LOCUS       40924_21050        3679 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Phagemid vector pGALD7DL, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Loset GA, Roos N, Bogen B, Sandlie I.
  TITLE     Expanding the versatility of phage display II: improved affinity 
            selection of folded domains on protein VII and IX of the filamentous
            phage
  JOURNAL   PLoS ONE 6 (2), E17433 (2011)
  PUBMED    21390283
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Loset GA, Roos N, Bogen B, Sandlie I.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2010) Department of Molecular Biosciences, 
            University of Oslo, PO Box 1041 Blindern, Oslo N-0316, Norway
REFERENCE   3  (bases 1 to 3679)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3679)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2011"; volume: "6"; issue: "2"; pages: "E17433"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-OCT-2010) Department of Molecular Biosciences, University of 
            Oslo, PO Box 1041 Blindern, Oslo N-0316, Norway"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    31..52
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        67..97
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    105..121
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             140..151
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    164..922
                     /gene="scFv-pVII fusion"
                     /label=Region: scFv
                     /note="Region: scFv"
     CDS             950..1048
                     /label=M13 gene VII
                     /note="pVII"
     terminator      complement(1072..1115)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      1212..1667
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1693..1797
                     /label=AmpR promoter
     CDS             1798..2655
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2829..3417
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctcgagagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
       61 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
      121 atttcacaca gaattcatta aagaggagaa attaaccatg gcccaggtgc agctggtgca
      181 gtctggaggt gaggtgaaga agcctggggc ctcagtgaag gtctcctgca aggcttctgg
      241 ttacaccttt accaactatg gcatcacctg ggtgcgacag gcccctggac aagggcttga
      301 gtggatggga tggatcagcg cgtacaatgg ttacaaaagt tatgcacaga acttccaggg
      361 cagagtcacc atgaccacag acacattcac gagtacagcc tacatggagc tgaggaccct
      421 gagatctgac gacacggccg tgtattactg tgtgagactc gtccccaaaa gaaccgctac
      481 ccttcactac tacatagacg tctggggcaa agggaccacg gtcaccgtct cctcagggag
      541 tgcatccgcc ccaaagcttg aagaaggtga attttcagaa gcacgcgtac agtctgtgtt
      601 gactcagccg ccctcagtgt ctgcggcccc aggacagaag gtcaccatct cctgctctgg
      661 aagcagctcc aacattggaa ataattatgt ctcctggtac gttcaactcc caggaacagc
      721 ccccaaactc ctcatttatg acaataataa gcgattctca ggagttcctg accgattctc
      781 tggctccaag tctggcacgt cagccaccct gggcatcacc gggctccaga ctggggacga
      841 ggccgattat tactgcggag catgggatgg cagcctgcgt gaagcggtat tcggcggagg
      901 gaccaaggtc accgtcctag gtgcggccgc tggatccaaa gatatcagaa tggagcaggt
      961 cgcggatttc gacacaattt atcaggcgat gatacaaatc tccgttgtac tttgtttcgc
     1021 gcttggtata atcgctgggg gtcaaagatg ataagctagc ttgaggcatc aataaaacga
     1081 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt taacgtcgac
     1141 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     1201 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     1261 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     1321 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     1381 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     1441 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     1501 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     1561 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     1621 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     1681 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     1741 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     1801 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     1861 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     1921 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     1981 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     2041 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     2101 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     2161 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     2221 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     2281 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     2341 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     2401 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     2461 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     2521 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     2581 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     2641 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     2701 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     2761 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     2821 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     2881 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     2941 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     3001 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3061 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3121 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3181 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     3241 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     3301 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     3361 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     3421 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     3481 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     3541 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     3601 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg caggtatcac
     3661 gaggcccttt cgtcttcac
//
