LOCUS       V012967                 6824 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V012967
VERSION     V012967
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6824)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6824
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        242..260
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(271..3174)
                     /gene="PKD2"
                     /label="Polycystin-2"
                     /note="Polycystin-2 from Homo sapiens. Accession#: Q13563"
     promoter        complement(3197..3215)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3222..3238)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             3376..3675
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             4027..4818
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(5074..5931)
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6055..6643
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca
      241 gaattaaccc tcactaaagg gactagttca tacgtggaca ttagaactcc catttccacc
      301 tgcaccttcc atgccttctg tagattggga ggaagatggg cgggagcttc tggggcgagg
      361 ttgaccattt agtcccactg gcgtgcctaa accatgactg atctgggaag ctgcatcatc
      421 ggattcccag cgttccaact cttcacgtac tagccgttcc atctgttccc tatggatttc
      481 actgtcacga cccagccttt catcctcggc caccccatcc aacagccttc ccagcacctc
      541 cctcctcttc agtttggctc gctccataat ctctagcttc acgatcacgg cgtcaatctt
      601 ggacactatg ctgccgatgg aatgctccat ccggtccact cgtctcacca ggacttgaaa
      661 ctcttcgtaa gaaacgccac tagaaatgct tcccctcctt ctggagctat gtccgctatc
      721 ttcatcgtca tcctcctcag agtcatccag gcttcgaggg aaacttcggc tgctcatggg
      781 acgtggtaaa gaactgtgat ccaaatccag gtcctccctc tctttctcca agtcgtctct
      841 catctgctga tgttcatgtt cggtcagttc ttggtctcca tcttggtcgt actttgtgaa
      901 tattgcctca atctctgcat cagtatggcc cttccctttg agatcttgtc gaagttcgtc
      961 aaagtttaac ttgcctcctc cttgccgcag actctctgaa atgtcatcca cggtattttt
     1021 tttcagtttt agtttgacca aagctttatg gtagcccttt ctgataagat ctgagagttc
     1081 catttcagct ttctgctgtg ccaagtcaga tttcacttca gagtaagtat cattgatgat
     1141 agccaaaaac atattcaaaa gaatgaagaa cataaagaac acaaatgtag tgaaataaat
     1201 tggtcccaaa actcgattag cttcctcaat ctctgcaaag ttgatatcgc ccaaaatgat
     1261 acggaattga gtgaagatac actcttggaa agtactgaag tcatcgacct gagtgccaaa
     1321 gacaaggtat gccaactgag catacgctag gaaaataatg aagaacataa tagcaaagcc
     1381 aaacaggtct ttggcacatc gagacatggt tgtcgagagc tggctcatgg tcctgttaaa
     1441 attgatgaat ttgaagagct taatccagac aaaaaatact gtgacagcag ctatattgtt
     1501 gaactgtatc tgccaatatg ccagatgctc aaagttgggg aaagtatttt gatcttccag
     1561 aaactgtagt agcacctcca catttgatgt tctgtatatg ttaattccta tagctaccac
     1621 tgacagcaca acgatcacaa catccagaca attccagaaa ctcctgaaat agtgtagttt
     1681 gtgaatgcga atttccaata tctcttccac cacatagtaa aagataaaga aacaaaagat
     1741 aatctcacag gctgccagga agaaatcaaa agttgtgaca tatcggatca gctttaaagg
     1801 ctgaaattgc caagatggaa tcacaccacc tgttgctggg aattcaacca ataacctgac
     1861 cacacagaac aggttaatgt tggcgttgta cactgagaag tcaataaaag ttgccctggt
     1921 tcctcggtcc agccagacat ttttcttgag gctagcaact tgtgcagctg tttcctctct
     1981 tgttcttgac aaatccagat aatagccagc tccactataa gttgcaatga ttccccagtg
     2041 gctactacca ttcaagtctt tttcacttgt gtagatccaa gcggttccat ttcggggccc
     2101 aaagggagcc ctatcttcac tactgacaga gtagacatca tagcactctt taatttcatc
     2161 tctcaagtcc tgggggatag agcaggatcc atttctgact cggagttgcc gtattcgtgg
     2221 aacccctaac agcaggttct catagaagat gaaacttcgg ttgtcagctt cagtctggtt
     2281 gctgggctgc atcttccagt acagcccatc caataaggag ccttctgtga acttccagaa
     2341 gtcttccatg gaagacagag ttttaaagtt agttttctcc gttttggaca cgggggtgtc
     2401 taggaagagc tgtgacatca tccgggtgta gtagtacaca ttggagctca tcatgccgta
     2461 ggtcaagatg cacaagacta tgagaaaaag gaggtatgtg accagttccc gtaaaacact
     2521 tttaaggtat ttctctcggt tagtgctgct ttcctccatg agtcttgttc cccagagacc
     2581 tcgcagcccg cgaaccagcc tctccgccca ggccactcgg ggcggctgcc cttccagggg
     2641 gaggtggcga tgcagcgggt ccccgccgcc gactgggctg gggcacggcg ggccctggtc
     2701 ctctcgccgg cgccgcctcc cgctcgggtg gcccgcgccg tggtagcccc caagcccccg
     2761 gctccgcgcg cccacggagc tcacggccga ggaggcggcc gacctccggc tgcccgggcg
     2821 ccactctacg tccatctcca ccaccattcc gccttcttcc ccttccacct cctcctcctc
     2881 ctcctcggcc tcgaagccgg ggttatcgcg gctccacgcc tgccgggagc acgacgagag
     2941 cggaggagaa ggggaggccg cggctccggc cggggggtcc cgcgcggccg cctgccggat
     3001 gcgctgcatc tcgatctcca ggccccgctg ctcgcagagg ccgcccgggg cggcgaggct
     3061 ggcgcccacg gccgcgcagc cagccatcag ccggcccggg tccggcgcgc ggggcgcggg
     3121 cggccgcttg gcgtccccgg gctgctgagg ctgcacgcga ctggagttca ccatgcggcc
     3181 gctaaattca attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa
     3241 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     3301 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     3361 agcctatacg tacggcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg
     3421 tttgtggatg tacagagtga tattattgac acgccggggc gacggatggt gatccccctg
     3481 gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc
     3541 ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc
     3601 ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg
     3661 atgttctggg gaatataaat gtcaggcatg agattatcaa aaaggatctt cacctagatc
     3721 cttttcacgt agaaagccag tccgcagaaa cggtgctgac cccggatgaa tgtcagctac
     3781 tgggctatct ggacaaggga aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg
     3841 cttacatggc gatagctaga ctgggcggtt ttatggacag caagcgaacc ggaattgcca
     3901 gctggggcgc cctctggtaa ggttgggaag ccctgcaaag taaactggat ggctttcttg
     3961 ccgccaagga tctgatggcg caggggatca agctctgatc aagagacagg atgaggatcg
     4021 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     4081 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     4141 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     4201 gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     4261 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     4321 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     4381 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     4441 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     4501 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg
     4561 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     4621 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     4681 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     4741 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     4801 cttcttgacg agttcttctg aattattaac gcttacaatt tcctgatgcg gtattttctc
     4861 cttacgcatc tgtgcggtat ttcacaccgc atcaggtggc acttttcggg gaaatgtgcg
     4921 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagatt
     4981 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     5041 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     5101 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     5161 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     5221 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     5281 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     5341 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     5401 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     5461 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     5521 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     5581 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     5641 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     5701 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     5761 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     5821 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     5881 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     5941 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgaccaaaa tcccttaacg
     6001 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     6061 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     6121 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     6181 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     6241 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     6301 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     6361 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     6421 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     6481 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     6541 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     6601 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     6661 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     6721 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     6781 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaag
//
