LOCUS       40924_20945        9637 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast two-hybrid vector pGADCg, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9637)
  AUTHORS   Stellberger T, Uetz P.
  TITLE     Yeast two-hybrid vectors with C-terminal fusion of Gal4 activation 
            and DNA-binding domains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9637)
  AUTHORS   Stellberger T, Uetz P.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2009) Institut fuer Toxikologie und Genetik, 
            Forschungszentrum Karlsruhe, Hermann-von-Helmholtz-Platz 1, 
            Eggenstein-Leopoldshafen, Baden-Wuerttemberg 76344, Germany
REFERENCE   3  (bases 1 to 9637)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9637)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-FEB-2009) Institut fuer Toxikologie und Genetik, 
            Forschungszentrum Karlsruhe, Hermann-von-Helmholtz-Platz 1, 
            Eggenstein-Leopoldshafen, Baden-Wuerttemberg 76344, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9637
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        771..1476
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             1522..1542
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     protein_bind    1561..1680
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1710..1740
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1794..2450
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2795..3097
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(3141..3265)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3279..3620
                     /codon_start=1
                     /label=GAL4 activation domain
                     /note="activation domain of the GAL4 transcriptional
                     activator"
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     terminator      4065..4252
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(4372..5463)
                     /codon_start=1
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(5464..5869)
                     /label=LEU2 promoter
     primer_bind     complement(5911..5927)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5935..5951)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5959..5989)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6004..6025)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     protein_bind    6080..6113
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(6191..6224)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     rep_origin      complement(6464..7052)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7226..8083)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8084..8188)
                     /label=AmpR promoter
     rep_origin      8470..9634
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 tgcatgcctg caggtcgaga tccgggatcg aagaaatgat ggtaaatgaa ataggaaatc
       61 aaggagcatg aaggcaaaag acaaatataa gggtcgaacg aaaaataaag tgaaaagtgt
      121 tgatatgatg tatttggctt tgcggcgccg aaaaaacgag tttacgcaat tgcacaatca
      181 tgctgactct gtggcggacc cgcgctcttg ccggcccggc gataacgctg ggcgtgaggc
      241 tgtgcccggc ggagtttttt gcgcctgcat tttccaaggt ttaccctgcg ctaaggggcg
      301 agattggaga agcaataaga atgccggttg gggttgcgat gatgacgacc acgacaactg
      361 gtgtcattat ttaagttgcc gaaagaacct gagtgcattt gcaacatgag tatactagaa
      421 gaatgagcca agacttgcga gacgcgagtt tgccggtggt gcgaacaata gagcgaccat
      481 gaccttgaag gtgagacgcg cataaccgct agagtacttt gaagaggaaa cagcaatagg
      541 gttgctacca gtataaatag acaggtacat acaacactgg aaatggttgt ctgtttgagt
      601 acgctttcaa ttcatttggg tgtgcacttt attatgttac aatatggaag ggaactttac
      661 acttctccta tgcacatata ttaattaaag tccaatgcta gtagagaagg ggggtaacac
      721 ccctccgcgc tcttttccga tttttttcta aaccgtggaa tatttcggat atccttttgt
      781 tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca tacatcggga
      841 ttcctataat accttcgttg gtctccctaa catgtaggtg gcggagggga gatatacaat
      901 agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt acactgcctc
      961 attgatggtg gtacataacg aactaatact gtagccctag acttgatagc catcatcata
     1021 tcgaagtttc actacccttt ttccatttgc catctattga agtaataata ggcgcatgca
     1081 acttcttttc tttttttttc ttttctctct cccccgttgt tgtctcacca tatccgcaat
     1141 gacaaaaaaa atgatggaag acactaaagg aaaaaattaa cgacaaagac agcaccaaca
     1201 gatgtcgttg ttccagagct gatgaggggt atctcgaagc acacgaaact ttttccttcc
     1261 ttcattcacg cacactactc tctaatgagc aacggtatac ggccttcctt ccagttactt
     1321 gaatttgaaa taaaaaaaag tttgctgtct tgctatcaag tataaataga cctgcaatta
     1381 ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt ctttttctgc
     1441 acaatatttc aagctatacc aagcatacaa tcaactccaa gctttgcaaa gatggataaa
     1501 gcggaattaa ttcccgagcc tccaaaaaag aagagaaagg tcgaattggg taccgcatca
     1561 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
     1621 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
     1681 tattggcggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg
     1741 tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga
     1801 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
     1861 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
     1921 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
     1981 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
     2041 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
     2101 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
     2161 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
     2221 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
     2281 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
     2341 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
     2401 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg
     2461 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
     2521 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca
     2581 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
     2641 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
     2701 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
     2761 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
     2821 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     2881 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
     2941 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     3001 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     3061 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
     3121 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
     3181 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     3241 gttcagcttt cttgtacaaa gtggttgatg gtaccgccgc caattttaat caaagtggga
     3301 atattgctga tagctcattg tccttcactt tcactaacag tagcaacggt ccgaacctca
     3361 taacaactca aacaaattct caagcgcttt cacaaccaat tgcctcctct aacgttcatg
     3421 ataacttcat gaataatgaa atcacggcta gtaaaattga tgatggtaat aattcaaaac
     3481 cactgtcacc tggttggacg gaccaaactg cgtataacgc gtttggaatc actacaggga
     3541 tgtttaatac cactacaatg gatgatgtat ataactatct attcgatgat gaagataccc
     3601 caccaaaccc aaaaaaagag atcctcgagt acgacgtacc agattacgct tagtgatcac
     3661 gatacgggat ccatcgagct cgagctgcag atgaatcgta gatactgaaa aaccccgcaa
     3721 gttcacttca actgtgcatc gtgcaccatc tcaatttctt tcatttatac atcgttttgc
     3781 cttcttttat gtaactatac tcctctaagt ttcaatcttg gccatgtaac ctctgatcta
     3841 tagaattttt taaatgacta gaattaatgc ccatcttttt tttggaccta aattcttcat
     3901 gaaaatatat tacgagggct tattcagaag ctttggactt cttcgccaga ggtttggtca
     3961 agtctccaat caaggttgtc ggcttgtcta ccttgccaga aatttacgaa aagatggaaa
     4021 agggtcaaat cgttggtaga tacgttgttg acacttctaa ataagcgaat ttcttatgat
     4081 ttatgatttt tattattaaa taagttataa aaaaaataag tgtatacaaa ttttaaagtg
     4141 actcttaggt tttaaaacga aaattcttat tcttgagtaa ctctttcctg taggtcaggt
     4201 tgctttctca ggtatagcat gaggtcgctc ttattgacca cacctctacc ggccggtcga
     4261 aattccccta ccctatgaac atattccatt ttgtaatttc gtgtcgtttc tattatgaat
     4321 ttcatttata aagtttatgt acaaatatca taaaaaaaga gaatcttttt aagcaaggat
     4381 tttcttaact tcttcggcga cagcatcacc gacttcggtg gtactgttgg aaccacctaa
     4441 atcaccagtt ctgatacctg catccaaaac ctttttaact gcatcttcaa tggccttacc
     4501 ttcttcaggc aagttcaatg acaatttcaa catcattgca gcagacaaga tagtggcgat
     4561 agggttgacc ttattctttg gcaaatctgg agcagaaccg tggcatggtt cgtacaaacc
     4621 aaatgcggtg ttcttgtctg gcaaagaggc caaggacgca gatggcaaca aacccaagga
     4681 acctgggata acggaggctt catcggagat gatatcacca aacatgttgc tggtgattat
     4741 aataccattt aggtgggttg ggttcttaac taggatcatg gcggcagaat caatcaattg
     4801 atgttgaacc ttcaatgtag gaaattcgtt cttgatggtt tcctccacag tttttctcca
     4861 taatcttgaa gaggccaaaa cattagcttt atccaaggac caaataggca atggtggctc
     4921 atgttgtagg gccatgaaag cggccattct tgtgattctt tgcacttctg gaacggtgta
     4981 ttgttcacta tcccaagcga caccatcacc atcgtcttcc tttctcttac caaagtaaat
     5041 acctcccact aattctctga caacaacgaa gtcagtacct ttagcaaatt gtggcttgat
     5101 tggagataag tctaaaagag agtcggatgc aaagttacat ggtcttaagt tggcgtacaa
     5161 ttgaagttct ttacggattt ttagtaaacc ttgttcaggt ctaacactac ctgtacccca
     5221 tttaggacca cccacagcac ctaacaaaac ggcatcaacc ttcttggagg cttccagcgc
     5281 ctcatctgga agtgggacac ctgtagcgtc gatagcagca ccaccaatta aatgattttc
     5341 gaaatcgaac ttgacattgg aacgaacatc agaaatagct ttaagaacct taatggcttc
     5401 ggctgtgatt tcttgaccaa cgtggtcacc tggcaaaacg acgatcttct taggggcaga
     5461 cattagaatg gtatatcctt gaaatatata tatatattgc tgaaatgtaa aaggtaagaa
     5521 aagttagaaa gtaagacgat tgctaaccac ctattggaaa aaacaatagg tccttaaata
     5581 atattgtcaa cttcaagtat tgtgatgcaa gcatttagtc atgaacgctt ctctattcta
     5641 tatgaaaagc cggttccggc gctctcacct ttcctttttc tcccaatttt tcagttgaaa
     5701 aaggtatatg cgtcaggcga cctctgaaat taacaaaaaa tttccagtca tcgaatttga
     5761 ttctgtgcga tagcgcccct gtgtgttctc gttatgttga ggaaaaaaat aatggttgct
     5821 aagagattcg aactcttgca tcttacgata cctgagtatt cccacagttg gggatctcga
     5881 ctctagctag aggatcaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     5941 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     6001 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     6061 aaacctgtcg tgccagctga taacttcgta taatgtatgc tatacgaagt tattaggtct
     6121 gaagaggagt ttacgtccag ccaagctagc ttggctgcag gtcgagcggc cgcgatccgg
     6181 aacccttaat ataacttcgt ataatgtatg ctatacgaag ttatcagctg cattaatgaa
     6241 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     6301 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     6361 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     6421 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     6481 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     6541 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     6601 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     6661 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     6721 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     6781 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     6841 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     6901 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     6961 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7021 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7081 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7141 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7201 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     7261 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     7321 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     7381 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     7441 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     7501 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     7561 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     7621 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     7681 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     7741 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     7801 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     7861 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     7921 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     7981 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     8041 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     8101 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8161 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     8221 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     8281 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     8341 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     8401 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     8461 tgcaccataa cgcatttaag cataaacacg cactatgccg ttcttctcat gtatatatat
     8521 atacaggcaa cacgcagata taggtgcgac gtgaacagtg agctgtatgt gcgcagctcg
     8581 cgttgcattt tcggaagcgc tcgttttcgg aaacgctttg aagttcctat tccgaagttc
     8641 ctattctcta gctagaaagt ataggaactt cagagcgctt ttgaaaacca aaagcgctct
     8701 gaagacgcac tttcaaaaaa ccaaaaacgc accggactgt aacgagctac taaaatattg
     8761 cgaataccgc ttccacaaac attgctcaaa agtatctctt tgctatatat ctctgtgcta
     8821 tatccctata taacctaccc atccaccttt cgctccttga acttgcatct aaactcgacc
     8881 tctacatttt ttatgtttat ctctagtatt actctttaga caaaaaaatt gtagtaagaa
     8941 ctattcatag agtgaatcga aaacaatacg aaaatgtaaa catttcctat acgtagtata
     9001 tagagacaaa atagaagaaa ccgttcataa ttttctgacc aatgaagaat catcaacgct
     9061 atcactttct gttcacaaag tatgcgcaat ccacatcggt atagaatata atcggggatg
     9121 cctttatctt gaaaaaatgc acccgcagct tcgctagtaa tcagtaaacg cgggaagtgg
     9181 agtcaggctt tttttatgga agagaaaata gacaccaaag tagccttctt ctaaccttaa
     9241 cggacctaca gtgcaaaaag ttatcaagag actgcattat agagcgcaca aaggagaaaa
     9301 aaagtaatct aagatgcttt gttagaaaaa tagcgctctc gggatgcatt tttgtagaac
     9361 aaaaaagaag tatagattct ttgttggtaa aatagcgctc tcgcgttgca tttctgttct
     9421 gtaaaaatgc agctcagatt ctttgtttga aaaattagcg ctctcgcgtt gcatttttgt
     9481 tttacaaaaa tgaagcacag attcttcgtt ggtaaaatag cgctttcgcg ttgcatttct
     9541 gttctgtaaa aatgcagctc agattctttg tttgaaaaat tagcgctctc gcgttgcatt
     9601 tttgttctac aaaatgaagc acagatgctt cgttgct
//
