaccagagc caaaacatcc tccttaggtt gattacgaaa
     1981 cacgccaacc aagtatttcg gagtgcctga actattttta tatgctttta caagacttga
     2041 aattttcctt gcaataaccg ggtcaattgt tctctttcta ttgggcacac atataatacc
     2101 cagcaagtca gcatcggaat ctagagcaca ttctgcggcc tctgtgctct gcaagccgca
     2161 aactttcacc aatggaccag aactacctgt gaaattaata acagacatac tccaagctgc
     2221 ctttgtgtgc ttaatcacgt atactcacgt gctcaatagt caccaatgcc ctccctcttg
     2281 gccctctcct tttctttttt cgaccgaatt aattcgtaat catggtcata gctgtttcct
     2341 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     2401 aaagcctggg gtgcctaatg agtgaggtaa ctcacattaa ttgcgttgcg ctcactgccc
     2461 gctttccagt cgggaaacct gtcgtgccag ctggattaat gaatcggcca acgcgcgggg
     2521 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     2581 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     2641 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     2701 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     2761 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     2821 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     2881 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     2941 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     3001 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     3061 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     3121 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     3181 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     3241 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     3301 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     3361 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     3421 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     3481 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     3541 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     3601 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     3661 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     3721 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     3781 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     3841 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     3901 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     3961 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     4021 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     4081 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     4141 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     4201 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     4261 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     4321 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     4381 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     4441 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     4501 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     4561 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     4621 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     4681 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca taacgcattt
     4741 aagcataaac acgcactatg ccgttcttct catgtatata tatatacagg caacacgcag
     4801 atataggtgc gacgtgaaca gtgagctgta tgtgcgcagc tcgcgttgca ttttcggaag
     4861 cgctcgtttt cggaaacgct ttgaagttcc tattccgaag ttcctattct ctagctagaa
     4921 agtataggaa cttcagagcg cttttgaaaa ccaaaagcgc tctgaagacg cactttcaaa
     4981 aaaccaaaaa cgcaccggac tgtaacgagc tactaaaata ttgcgaatac cgcttccaca
     5041 aacattgctc aaaagtatct ctttgctata tatctctgtg ctatatccct atataaccta
     5101 cccatccacc tttcgctcct tgaacttgca tctaaactcg acctctacat tttttatgtt
     5161 tatctctagt attactcttt agacaaaaaa attgtagtaa gaactattca tagagtgaat
     5221 cgaaaacaat acgaaaatgt aaacatttcc tatacgtagt atatagagac aaaatagaag
     5281 aaaccgttca taattttctg accaatgaag aatcatcaac gctatcactt tctgttcaca
     5341 aagtatgcgc aatccacatc ggtatagaat ataatcgggg atgcctttat cttgaaaaaa
     5401 tgcacccgca gcttcgctag taatcagtaa acgcgggaag tggagtcagg ctttttttat
     5461 ggaagagaaa atagacacca aagtagcctt cttctaacct taacggacct acagtgcaaa
     5521 aagttatcaa gagactgcat tatagagcgc acaaaggaga aaaaaagtaa tctaagatgc
     5581 tttgttagaa aaatagcgct ctcgggatgc atttttgtag aacaaaaaag aagtatagat
     5641 tctttgttgg taaaatagcg ctctcgcgtt gcatttctgt tctgtaaaaa tgcagctcag
     5701 attctttgtt tgaaaaatta gcgctctcgc gttgcatttt tgttttacaa aaatgaagca
     5761 cagattcttc gttggtaaaa tagcgctttc gcgttgcatt tctgttctgt aaaaatgcag
     5821 ctcagattct ttgtttgaaa aattagcgct ctcgcgttgc atttttgttc tacaaaatga
     5881 agcacagatg cttcgtt
//
