LOCUS       Exported                3149 bp DNA     circular SYN 02-SEP-2024
DEFINITION  Pichia pastoris vector for constitutive expression of a secreted 
            protein. For other reading frames, use pGAPZ-alpha B or pGAPZ-alpha 
            C.
ACCESSION   .
VERSION     .
KEYWORDS    pGAPZ-A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3149)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3149)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3149)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     Linearize within the GAP promoter for integration.
FEATURES             Location/Qualifiers
     source          1..3149
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..483
                     /label=GAP promoter
                     /note="promoter for Pichia pastoris
                     glyceraldehyde-3-phosphate dehydrogenase"
     CDS             493..759
                     /label=alpha-factor secretion signal
                     /note="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
     misc_feature    760..828
                     /label=MCS
                     /note="MCS"
                     /note="MCS;multiple cloning site"
     CDS             827..856
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             872..889
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      968..1214
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        1229..1642
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        1649..1696
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             1715..2086
                     /label=BleoR
                     /note="antibiotic-binding protein"
     terminator      2155..2402
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(2477..3065)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatcttttt tgtagaaatg tcttggtgtc ctcgtccaat caggtagcca tctctgaaat
       61 atctggctcc gttgcaactc cgaacgacct gctggcaacg taaaattctc cggggtaaaa
      121 cttaaatgtg gagtaatgga accagaaacg tctcttccct tctctctcct tccaccgccc
      181 gttaccgtcc ctaggaaatt ttactctgct ggagagcttc ttctacggcc cccttgcagc
      241 aatgctcttc ccagcattac gttgcgggta aaacggaggt cgtgtacccg acctagcagc
      301 ccagggatgg aaaagtcccg gccgtcgctg gcaataatag cgggcggacg catgtcatga
      361 gattattgga aaccaccaga atcgaatata aaaggcgaac acctttccca attttggttt
      421 ctcctgaccc aaagacttta aatttaattt atttgtccct atttcaatca attgaacaac
      481 tatttcgaaa cgatgagatt tccttcaatt tttactgctg ttttattcgc agcatcctcc
      541 gcattagctg ctccagtcaa cactacaaca gaagatgaaa cggcacaaat tccggctgaa
      601 gctgtcatcg gttactcaga tttagaaggg gatttcgatg ttgctgtttt gccattttcc
      661 aacagcacaa ataacgggtt attgtttata aatactacta ttgccagcat tgctgctaaa
      721 gaagaagggg tatctctcga gaaaagagag gctgaagctg aattcacgtg gcccagccgg
      781 ccgtctcgga tcggtacctc gagccgcggc ggccgccagc tttctagaac aaaaactcat
      841 ctcagaagag gatctgaata gcgccgtcga ccatcatcat catcatcatt gagttttagc
      901 cttagacatg actgttcctc agttcaagtt gggcacttac gagaagaccg gtcttgctag
      961 attctaatca agaggatgtc agaatgccat ttgcctgaga gatgcaggct tcatttttga
     1021 tactttttta tttgtaacct atatagtata ggattttttt tgtcattttg tttcttctcg
     1081 tacgagcttg ctcctgatca gcctatctcg cagctgatga atatcttgtg gtaggggttt
     1141 gggaaaatca ttcgagtttg atgtttttct tggtatttcc cactcctctt cagagtacag
     1201 aagattaagt gagaccttcg tttgtgcgga tcccccacac accatagctt caaaatgttt
     1261 ctactccttt tttactcttc cagattttct cggactccgc gcatcgccgt accacttcaa
     1321 aacacccaag cacagcatac taaatttccc ctctttcttc ctctagggtg tcgttaatta
     1381 cccgtactaa aggtttggaa aagaaaaaag agaccgcctc gtttcttttt cttcgtcgaa
     1441 aaaggcaata aaaattttta tcacgtttct ttttcttgaa aatttttttt tttgattttt
     1501 ttctctttcg atgacctccc attgatattt aagttaataa acggtcttca atttctcaag
     1561 tttcagtttc atttttcttg ttctattaca acttttttta cttcttgctc attagaaaga
     1621 aagcatagca atctaatcta agggcggtgt tgacaattaa tcatcggcat agtatatcgg
     1681 catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc
     1741 ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt
     1801 ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt
     1861 catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg
     1921 cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc
     1981 ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg
     2041 cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtccgacg
     2101 cggcccgacg ggtccgaggc ctcggagatc cgtccccctt ttcctttgtc gatatcatgt
     2161 aattagttat gtcacgctta cattcacgcc ctccccccac atccgctcta accgaaaagg
     2221 aaggagttag acaacctgaa gtctaggtcc ctatttattt ttttatagtt atgttagtat
     2281 taagaacgtt atttatattt caaatttttc ttttttttct gtacagacgc gtgtacgcat
     2341 gtaacattat actgaaaacc ttgcttgaga aggttttggg acgctcgaag gctttaattt
     2401 gcaagctgga gaccaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     2461 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     2521 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     2581 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     2641 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     2701 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     2761 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     2821 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     2881 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     2941 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     3001 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     3061 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     3121 gttaagggat tttggtcatg catgagatc
//
