LOCUS       40924_21060        3736 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Phagemid vector pGALD9, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Loset GA, Roos N, Bogen B, Sandlie I.
  TITLE     Expanding the versatility of phage display II: improved affinity 
            selection of folded domains on protein VII and IX of the filamentous
            phage
  JOURNAL   PLoS ONE 6 (2), E17433 (2011)
  PUBMED    21390283
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Loset GA, Roos N, Bogen B, Sandlie I.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2010) Department of Molecular Biosciences, 
            University of Oslo, PO Box 1041 Blindern, Oslo N-0316, Norway
REFERENCE   3  (bases 1 to 3736)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3736)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2011"; volume: "6"; issue: "2"; pages: "E17433"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-OCT-2010) Department of Molecular Biosciences, University of 
            Oslo, PO Box 1041 Blindern, Oslo N-0316, Norway"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3736
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    31..52
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        67..97
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    105..121
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             140..151
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     sig_peptide     158..223
                     /label=pelB signal sequence
                     /note="leader peptide for secretion"
     misc_feature    224..982
                     /gene="pelBss/scFv-pIX fusion"
                     /label=Region: scFv
                     /note="Region: scFv"
     CDS             1010..1105
                     /label=M13 gene IX
                     /note="pIX"
     terminator      complement(1129..1172)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      1269..1724
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1750..1854
                     /label=AmpR promoter
     CDS             1855..2712
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2886..3474
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctcgagagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
       61 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
      121 atttcacaca gaattcatta aagaggagaa attaaccatg aaatacctat tgcctacggc
      181 agccgctggc ttgctgctgc tggcagctca gccggccatg gcccaggtgc agctggtgca
      241 gtctggaggt gaggtgaaga agcctggggc ctcagtgaag gtctcctgca aggcttctgg
      301 ttacaccttt accaactatg gcatcacctg ggtgcgacag gcccctggac aagggcttga
      361 gtggatggga tggatcagcg cgtacaatgg ttacaaaagt tatgcacaga acttccaggg
      421 cagagtcacc atgaccacag acacattcac gagtacagcc tacatggagc tgaggaccct
      481 gagatctgac gacacggccg tgtattactg tgtgagactc gtccccaaaa gaaccgctac
      541 ccttcactac tacatagacg tctggggcaa agggaccacg gtcaccgtct cctcagggag
      601 tgcatccgcc ccaaagcttg aagaaggtga attttcagaa gcacgcgtac agtctgtgtt
      661 gactcagccg ccctcagtgt ctgcggcccc aggacagaag gtcaccatct cctgctctgg
      721 aagcagctcc aacattggaa ataattatgt ctcctggtac gttcaactcc caggaacagc
      781 ccccaaactc ctcatttatg acaataataa gcgattctca ggagttcctg accgattctc
      841 tggctccaag tctggcacgt cagccaccct gggcatcacc gggctccaga ctggggacga
      901 ggccgattat tactgcggag catgggatgg cagcctgcgt gaagcggtat tcggcggagg
      961 gaccaaggtc accgtcctag gtgcggccgc tggatccaaa gatatcagaa tgagtgtttt
     1021 agtgtattct ttcgcctctt tcgttttagg ttggtgcctt cgtagtggca ttacgtattt
     1081 tacccgttta atggaaactt cctcatgata agctagcttg aggcatcaat aaaacgaaag
     1141 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggttaa cgtcgacctg
     1201 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     1261 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     1321 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     1381 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     1441 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     1501 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     1561 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     1621 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     1681 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     1741 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     1801 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     1861 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     1921 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     1981 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     2041 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     2101 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     2161 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     2221 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     2281 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     2341 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     2401 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     2461 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     2521 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     2581 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     2641 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     2701 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     2761 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     2821 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2881 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2941 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3001 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     3061 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3121 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3181 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3241 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3301 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3361 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3421 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3481 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3541 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     3601 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     3661 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag gtatcacgag
     3721 gccctttcgt cttcac
//
