LOCUS       V006095                 4716 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006095
VERSION     V006095
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Loset GA, Roos N, Bogen B, Sandlie I.
  TITLE     Expanding the versatility of phage display II: improved affinity
            selection of folded domains on protein VII and IX of the filamentous
            phage
  JOURNAL   PLoS ONE 6 (2), E17433 (2011)
   PUBMED   21390283
REFERENCE   2  (bases 1 to 4716)
  AUTHORS   Loset GA, Roos N, Bogen B, Sandlie I.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2010) Department of Molecular Biosciences,
            University of Oslo, PO Box 1041 Blindern, Oslo N-0316, Norway
REFERENCE   3  (bases 1 to 4716)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4716)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2011"; volume: "6"; issue: "2"; pages: "E17433"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (10-OCT-2010) Department of Molecular Biosciences, University of
            Oslo, PO Box 1041 Blindern, Oslo N-0316, Norway"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    31..52
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        67..97
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    105..121
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             140..151
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     misc_feature    164..907
                     /gene="scTCR-pIX"
                     /label="Region: scTCR"
                     /note="Region: scTCR"
     CDS             935..1030
                     /label="M13 gene IX"
                     /note="pIX"
     terminator      complement(1054..1097)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(1118..1141)
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             complement(1142..1951)
                     /gene="fkpA"
                     /label="FKBP-type peptidyl-prolyl cis-trans isomerase FkpA"
                     /note="FKBP-type peptidyl-prolyl cis-trans isomerase FkpA
                     from Escherichia coli (strain K12). Accession#: P45523"
     rep_origin      2249..2704
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2730..2834
                     /label="AmpR promoter"
     CDS             2835..3692
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3866..4454
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 ctcgagagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
       61 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
      121 atttcacaca gaattcatta aagaggagaa attaaccatg gcccagcaga aggtgcagca
      181 gagcccagaa tccctcattg tcccagaggg agccatgact tctctcaact gcactttcag
      241 cgacagtgct tctcagtatt ttgcatggta cagacagcaa tctgggaaag cccccaaggc
      301 actgatgtcc atcttctcca atggtgaaaa agaagaaggc agattcacaa ttcacctcaa
      361 taaagccagt ctgcatttct cgctacacat cagagactcc cagcccagtg actctgctct
      421 ctacctctgt gcagtgaggg gccctaatac aggaaactac aaatacgtct ttggagcagg
      481 taccagactg aaggttatag caaagctttc agggagtgca tccgccccaa aacttgaaga
      541 aggtgaattt tcagaagcac gcgtagaggc tgcagtcacc caaagcccaa gaaacaaggt
      601 ggcagtaaca ggaggaaagg tgacattgag ctgtaatcag actaataacc acaacaacat
      661 gtactggtat cggcaggaca cggggcatgg gctgaggctg atccattatt catatggtgc
      721 tggcagcact gagaaaggag atatccctga tggatacaag gcctccagac caagccaaga
      781 gaacttctcc ctcattctgg agttggctac cccctctcag acatcagtgt acttctgtgc
      841 cagcggtgat gcgggacagg ggcactccga ctacaccttc ggctcaggga ccaggctttt
      901 ggtaatagcg gccgctggat ccaaagatat cagaatgagt gttttagtgt attctttcgc
      961 ctctttcgtt ttaggttggt gccttcgtag tggcattacg tattttaccc gtttaatgga
     1021 aacttcctca tgataagcta gcttgaggca tcaataaaac gaaaggctca gtcgaaagac
     1081 tgggcctttc attttatctg ttgtttgtcg gttaacgctt gtcgtcatcg tccttgtagt
     1141 cttttttagc agaatctgcg gctttcgcat cagcttccgg ctttgcatca gccttcggcg
     1201 ctggtttcac atccagcagc tctacgtcaa acaccagggt agaattcggt gggatccccg
     1261 gaacacccgc tttgccgtaa gccagttctg gtggaataac cagtttgatc ttaccgcctt
     1321 tcttgatgtt cttcagacct tctgtccaac ccgggataac accgtccaga cggaaagaaa
     1381 gcggttcacc acgggtgtaa gagttgtcga actctttacc gtcgatcagc gtacctttgt
     1441 agttcactac aacagtatcg ctgtctttcg gtgcttcgcc tttaccggct tctactacct
     1501 gataaaccag accagttgaa gaggttttca cacctttctc tttggcaaat ttctcgcggt
     1561 actctttacc ttttgcttcg ttatcagccg cgtctttttc catcttcgcc tgagcagaag
     1621 acttcacgcg agcttcgaat gcttgtagag tctgttcgat ctcttggtcg gagagtttgc
     1681 tcttatcagc aaatgcatcc tgaacaccag cgatcagctg atctttatcc agtttgatgc
     1741 ccagtttttc ttgttctttt agagagtttt ccatgtaacg acccagcgag gcacccagtg
     1801 cataagctga tttctgatcg tcatttttga acgctgcttt gctgtcagca gctgtagcag
     1861 gttttgcagc ttcagcagca aaagtgattg gtgcatgcag ggcaacggcc attgtggtcg
     1921 ccagcagcgt tactttaaac agtgatttca tccatatctc caggatcggg gcatctcacc
     1981 ccagggttaa ctattatcag aagggtacta taaagcgttg tcgaacaaat ctacatacag
     2041 acacgcccta ttatcatcta ttttcagact ctttttgttt aaattagttt cgatgaccgc
     2101 gaaatgagtg ctgtctcggg cagcaaagtt aagtagaatc cgcggcgacc attcgacaaa
     2161 agaggtgaas agtcgacctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     2221 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     2281 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     2341 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     2401 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     2461 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     2521 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     2581 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     2641 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     2701 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     2761 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     2821 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     2881 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     2941 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     3001 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     3061 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     3121 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     3181 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     3241 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     3301 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     3361 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     3421 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     3481 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     3541 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     3601 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     3661 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     3721 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     3781 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     3841 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3901 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3961 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     4021 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     4081 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     4141 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     4201 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     4261 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     4321 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     4381 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     4441 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     4501 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     4561 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     4621 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     4681 attaatgcag gtatcacgag gccctttcgt cttcac
//
