LOCUS       V006114                 6640 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006114
VERSION     V006114
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6640)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6640)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6640)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6640)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6640
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      133..380
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     primer_bind     complement(403..419)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(427..443)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(451..481)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(496..517)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(817..1405)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1579..2436)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2437..2541)
                     /label="AmpR promoter"
     CDS             3402..4211
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
     regulatory      4593..5805
                     /label="CaGAL1 promoter"
                     /note="CaGAL1 promoter"
                     /regulatory_class="promoter"
     CDS             5873..6433
                     /gene="IFNB1"
                     /label="Interferon beta"
                     /note="Interferon beta from Homo sapiens. Accession#:
                     P01574"
     misc_feature    6437..6636
                     /label="3' UTR of hIFNbeta"
                     /note="3' UTR of hIFNbeta"
ORIGIN
        1 gaggcctagc ggccgcctag aggatccccg ggcgctaggc ggccgctagg cctttttggc
       61 caagctcgaa tttcgaggaa ttcgagctcg gtacccgggg gatcgatccg tccccctttt
      121 cctttgtcga tatcatgtaa ttagttatgt cacgcttaca ttcacgccct ccccccacat
      181 ccgctctaac cgaaaaggaa ggagttagac aacctgaagt ctaggtccct atttattttt
      241 ttatagttat gttagtatta agaacgttat ttatatttca aatttttctt ttttttctgt
      301 acagacgcgt gtacgcatgt aacattatac tgaaaacctt gcttgagaag gttttgggac
      361 gctcgaaggc tttaatttgc aagctagctt ggcgtaatca tggtcatagc tgtttcctgt
      421 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
      481 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
      541 tttccagtcg ggaaacctgt cgtgccagag atctctgcat taatgaatcg gccaacgcgc
      601 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
      661 ctcggtcgtt cggctgcggc gagcggtatc agatcgatct cactcaaagg cggtaatacg
      721 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
      781 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
      841 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
      901 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
      961 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     1021 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     1081 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     1141 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     1201 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     1261 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     1321 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     1381 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     1441 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     1501 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     1561 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     1621 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     1681 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     1741 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     1801 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     1861 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     1921 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     1981 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     2041 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     2101 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     2161 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     2221 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     2281 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     2341 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     2401 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     2461 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     2521 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     2581 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     2641 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     2701 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     2761 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     2821 tatcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
     2881 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
     2941 ccggccacgg ggcctgccac catacccacg ccgaaacaag cactaatagg aattgatttg
     3001 gatggtataa acggaaacaa aaaaaagagc tggtactact ttctttaaaa ttattttatt
     3061 atttgatttt atttaatagt atatattata ttttgaacgt agattatttt gttgaaagtt
     3121 gctgtagtgc cattgattcg taacactaat tctgtattag tcattcctct tgtttgatag
     3181 tatccaaaaa aacggctatt tttttgcaat cttatttcct gcatattata cagataacat
     3241 aatgaaagaa aaaatctttt tttttgttct tcaatgatga tttcaaccat tcttttaaac
     3301 attgatcaat tcctgagcaa caaccccata cacactggtt tatataccgc cccttttaca
     3361 gttgaagaaa gaaatagaaa tagaaatagc aaacaaaaga tatgacagtc aacactaaga
     3421 cctatagtga gagagcagaa actcatgcct caccagtagc acagcgatta tttcgattaa
     3481 tggaactgaa gaaaaccaat ttatgtgcat caattgacgt tgataccact aaggagttcc
     3541 tcgagttaat tgataaatta ggtccttatg tatgcttaat caagactcat attgatataa
     3601 tcaatgattt ttcctatgaa tccactattg aaccattatt agaactttca cgtaaacatc
     3661 aatttatgat ttttgaagat agaaaatttg ctgatattgg taataccgta aagaaacaat
     3721 atattggtgg agtttataaa attagtagtt gggcagatat taccaatgct catggtgtca
     3781 ctgggaatgg agtggttgaa ggattaaaac agggagctaa agaaaccacc accaaccaag
     3841 agccaagagg gttattgatg ttagctgaat tatcatcagt gggatcatta gcatatggag
     3901 aatattctca aaaaactgtt gaaattgcta aatccgataa ggaatttgtt attggattta
     3961 ttgcccaacg tgatatgggt ggccaagaag aaggatttga ttggcttatt atgacacctg
     4021 gagttggatt agatgataaa ggtgatggat taggacaaca atatagaact gttgatgaag
     4081 ttgttagcac tggaactgat attatcattg ttggtagagg attgtttggt aaaggaagag
     4141 atccagatat tgaaggtaaa aggtatagaa atgctggttg gaatgcttat ttgaaaaaga
     4201 ctggccaatt ataaatgtga agggggagat tttcacttta ttagatttgt atatatgtag
     4261 aataaataaa taaataagtt aaataaataa ttaaataagg gtggtaatta ttactattta
     4321 caatcaaagg tggtccttct agctgtaatc cgggcagcgc aacggaacat tcatcagtgt
     4381 aaaaatggaa tcaataaagc cctgcgctca tgagcccgaa gtggcgagcc cgatcttccc
     4441 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg cagcgcgcag
     4501 ggtcagcctg aatacgcgtt taatgaccag cacagtcgtg atggcaaggt cagaatagcc
     4561 caagtcggcc gaggggcctg tacagtgagg gaagatctga tattgacgaa gaggaaccaa
     4621 tgtaacgtta cactgaagaa aacacacaat aaacgggaag aaacggtgta aaagtgtgaa
     4681 aataattttt gaatatcatt tcccttggtt taattccaaa cgaaacgtgt tttttttaga
     4741 gaatgggaat tcttattgga tgtctagatt gtttgtttac tccagactgt gcacaaaaac
     4801 gtttggatgg atgatcagaa gatattttta ggcttagctc taaatataag aaatgatgct
     4861 tgaaaaacca gacagaaatt gagtttcaaa aattggtaat gtgaggtatt agtcaactaa
     4921 ccaaataaca atgcaaaccg gttgatacat ttcattttga aaataatgaa actggaattg
     4981 gatgaccagc acacaaacac ataaagtaat tatgggaatt agaagcgaac atagaggagt
     5041 acttggccac gaacagaata caagtgggaa cactattttc tccattgttt tagttctgtt
     5101 tttttgtcag cctagttttg tgctatgtgt aaaaaatatt gccaagaaaa aaagcttgtt
     5161 ttgtggccag tgtccgaaaa aaattttggg gaatcttcgg attaatttat gttttcattc
     5221 catcggggaa agtggggggg aaaaaatttt aagcagttca caaaaccttc caaaaaatat
     5281 atggacaaag atgattgtat tttcccgaca ccaaaatcat aattaattat gagaaagtta
     5341 aatgtaacgt tacaatttat gtttatttga aggtgaaaag cgatttatga tttttccgaa
     5401 atgaaaattt tttttaggtt tatttttttt gtcgggcaaa gaaaaactga acaaggatta
     5461 ttaaaatttt tggtgtttgt ttgtgtctgg agaattcatt cctctctcat cttcacacaa
     5521 tgtttagaca tctgacacga ttcatgatag ttcggtttcc ggggttggtg tttagttttc
     5581 gtttttcttt ttttttggaa agaatgtttt agctcattgg ttttctttct tcattcaata
     5641 gttttgaaag aatttgccca cttgttatta caatcatata aaattaaact ttgatataaa
     5701 atagagtttg aaagtttccc agatcctttt tgatttcttt gtaaattttt ttttctccca
     5761 catatacaca catacaaacc gatttttata agaaagagtt ataccctgca gctcgacctc
     5821 gactgtttaa acctgcaggc atgcaagctt ggccaaaaag gcctcgagga acatgaccaa
     5881 caagtgtctc ctccaaattg ctctcctgtt gtgcttctcc actacagctc tttccatgag
     5941 ctacaacttg cttggattcc tacaaagaag cagcaatttt cagtgtcaga agctcctgtg
     6001 gcaattgaat gggaggcttg aatactgcct caaggacagg atgaactttg acatccctga
     6061 ggagattaag cagctgcagc agttccagaa ggaggacgcc gcattgacca tctatgagat
     6121 gctccagaac atctttgcta ttttcagaca agattcatct agcactggct ggaatgagac
     6181 tattgttgag aacctcctgg ctaatgtcta tcatcagata aaccatctga agacagtcct
     6241 ggaagaaaaa ctggagaaag aagatttcac caggggaaaa ctcatgagca gtctgcacct
     6301 gaaaagatat tatgggagga ttctgcatta cctgaaggcc aaggagtaca gtcactgtgc
     6361 ctggaccata gtcagagtgg aaatcctaag gaacttttac ttcattaaca gacttacagg
     6421 ttacctccga aactgaagat ctcctagcct gtgcctctgg gactggacaa ttgcttcaag
     6481 cattcttcaa ccagcagatg ctgtttaagt gactgatggc taatgtactg catatgaaag
     6541 gacactagaa gattttgaaa tttttattaa attatgagtt atttttattt atttaaattt
     6601 tattttggaa aataaattat ttttggtgca aaagtccctc
//
