LOCUS       V006118                 7868 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006118
VERSION     V006118
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7868)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7868)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7868)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7868)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..406
                     /label="ADH1 promoter"
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             452..472
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             488..829
                     /label="GAL4 activation domain"
                     /note="activation domain of the GAL4 transcriptional
                     activator"
     CDS             875..1573
                     /gene="Bcl2l1"
                     /label="Bcl-2-like protein 1"
                     /note="Bcl-2-like protein 1 from Mus musculus. Accession#:
                     Q64373"
     misc_feature    1577..2033
                     /label="3UTR"
                     /note="3UTR"
     terminator      2450..2637
                     /label="ADH1 terminator"
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(2758..3849)
                     /label="LEU2"
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     promoter        complement(3850..4254)
                     /label="LEU2 promoter"
     primer_bind     complement(4296..4312)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4320..4336)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4344..4374)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4389..4410)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4698..5286)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5460..6317)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6318..6422)
                     /label="AmpR promoter"
     rep_origin      6704..7868
                     /label="2u ori"
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 gcttgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc
       61 atatccgcaa tgacaaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga
      121 cagcaccaac agatgtcgtt gttccagagc tgatgagggg tatcttcgaa cacacgaaac
      181 tttttccttc cttcattcac gcacactact ctctaatgag caacggtata cggccttcct
      241 tccagttact tgaatttgaa ataaaaaaag tttgccgctt tgctatcaag tataaataga
      301 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt
      361 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gctttgcaaa
      421 gatggataaa gcggaattaa ttcccgagcc tccaaaaaag aagagaaagg tcgaattggg
      481 taccgccgcc aattttaatc aaagtgggaa tattgctgat agctcattgt ccttcacttt
      541 cactaacagt agcaacggtc cgaacctcat aacaactcaa acaaattctc aagcgctttc
      601 acaaccaatt gcctcctcta acgttcatga taacttcatg aataatgaaa tcacggctag
      661 taaaattgat gatggtaata attcaaaacc actgtcacct ggttggacgg accaaactgc
      721 gtataacgcg tttggaatca ctacagggat gtttaatacc actacaatgg atgatgtata
      781 taactatcta ttcgatgatg aagatacccc accaaaccca aaaaaagaga tcgaattcgg
      841 cacgagactg gttgagccca tctctattat aaaaatgtct cagagcaacc gggagctggt
      901 ggtcgacttt ctctcctaca agctttccca gaaaggatac agctggagtc agtttagtga
      961 tgtcgaagag aataggactg aggccccaga agaaactgaa gcagagaggg agacccccag
     1021 tgccatcaat ggcaacccat cctggcacct ggcggatagc ccggccgtga atggagccac
     1081 tggccacagc agcagtttgg atgcgcggga ggtgattccc atggcagcag tgaagcaagc
     1141 gctgagagag gcaggcgatg agtttgaact gcggtaccgg agagcgttca gtgatctaac
     1201 atcccagctt cacataaccc cagggaccgc gtatcagagc tttgagcagg tagtgaatga
     1261 actctttcgg gatggagtaa actggggtcg catcgtggcc tttttctcct ttggcggggc
     1321 actgtgcgtg gaaagcgtag acaaggagat gcaggtattg gtgagtcgga ttgcaagttg
     1381 gatggccacc tatctgaatg accacctaga gccttggatc caggagaacg gcggctggga
     1441 cacttttgtg gatctctacg ggaacaatgc agcagccgag agccggaaag gcaaggaggg
     1501 cttcaaccgc tggtttctga cgggcatgac tgtggctggt gtggttctgc tgggctcact
     1561 cttcagtcgg aagtgaccag acactgaccg tccactcacc tctcacctcc caccctgccc
     1621 ccaccacaac tctctcttca gccaccattg ctaccaggag aaccactaca tgcaactcac
     1681 gccccttgcc ctatcacagg gttgggccta gacggagtcc cctgcaatta gctttctagg
     1741 atctaccaca cttctgtgaa agccaccttc cccccacatc tcagttctct tggcctcaaa
     1801 actcacaagg ttttccccta aaccagctcc ttggaggctg gcaggaacag agaggggtgt
     1861 gtagggagga gagcctgtct tgttggtggg accctaatta cccctgagcc ttctcgggaa
     1921 tgcttttcca gcagggagct cttcccctga aggagactag cttgccttgg ttttgatgtg
     1981 tgtggcctca gacgtgatca tttcccatcc gctgtgtccc tgggtctccc tccctcgtgc
     2041 cgaattcccg gggatccgtc gacctgcaga gatctatgaa tcgtagatac tgaaaaaccc
     2101 cgcaagttca cttcaactgt gcatcgtgca ccatctcaat ttctttcatt tatacatcgt
     2161 tttgccttct tttatgtaac tatactcctc taagtttcaa tcttggccat gtaacctctg
     2221 atctatagaa ttttttaaat gactagaatt aatgcccatc ttttttttgg acctaaattc
     2281 ttcatgaaaa tatattacga gggcttattc agaagctttg gacttcttcg ccagaggttt
     2341 ggtcaagtct ccaatcaagg ttgtcggctt gtctaccttg ccagaaattt acgaaaagat
     2401 ggaaaagggt caaatcgttg gtagatacgt tgttgacact tctaaataag cgaatttctt
     2461 atgatttatg atttttatta ttaaataagt tataaaaaaa ataagtgtat acaaatttta
     2521 aagtgactct taggttttaa aacgaaaatt cttgttcttg agtaactctt tcctgtaggt
     2581 caggttgctt tctcaggtat agcatgaggt cgctcttatt gaccacacct ctaccggcat
     2641 gcccgaaatt cccctaccct atgaacatat tccattttgt aatttcgtgt cgtttctatt
     2701 atgaatttca tttataaagt ttatgtacaa atatcataaa aaaagagaat ctttttaagc
     2761 aaggattttc ttaacttctt cggcgacagc atcaccgact tcggtggtac tgttggaacc
     2821 acctaaatca ccagttctga tacctgcatc caaaaccttt ttaactgcat cttcaatggc
     2881 cttaccttct tcaggcaagt tcaatgacaa tttcaacatc attgcagcag acaagatagt
     2941 ggcgataggg tcaaccttat tctttggcaa atctggagca gaaccgtggc atggttcgta
     3001 caaaccaaat gcggtgttct tgtctggcaa agaggccaag gacgcagatg gcaacaaacc
     3061 caaggaacct gggataacgg aggcttcatc ggagatgata tcaccaaaca tgttgctggt
     3121 gattataata ccatttaggt gggttgggtt cttaactagg atcatggcgg cagaatcaat
     3181 caattgatgt tgaaccttca atgtaggaaa ttcgttcttg atggtttcct ccacagtttt
     3241 tctccataat cttgaagagg ccaaaacatt agctttatcc aaggaccaaa taggcaatgg
     3301 tggctcatgt tgtagggcca tgaaagcggc cattcttgtg attctttgca cttctggaac
     3361 ggtgtattgt tcactatccc aagcgacacc atcaccatcg tcttcctttc tcttaccaaa
     3421 gtaaatacct cccactaatt ctctgacaac aacgaagtca gtacctttag caaattgtgg
     3481 cttgattgga gataagtcta aaagagagtc ggatgcaaag ttacatggtc ttaagttggc
     3541 gtacaattga agttctttac ggatttttag taaaccttgt tcaggtctaa cactacctgt
     3601 accccattta ggaccaccca cagcacctaa caaaacggca tcaaccttct tggaggcttc
     3661 cagcgcctca tctggaagtg ggacacctgt agcatcgata gcagcaccac caattaaatg
     3721 attttcgaaa tcgaacttga cattggaacg aacatcagaa atagctttaa gaaccttaat
     3781 ggcttcggct gtgatttctt gaccaacgtg gtcacctggc aaaacgacga tcttcttagg
     3841 ggcagacatt agaatggtat atccttgaaa tatatatata tattgctgaa atgtaaaagg
     3901 taagaaaagt tagaaagtaa gacgattgct aaccacctat tggaaaaaac aataggtcct
     3961 taaataatat tgtcaacttc aagtattgtg atgcaagcat ttagtcatga acgcttctct
     4021 attctatatg aaaagccggt tccggcctct cacctttcct ttttctccca atttttcagt
     4081 tgaaaaaggt atatgcgtca ggcgacctct gaaattaaca aaaaatttcc agtcatcgaa
     4141 tttgattctg tgcgatagcg cccctgtgtg ttctcgttat gttgaggaaa aaaataatgg
     4201 ttgctaagag attcgaactc ttgcatctta cgatacctga gtattcccac agttggggat
     4261 ctcgactcta gctagaggat caattcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     4321 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     4381 ggtgcctaat gagtgaggta actcacatta attgcgttgc gctcactgcc cgctttccag
     4441 tcgggaaacc tgtcgtgcca gctggattaa tgaatcggcc aacgcgcggg gagaggcggt
     4501 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     4561 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     4621 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     4681 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     4741 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     4801 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     4861 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     4921 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     4981 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     5041 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     5101 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     5161 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     5221 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     5281 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     5341 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     5401 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     5461 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     5521 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     5581 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     5641 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     5701 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     5761 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     5821 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     5881 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     5941 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     6001 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     6061 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     6121 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     6181 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     6241 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     6301 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     6361 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     6421 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     6481 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     6541 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     6601 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     6661 aactatgcgg catcagagca gattgtactg agagtgcacc ataacgcatt taagcataaa
     6721 cacgcactat gccgttcttc tcatgtatat atatatacag gcaacacgca gatataggtg
     6781 cgacgtgaac agtgagctgt atgtgcgcag ctcgcgttgc attttcggaa gcgctcgttt
     6841 tcggaaacgc tttgaagttc ctattccgaa gttcctattc tctagctaga aagtatagga
     6901 acttcagagc gcttttgaaa accaaaagcg ctctgaagac gcactttcaa aaaaccaaaa
     6961 acgcaccgga ctgtaacgag ctactaaaat attgcgaata ccgcttccac aaacattgct
     7021 caaaagtatc tctttgctat atatctctgt gctatatccc tatataacct acccatccac
     7081 ctttcgctcc ttgaacttgc atctaaactc gacctctaca ttttttatgt ttatctctag
     7141 tattactctt tagacaaaaa aattgtagta agaactattc atagagtgaa tcgaaaacaa
     7201 tacgaaaatg taaacatttc ctatacgtag tatatagaga caaaatagaa gaaaccgttc
     7261 ataattttct gaccaatgaa gaatcatcaa cgctatcact ttctgttcac aaagtatgcg
     7321 caatccacat cggtatagaa tataatcggg gatgccttta tcttgaaaaa atgcacccgc
     7381 agcttcgcta gtaatcagta aacgcgggaa gtggagtcag gcttttttta tggaagagaa
     7441 aatagacacc aaagtagcct tcttctaacc ttaacggacc tacagtgcaa aaagttatca
     7501 agagactgca ttatagagcg cacaaaggag aaaaaaagta atctaagatg ctttgttaga
     7561 aaaatagcgc tctcgggatg catttttgta gaacaaaaaa gaagtataga ttctttgttg
     7621 gtaaaatagc gctctcgcgt tgcatttctg ttctgtaaaa atgcagctca gattctttgt
     7681 ttgaaaaatt agcgctctcg cgttgcattt ttgttttaca aaaatgaagc acagattctt
     7741 cgttggtaaa atagcgcttt cgcgttgcat ttctgttctg taaaaatgca gctcagattc
     7801 tttgtttgaa aaattagcgc tctcgcgttg catttttgtt ctacaaaatg aagcacagat
     7861 gcttcgtt
//
