LOCUS       V006091                 5439 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006091
VERSION     V006091
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5439)
  AUTHORS   van Nierop GP, de Vries AA, Holkers M, Vrijsen KR, Goncalves MA.
  TITLE     Stimulation of homology-directed gene targeting at an endogenous
            human locus by a nicking endonuclease
  JOURNAL   Nucleic Acids Res. 37 (17), 5725-5736 (2009)
   PUBMED   19651880
REFERENCE   2  (bases 1 to 5439)
  AUTHORS   van Nierop GP, de Vries AA, Holkers M, Vrijsen KR, Goncalves MA.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUL-2009) Molecular Cell Biology, Leiden University
            Medical Center, Einthovenweg 20, Leiden, Zuid Holland 2333 ZC, The
            Netherlands
REFERENCE   3  (bases 1 to 5439)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5439)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res."; date: "2009"; volume: "37"; issue: "17"; pages:
            "5725-5736"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (14-JUL-2009) Molecular Cell Biology, Leiden University Medical
            Center, Einthovenweg 20, Leiden, Zuid Holland 2333 ZC, The
            Netherlands"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             8..1615
                     /gene="Rep68"
                     /label="Protein Rep68"
                     /note="Protein Rep68 from Adeno-associated virus 2 (isolate
                     Srivastava/1982). Accession#: P03132"
     polyA_signal    1688..1736
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(2223..2239)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2713..2817
                     /label="AmpR promoter"
     CDS             2818..3675
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3849..4437
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    4725..4746
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4761..4791
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    4799..4815
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4823..4839
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 ggccgccatg ccggggtttt acgagattgt gattaaggtc cccagcgacc ttgacgagca
       61 tctgcccggc atttctgaca gctttgtgaa ctgggtggcc gagaaggaat gggagttgcc
      121 gccagattct gacatggatc tgaatctgat tgagcaggca cccctgaccg tggccgagaa
      181 gctgcagcgc gactttctga cggaatggcg ccgtgtgagt aaggccccgg aggccctttt
      241 ctttgtgcaa tttgagaagg gagagagcta cttccacatg cacgtgctcg tggaaaccac
      301 cggggtgaaa tccatggttt tgggacgttt cctgagtcag attcgcgaaa aactgattca
      361 gagaatttac cgcgggatcg agccgacttt gccaaactgg ttcgcggtca caaagactcg
      421 caacggcgcc ggcggtggaa acaaggtggt ggatgagtgc tacatcccca attacttgct
      481 ccccaaaacc cagcctgagc tccaatgggc atggaccaac atggaacagt acctctgcgc
      541 ctgtttgaat ctcacggagc gtaaacggtt ggtggcgcag catctgacgc acgtgtcgca
      601 gacgcaggag cagaacaaag agaatcagaa tcccaattct gatgcgccgg tgatcagatc
      661 aaaaacttca gccaggtacg gggagctggt cgggtggctc gtggacaagg ggattacctc
      721 ggagaagcag tggatccagg aggaccaggc ctcatacatc tccttcaatg cggcctccaa
      781 ctcgcggtcc caaatcaagg ctgccttgga caatgcggga aagattatga gcctgactaa
      841 aaccgccccc gactacctgg tgggccagca gcccgtggag gacatttcca gcaatcggat
      901 ttataaaatt ttggaactaa acgggtacga tccccaatat gcggcttccg tctttctggg
      961 atgggccacg aaaaagttcg gcaagaggaa caccatctgg ctgtttgggc ctgcaactac
     1021 cgggaagacc aacatcgcgg aggccatagc ccacactgtg cccttctacg ggtgcgtaaa
     1081 ctggaccaat gagaactttc ccttcaacga ctgtgtcgac aagatggtga tctggtggga
     1141 ggaggggaag atgaccgcca aggtcgtgga gtcggccaaa gccattctcg gaggaagcaa
     1201 ggtgcgcgtg gaccagaaat gcaagtcctc ggcccagata gacccgactc ccgtgatcgt
     1261 cacctccaac accaacatgt gcgccgtgat tgacgggaac tcaacgacct tcgaacacca
     1321 gcagccgttg caagaccgga tgttcaaatt tgaactcacc cgccgtctgg atcatgactt
     1381 tgggaaggtc accaagcagg aagtcaaaga ctttttccgg tgggcaaagg atcacgtggt
     1441 tgaggtggag catgaattct acgtcaaaaa gggtggagcc aagaaaagac ccgcccccag
     1501 tgacgcagat ataagtgagc ccaaacgggt gcgcgagtca gttgcgcagc catcgacgtc
     1561 agacgcggaa gcttcgatca actacgcaga cagattggct cgaggacact ctctctgaag
     1621 atcgggagat gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
     1681 gctatgacgg caataaaaag acagaataaa acgcacgggt gttgggtcgt ttgttcataa
     1741 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattggga
     1801 ccaatacgcc cgcgtttctt ccttttcccc accccaaccc ccaagttcgg gtgaaggccc
     1861 agggctcgca gccaacgtcg gggcggcaag ccctgccata gccacgggcc ccgtgggtta
     1921 gggacggggt cccccatggg gaatggttta tggttcgtgg gggttattat tttgggcgtt
     1981 gcgtggggtc aggtccacga ctggactgag cagacagacc catggttttt ggatggcctg
     2041 ggcatggacc gcatgtactg gcgcgacacg aacaccgggc gtctgtggct gccaaacacc
     2101 cccgaccccc aaaaaccacc gcgcggattt ctggcgataa gcttgatatc gaattcctgc
     2161 agcccgatct gtactgaaat gtgtgggcgt ggcttaaggg atttaaatgt ttaaacgaat
     2221 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     2281 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     2341 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc
     2401 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
     2461 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     2521 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     2581 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     2641 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     2701 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     2761 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     2821 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     2881 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     2941 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     3001 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     3061 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     3121 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     3181 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     3241 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     3301 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     3361 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     3421 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     3481 gcccttccgg ctggctggtt tattgctgat aaatctggag cccgtgagcg tgggtctcgc
     3541 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     3601 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     3661 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     3721 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     3781 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3841 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3901 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3961 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     4021 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     4081 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     4141 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     4201 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     4261 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     4321 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     4381 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     4441 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     4501 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     4561 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     4621 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     4681 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     4741 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     4801 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcttgtt
     4861 taaacattta aatctcgatc gagccatgga tatcgcatgt ctggatctcg aagcttcgag
     4921 gagaagttcc ccaactttcc cgcctctcag cctttgaaag aaagaaaggg gagggggcag
     4981 gccgcgtgca gccgcgagcg gtgctgggct ccggctccaa ttccccatct cagtcgttcc
     5041 caaagtcctc ctgtttcatc caagcgtgta agggtccccg tccttgactc cctagtgtcc
     5101 tgctgcccac agtccagtcc tgggaaccag caccgatcac ctcccatcgg gccaatctca
     5161 gtcccttccc cctacgtcgg ggcccacacg ctcggtgcgt gcccagttga accaggcggc
     5221 tgcggaaaaa aaaaagcggg gagaaagtag ggcccggcta ctagcggttt tacgggcgca
     5281 cgtagctcag gcctcaagac cttgggctgg gactggctga gcctggcggg aggcggggtc
     5341 cgagtcaccg cctgccgccg cgcccccggt ttctataaat tgagcccgca gcctcccgct
     5401 tcgctctctg ctcctcctgc tcgacgggcc cggtaccgc
//
