LOCUS       V006115                 7176 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006115
VERSION     V006115
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7176)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7176)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7176)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7176)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7176
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(10..28)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(46..62)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(70..86)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(94..124)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(139..160)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(448..1036)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1210..2067)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2068..2172)
                     /label="AmpR promoter"
     rep_origin      complement(2506..2961)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3102..3118
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        3125..3143
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3692..4501
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
     regulatory      4799..6011
                     /label="CaGAL1 promoter"
                     /note="CaGAL1 promoter"
                     /regulatory_class="promoter"
     misc_feature    complement(6069..6268)
                     /label="3' UTR of hIFNbeta"
                     /note="3' UTR of hIFNbeta"
     CDS             complement(6272..6832)
                     /gene="IFNB1"
                     /label="Interferon beta"
                     /note="Interferon beta from Homo sapiens. Accession#:
                     P01574"
     terminator      6926..7173
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
ORIGIN
        1 cttgagtatt ctatagtgtc acctaaatag cttggcgtaa tcatggtcat agctgtttcc
       61 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
      121 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
      181 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
      241 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
      301 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
      361 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
      421 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
      481 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
      541 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      601 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
      661 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
      721 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
      781 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
      841 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
      901 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
      961 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1021 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     1081 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     1141 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     1201 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     1261 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     1321 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     1381 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     1441 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     1501 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     1561 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     1621 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     1681 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     1741 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     1801 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     1861 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     1921 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     1981 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     2041 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     2101 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     2161 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     2221 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
     2281 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     2341 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     2401 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     2461 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgaaatt gtaaacgtta
     2521 atattttgtt aaaattcgcg ttaaatattt gttaaatcag ctcatttttt aaccaatagg
     2581 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     2641 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     2701 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc acccaaatca agttttttgc
     2761 ggtcgaggtg ccgtaaagct ctaaatcgga accctaaagg gagcccccga tttagagctt
     2821 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     2881 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     2941 atgcgccgct acagggcgcg tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     3001 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     3061 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     3121 attgtaatac gactcactat agggcgaatt ggttttccaa tgatgagcac ttttaaagtt
     3181 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
     3241 atacactatt ctcagaatga cttggttgag tactaatagg aattgatttg gatggtataa
     3301 acggaaacaa aaaaaagagc tggtactact ttctttaaaa ttattttatt atttgatttt
     3361 atttaatagt atatattata ttttgaacgt agattatttt gttgaaagtt gctgtagtgc
     3421 cattgattcg taacactaat tctgtattag tcattcctct tgtttgatag tatccaaaaa
     3481 aacggctatt tttttgcaat cttatttcct gcatattata cagataacat aatgaaagaa
     3541 aaaatctttt tttttgttct tcaatgatga tttcaaccat tcttttaaac attgatcaat
     3601 tcctgagcaa caaccccata cacactggtt tatatatcgc cccttttaca gttgaagaaa
     3661 gaaatagaaa tagaaatagc aaacaaaaga tatgacagtc aacactaaga cctatagtga
     3721 gagagcagaa actcatgcct caccagtagc acaacgatta tttcgattaa tggaactgaa
     3781 gaaaaccaat ttatgtgcat caattgatgt tgataccact aaggaattcc ttgaattaat
     3841 tgataaattg ggtccttatg tatgcttaat caagactcat attgatataa tcaatgattt
     3901 ttcctatgaa tccactattg aaccattatt agaactttca cgtaaacatc aatttatgat
     3961 ttttgaagat agaaaatttg ctgatattgg taataccgtg aagaaacaat atattggtgg
     4021 agtttataaa attagtagtt gggcagatat tactaatgct catggtgtca ctgggaatgg
     4081 agtagttgaa ggattaaaac agggagctaa agaaaccacc accaaccaag agccaagagg
     4141 gttattgatg ttagctgaat tatcatcagt gggatcatta gcatatggag aatattctca
     4201 aaaaactgtt gaaattgcta aatccgataa ggaatttgtt attggattta ttgcccaacg
     4261 tgatatgggt ggacaagaag aaggatttga ttggcttatt atgacacctg gagttggatt
     4321 agatgataaa ggtgatggat taggacaaca atatagaact gttgatgaag ttgttagcac
     4381 tggaactgat attatcattg ttggtagagg attgtttggt aaaggaagag atccagatat
     4441 tgaaggtaaa aggtatagag atgctggttg gaatgcttat ttgaaaaaaa ctggccaatt
     4501 ataaatgtga agggggagat tttcacttta ttagatttgt atatatgtat aataaataaa
     4561 taaataagtt aaataaataa ttaaataagg gtggtaatta ttactattta caatcaaagg
     4621 tggtccttct agctgtaatc cgggcagcgc aacggaacat tcatcagtgt aaaaatggaa
     4681 tcaataaagc cctgcgcagc gcgcagggtc agcctgaata cgcgtttaat gaccagcaca
     4741 gtcgtgatgg caaggtcaga atagcccaag tcggccgagg ggcctgtaca gtgagggaag
     4801 atctgatatt gacgaagagg aaccaatgta acgttacact gaagaaaaca cacaataaac
     4861 gggaagaaac ggtgtaaaag tgtgaaaata atttttgaat atcatttccc ttggtttaat
     4921 tccaaacgaa acgtgttttt tttagagaat gggaattctt attggatgtc tagattgttt
     4981 gtttactcca gactgtgcac aaaaacgttt ggatggatga tcagaagata tttttaggct
     5041 tagctctaaa tataagaaat gatgcttgaa aaaccagaca gaaattgagt ttcaaaaatt
     5101 ggtaatgtga ggtattagtc aactaaccaa ataacaatgc aaaccggttg atacatttca
     5161 ttttgaaaat aatgaaactg gaattggatg accagcacac aaacacataa agtaattatg
     5221 ggaattagaa gcgaacatag aggagtactt ggccacgaac agaatacaag tgggaacact
     5281 attttctcca ttgttttagt tctgtttttt tgtcagccta gttttgtgct atgtgtaaaa
     5341 aatattgcca agaaaaaaag cttgttttgt ggccagtgtc cgaaaaaaat tttggggaat
     5401 cttcggatta atttatgttt tcattccatc ggggaaagtg ggggggaaaa aattttaagc
     5461 agttcacaaa accttccaaa aaatatatgg acaaagatga ttgtattttc ccgacaccaa
     5521 aatcataatt aattatgaga aagttaaatg taacgttaca atttatgttt atttgaaggt
     5581 gaaaagcgat ttatgatttt tccgaaatga aaattttttt taggtttatt ttttttgtcg
     5641 ggcaaagaaa aactgaacaa ggattattaa aatttttggt gtttgtttgt gtctggagaa
     5701 ttcattcctc tctcatcttc acacaatgtt tagacatctg acacgattca tgatagttcg
     5761 gtttccgggg ttggtgttta gttttcgttt ttcttttttt ttggaaagaa tgttttagct
     5821 cattggtttt ctttcttcat tcaatagttt tgaaagaatt tgcccacttg ttattacaat
     5881 catataaaat taaactttga tataaaatag agtttgaaag tttcccagat cctttttgat
     5941 ttctttgtaa attttttttt ctcccacata tacacacata caaaccgatt tttataagaa
     6001 agagttatac cctgcagctc gacctcgagg gatccgggcc ctctagatgc ggccgctagg
     6061 cctcgaggga cttttgcacc aaaaataatt tattttccaa aataaaattt aaataaataa
     6121 aaataactca taatttaata aaaatttcaa aatcttctag tgtcctttca tatgcagtac
     6181 attagccatc agtcacttaa acagcatctg ctggttgaag aatgcttgaa gcaattgtcc
     6241 agtcccagag gcacaggcta ggagatcttc agtttcggag gtaacctgta agtctgttaa
     6301 tgaagtaaaa gttccttagg atttccactc tgactatggt ccaggcacag tgactgtact
     6361 ccttggcctt caggtaatgc agaatcctcc cataatatct tttcaggtgc agactgctca
     6421 tgagttttcc cctggtgaaa tcttctttct ccagtttttc ttccaggact gtcttcagat
     6481 ggtttatctg atgatagaca ttagccagga ggttctcaac aatagtctca ttccagccag
     6541 tgctagatga atcttgtctg aaaatagcaa agatgttctg gagcatctca tagatggtca
     6601 atgcggcgtc ctccttctgg aactgctgca gctgcttaat ctcctcaggg atgtcaaagt
     6661 tcatcctgtc cttgaggcag tattcaagcc tcccattcaa ttgccacagg agcttctgac
     6721 actgaaaatt gctgcttctt tgtaggaatc caagcaagtt gtagctcatg gaaagagctg
     6781 tagtggagaa gcacaacagg agagcaattt ggaggagaca cttgttggtc atgttcctcg
     6841 aggccttttt ggccaagctg gcgcctgctg cgcgacggcg agctgctcac cacccaggat
     6901 ccgtccccct tttcctttgt cgatatcatg taattagtta tgtcacgctt acattcacgc
     6961 cctcccccca catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc
     7021 cctatttatt tttttatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt
     7081 cttttttttc tgtacagacg cgtgtacgca tgtaacatta tactgaaaac cttgcttgag
     7141 aaggttttgg gacgctcgaa ggctttaatt tgcaag
//
