LOCUS       40924_21045        3739 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Phagemid vector pGALD7, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Loset GA, Roos N, Bogen B, Sandlie I.
  TITLE     Expanding the versatility of phage display II: improved affinity 
            selection of folded domains on protein VII and IX of the filamentous
            phage
  JOURNAL   PLoS ONE 6 (2), E17433 (2011)
  PUBMED    21390283
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Loset GA, Roos N, Bogen B, Sandlie I.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2010) Department of Molecular Biosciences, 
            University of Oslo, PO Box 1041 Blindern, Oslo N-0316, Norway
REFERENCE   3  (bases 1 to 3739)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3739)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2011"; volume: "6"; issue: "2"; pages: "E17433"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-OCT-2010) Department of Molecular Biosciences, University of 
            Oslo, PO Box 1041 Blindern, Oslo N-0316, Norway"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3739
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    31..52
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        67..97
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    105..121
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             140..151
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     sig_peptide     158..223
                     /label=pelB signal sequence
                     /note="leader peptide for secretion"
     misc_feature    224..982
                     /gene="pelBss/scFv-pVII fusion"
                     /label=Region: scFv
                     /note="Region: scFv"
     CDS             1010..1108
                     /label=M13 gene VII
                     /note="pVII"
     terminator      complement(1132..1175)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      1272..1727
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1753..1857
                     /label=AmpR promoter
     CDS             1858..2715
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2889..3477
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctcgagagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
       61 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
      121 atttcacaca gaattcatta aagaggagaa attaaccatg aaatacctat tgcctacggc
      181 agccgctggc ttgctgctgc tggcagctca gccggccatg gcccaggtgc agctggtgca
      241 gtctggaggt gaggtgaaga agcctggggc ctcagtgaag gtctcctgca aggcttctgg
      301 ttacaccttt accaactatg gcatcacctg ggtgcgacag gcccctggac aagggcttga
      361 gtggatggga tggatcagcg cgtacaatgg ttacaaaagt tatgcacaga acttccaggg
      421 cagagtcacc atgaccacag acacattcac gagtacagcc tacatggagc tgaggaccct
      481 gagatctgac gacacggccg tgtattactg tgtgagactc gtccccaaaa gaaccgctac
      541 ccttcactac tacatagacg tctggggcaa agggaccacg gtcaccgtct cctcagggag
      601 tgcatccgcc ccaaagcttg aagaaggtga attttcagaa gcacgcgtac agtctgtgtt
      661 gactcagccg ccctcagtgt ctgcggcccc aggacagaag gtcaccatct cctgctctgg
      721 aagcagctcc aacattggaa ataattatgt ctcctggtac gttcaactcc caggaacagc
      781 ccccaaactc ctcatttatg acaataataa gcgattctca ggagttcctg accgattctc
      841 tggctccaag tctggcacgt cagccaccct gggcatcacc gggctccaga ctggggacga
      901 ggccgattat tactgcggag catgggatgg cagcctgcgt gaagcggtat tcggcggagg
      961 gaccaaggtc accgtcctag gtgcggccgc tggatccaaa gatatcagaa tggagcaggt
     1021 cgcggatttc gacacaattt atcaggcgat gatacaaatc tccgttgtac tttgtttcgc
     1081 gcttggtata atcgctgggg gtcaaagatg ataagctagc ttgaggcatc aataaaacga
     1141 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt taacgtcgac
     1201 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     1261 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     1321 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     1381 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     1441 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     1501 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     1561 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     1621 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     1681 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     1741 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     1801 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     1861 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     1921 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     1981 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     2041 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     2101 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     2161 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     2221 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     2281 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     2341 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     2401 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     2461 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     2521 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     2581 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     2641 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     2701 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     2761 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     2821 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     2881 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     2941 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3001 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     3061 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3121 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3181 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3241 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     3301 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     3361 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     3421 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     3481 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     3541 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     3601 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     3661 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg caggtatcac
     3721 gaggcccttt cgtcttcac
//
