LOCUS       40924_21065        3676 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Phagemid vector pGALD9DL, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Loset GA, Roos N, Bogen B, Sandlie I.
  TITLE     Expanding the versatility of phage display II: improved affinity 
            selection of folded domains on protein VII and IX of the filamentous
            phage
  JOURNAL   PLoS ONE 6 (2), E17433 (2011)
  PUBMED    21390283
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Loset GA, Roos N, Bogen B, Sandlie I.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2010) Department of Molecular Biosciences, 
            University of Oslo, PO Box 1041 Blindern, Oslo N-0316, Norway
REFERENCE   3  (bases 1 to 3676)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3676)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2011"; volume: "6"; issue: "2"; pages: "E17433"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-OCT-2010) Department of Molecular Biosciences, University of 
            Oslo, PO Box 1041 Blindern, Oslo N-0316, Norway"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3676
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    31..52
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        67..97
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    105..121
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             140..151
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    164..922
                     /gene="scFv-pIX"
                     /label=Region: scFv
                     /note="Region: scFv"
     CDS             950..1045
                     /label=M13 gene IX
                     /note="pIX"
     terminator      complement(1069..1112)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      1209..1664
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1690..1794
                     /label=AmpR promoter
     CDS             1795..2652
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2826..3414
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctcgagagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
       61 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
      121 atttcacaca gaattcatta aagaggagaa attaaccatg gcccaggtgc agctggtgca
      181 gtctggaggt gaggtgaaga agcctggggc ctcagtgaag gtctcctgca aggcttctgg
      241 ttacaccttt accaactatg gcatcacctg ggtgcgacag gcccctggac aagggcttga
      301 gtggatggga tggatcagcg cgtacaatgg ttacaaaagt tatgcacaga acttccaggg
      361 cagagtcacc atgaccacag acacattcac gagtacagcc tacatggagc tgaggaccct
      421 gagatctgac gacacggccg tgtattactg tgtgagactc gtccccaaaa gaaccgctac
      481 ccttcactac tacatagacg tctggggcaa agggaccacg gtcaccgtct cctcagggag
      541 tgcatccgcc ccaaagcttg aagaaggtga attttcagaa gcacgcgtac agtctgtgtt
      601 gactcagccg ccctcagtgt ctgcggcccc aggacagaag gtcaccatct cctgctctgg
      661 aagcagctcc aacattggaa ataattatgt ctcctggtac gttcaactcc caggaacagc
      721 ccccaaactc ctcatttatg acaataataa gcgattctca ggagttcctg accgattctc
      781 tggctccaag tctggcacgt cagccaccct gggcatcacc gggctccaga ctggggacga
      841 ggccgattat tactgcggag catgggatgg cagcctgcgt gaagcggtat tcggcggagg
      901 gaccaaggtc accgtcctag gtgcggccgc tggatccaaa gatatcagaa tgagtgtttt
      961 agtgtattct ttcgcctctt tcgttttagg ttggtgcctt cgtagtggca ttacgtattt
     1021 tacccgttta atggaaactt cctcatgata agctagcttg aggcatcaat aaaacgaaag
     1081 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggttaa cgtcgacctg
     1141 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     1201 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     1261 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     1321 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     1381 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     1441 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     1501 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     1561 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     1621 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     1681 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     1741 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     1801 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     1861 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     1921 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     1981 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     2041 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     2101 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     2161 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     2221 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     2281 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     2341 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     2401 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     2461 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     2521 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     2581 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     2641 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     2701 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     2761 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2821 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2881 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2941 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     3001 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3061 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3121 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3181 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3241 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3301 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3361 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3421 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3481 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     3541 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     3601 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag gtatcacgag
     3661 gccctttcgt cttcac
//
