LOCUS       40924_20965        8018 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Integrative expression vector pGAL10lac, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8018)
  AUTHORS   Vickers CE, Bydder SF, Nielsen LK.
  TITLE     Yeast expression vectors with dominant markers and dual gene 
            expression cassettes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8018)
  AUTHORS   Bydder SF, Vickers CE.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2013) AIBN, The University of Queensland, Crn 
            College and Cooper Roads (Bldg 75), St Lucia, QLD 4072, Australia
REFERENCE   3  (bases 1 to 8018)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8018)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-JUL-2013) AIBN, The University of Queensland, Crn College and 
            Cooper Roads (Bldg 75), St Lucia, QLD 4072, Australia"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8018
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2243..2261
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2290..2306
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(2345..2997)
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     CDS             3019..6090
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG
                     QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQ
                     ISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
                     ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
                     GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLW
                     YTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
                     LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
                     KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSL
                     IKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQ
                     TIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ
                     LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELG
                     NKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWK
                     AAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDV
                     EVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY
                     VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDG
                     FHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     terminator      6094..6341
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             complement(6808..7608)
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(7609..7829)
                     /label=URA3 promoter
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttt cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggca gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg ggggaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggccgag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctc gaaattaacc ctcactaaag ggaacaaaag ctggtaccgg
     2281 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccggggg
     2341 atccgttttt tctccttgac gttaaagtat agaggtatat taacaatttt ttgttgatac
     2401 ttttatgaca tttgaataag aagtaataca aactgaaaat gttgaaagta ttagttaaag
     2461 tggttatgca gcttttccat ttatatatct gttaatagat caaaaatcat cgcttcgctg
     2521 attaattacc ccagaaataa ggctaaaaaa ctaatcgcat tatcatccta tggttgttaa
     2581 tttgattcgt taatttgaag gtttgtgggg ccaggttact gccaattttt cctcttcata
     2641 accataaaag ctagtattgt agaatcttta ttgttcggag cagtgcggcg cgaggcacat
     2701 ctgcgtttca ggaacgcgac cggtgaagac gaggacgcac ggaggagagt cttccgtcgg
     2761 agggctgtcg cccgctcggc ggcttctaat ccgtacttca atatagcaat gagcagttaa
     2821 gcgtattact gaaagttcca aagagaaggt ttttttaggc taagataatg gggctcttta
     2881 catttccaca acatataagt aagattagat atggatatgt atatggtggt aatgccatgt
     2941 aatatgatta ttaaacttct ttgcgtccat ccaaaaaaaa agtaagaatt tttgaaaatt
     3001 ctctagagcg gccgcaccat gaccatgatt acggattcac tggccgtcgt tttacaacgt
     3061 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     3121 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     3181 ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg
     3241 ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac
     3301 ggttacgatg cgcccatcta caccaacgtg acctatccca ttacggtcaa tccgccgttt
     3361 gttcccacgg agaatccgac gggttgttac tcgctcacat ttaatgttga tgaaagctgg
     3421 ctacaggaag gccagacgcg aattattttt gatggcgtta actcggcgtt tcatctgtgg
     3481 tgcaacgggc gctgggtcgg ttacggccag gacagtcgtt tgccgtctga atttgacctg
     3541 agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga tggtgctgcg ctggagtgac
     3601 ggcagttatc tggaagatca ggatatgtgg cggatgagcg gcattttccg tgacgtctcg
     3661 ttgctgcata aaccgactac acaaatcagc gatttccatg ttgccactcg ctttaatgat
     3721 gatttcagcc gcgctgtact ggaggctgaa gttcagatgt gcggcgagtt gcgtgactac
     3781 ctacgggtaa cagtttcttt atggcagggt gaaacgcagg tcgccagcgg caccgcgcct
     3841 ttcggcggtg aaattatcga tgagcgtggt ggttatgccg atcgcgtcac actacgtctg
     3901 aacgtcgaaa acccgaaact gtggagcgcc gaaatcccga atctctatcg tgcggtggtt
     3961 gaactgcaca ccgccgacgg cacgctgatt gaagcagaag cctgcgatgt cggtttccgc
     4021 gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca agccgttgct gattcgaggc
     4081 gttaaccgtc acgagcatca tcctctgcat ggtcaggtca tggatgagca gacgatggtg
     4141 caggatatcc tgctgatgaa gcagaacaac tttaacgccg tgcgctgttc gcattatccg
     4201 aaccatccgc tgtggtacac gctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc
     4261 aatattgaaa cccacggcat ggtgccaatg aatcgtctga ccgatgatcc gcgctggcta
     4321 ccggcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg
     4381 atcatctggt cgctggggaa tgaatcaggc cacggcgcta atcacgacgc gctgtatcgc
     4441 tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc
     4501 acggccaccg atattatttg cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg
     4561 gctgtgccga aatggtccat caaaaaatgg ctttcgctac ctggagagac gcgcccgctg
     4621 atcctttgcg aatacgccca cgcgatgggt aacagtcttg gcggtttcgc taaatactgg
     4681 caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg tctgggactg ggtggatcag
     4741 tcgctgatta aatatgatga aaacggcaac ccgtggtcgg cttacggcgg tgattttggc
     4801 gatacgccga acgatcgcca gttctgtatg aacggtctgg tctttgccga ccgcacgccg
     4861 catccagcgc tgacggaagc aaaacaccag cagcagtttt tccagttccg tttatccggg
     4921 caaaccatcg aagtgaccag cgaatacctg ttccgtcata gcgataacga gctcctgcac
     4981 tggatggtgg cgctggatgg taagccgctg gcaagcggtg aagtgcctct ggatgtcgct
     5041 ccacaaggta aacagttgat tgaactgcct gaactaccgc agccggagag cgccgggcaa
     5101 ctctggctca cagtacgcgt agtgcaaccg aacgcgaccg catggtcaga agccgggcac
     5161 atcagcgcct ggcagcagtg gcgtctggcg gaaaacctca gtgtgacgct ccccgccgcg
     5221 tcccacgcca tcccgcatct gaccaccagc gaaatggatt tttgcatcga gctgggtaat
     5281 aagcgttggc aatttaaccg ccagtcaggc tttctttcac agatgtggat tggcgataaa
     5341 aaacaactgc tgacgccgct gcgcgatcag ttcacccgtg caccgctgga taacgacatt
     5401 ggcgtaagtg aagcgacccg cattgaccct aacgcctggg tcgaacgctg gaaggcggcg
     5461 ggccattacc aggccgaagc agcgttgttg cagtgcacgg cagatacact tgctgatgcg
     5521 gtgctgatta cgaccgctca cgcgtggcag catcagggga aaaccttatt tatcagccgg
     5581 aaaacctacc ggattgatgg tagtggtcaa atggcgatta ccgttgatgt tgaagtggcg
     5641 agcgatacac cgcatccggc gcggattggc ctgaactgcc agctggcgca ggtagcagag
     5701 cgggtaaact ggctcggatt agggccgcaa gaaaactatc ccgaccgcct tactgccgcc
     5761 tgttttgacc gctgggatct gccattgtca gacatgtata ccccgtacgt cttcccgagc
     5821 gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc
     5881 gacttccagt tcaacatcag ccgctacagt caacagcaac tgatggaaac cagccatcgc
     5941 catctgctgc acgcggaaga aggcacatgg ctgaatatcg acggtttcca tatggggatt
     6001 ggtggcgacg actcctggag cccgtcagta tcggcggaat tccagctgag cgccggtcgc
     6061 taccattacc agttggtctg gtgtcaaaaa taatcatgta attagttatg tcacgcttac
     6121 attcacgccc tccccccaca tccgctctaa ccgaaaagga aggagttaga caacctgaag
     6181 tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta tttatatttc
     6241 aaatttttct tttttttctg tacagacgcg tgtacgcatg taacattata ctgaaaacct
     6301 tgcttgagaa ggttttggga cgctcgaagg ctttaatttg cggcccccgg ctttccccgt
     6361 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     6421 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     6481 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     6541 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     6601 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     6661 ttaacgttta caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     6721 accgcagggt aataactgat ataattaaat tgaagctcta atttgtgagt ttagtataca
     6781 tgcatttact tataatacag ttttttagtt ttgctggccg catcttctca aatatgcttc
     6841 ccagcctgct tttctgtaac gttcaccctc taccttagca tcccttccct ttgcaaatag
     6901 tcctcttcca acaataataa tgtcagatcc tgtagagacc acatcatcca cggttctata
     6961 ctgttgaccc aatgcgtctc ccttgtcatc taaacccaca ccgggtgtca taatcaacca
     7021 atcgtaacct tcatctcttc cacccatgtc tctttgagca ataaagccga taacaaaatc
     7081 tttgtcgctc ttcgcaatgt caacagtacc cttagtatat tctccagtag atagggagcc
     7141 cttgcatgac aattctgcta acatcaaaag gcctctaggt tcctttgtta cttcttctgc
     7201 cgcctgcttc aaaccgctaa caatacctgg gcccaccaca ccgtgtgcat tcgtaatgtc
     7261 tgcccattct gctattctgt atacacccgc agagtactgc aatttgactg tattaccaat
     7321 gtcagcaaat tttctgtctt cgaagagtaa aaaattgtac ttggcggata atgcctttag
     7381 cggcttaact gtgccctcca tggaaaaatc agtcaagata tccacatgtg tttttagtaa
     7441 acaaattttg ggacctaatg cttcaactaa ctccagtaat tccttggtgg tacgaacatc
     7501 caatgaagca cacaagtttg tttgcttttc gtgcatgata ttaaatagct tggcagcaac
     7561 aggactagga tgagtagcag cacgttcctt atatgtagct ttcgacatga tttatcttcg
     7621 tttcctgcag gtttttgttc tgtgcagttg ggttaagaat actgggcaat ttcatgtttc
     7681 ttcaacacta catatgcgta tatataccaa tctaagtctg tgctccttcc ttcgttcttc
     7741 cttctgttcg gagattaccg aatcaaaaaa atttcaaaga aaccgaaatc aaaaaaaaga
     7801 ataaaaaaaa aatgatgaat tgaattgaaa agcgtggtgc actctcagta caatctgctc
     7861 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     7921 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     7981 gtgtcagagg ttttcaccgt catcaccgaa acgcgcga
//
